Engineering and Applications of fungal laccases for organic synthesis

Laccases are multi-copper containing oxidases (EC 1.10.3.2), widely distributed in fungi, higher plants and bacteria. Laccase catalyses the oxidation of phenols, polyphenols and anilines by one-electron abstraction, with the concomitant reduction of oxygen to water in a four-electron transfer proces...

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Autores principales: Kunamneni, Adinarayana, Camarero, Susana, García-Burgos, Carlos, Plou, Francisco J, Ballesteros, Antonio, Alcalde, Miguel
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2613868/
https://www.ncbi.nlm.nih.gov/pubmed/19019256
http://dx.doi.org/10.1186/1475-2859-7-32
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author Kunamneni, Adinarayana
Camarero, Susana
García-Burgos, Carlos
Plou, Francisco J
Ballesteros, Antonio
Alcalde, Miguel
author_facet Kunamneni, Adinarayana
Camarero, Susana
García-Burgos, Carlos
Plou, Francisco J
Ballesteros, Antonio
Alcalde, Miguel
author_sort Kunamneni, Adinarayana
collection PubMed
description Laccases are multi-copper containing oxidases (EC 1.10.3.2), widely distributed in fungi, higher plants and bacteria. Laccase catalyses the oxidation of phenols, polyphenols and anilines by one-electron abstraction, with the concomitant reduction of oxygen to water in a four-electron transfer process. In the presence of small redox mediators, laccase offers a broader repertory of oxidations including non-phenolic substrates. Hence, fungal laccases are considered as ideal green catalysts of great biotechnological impact due to their few requirements (they only require air, and they produce water as the only by-product) and their broad substrate specificity, including direct bioelectrocatalysis. Thus, laccases and/or laccase-mediator systems find potential applications in bioremediation, paper pulp bleaching, finishing of textiles, bio-fuel cells and more. Significantly, laccases can be used in organic synthesis, as they can perform exquisite transformations ranging from the oxidation of functional groups to the heteromolecular coupling for production of new antibiotics derivatives, or the catalysis of key steps in the synthesis of complex natural products. In this review, the application of fungal laccases and their engineering by rational design and directed evolution for organic synthesis purposes are discussed.
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spelling pubmed-26138682009-01-06 Engineering and Applications of fungal laccases for organic synthesis Kunamneni, Adinarayana Camarero, Susana García-Burgos, Carlos Plou, Francisco J Ballesteros, Antonio Alcalde, Miguel Microb Cell Fact Review Laccases are multi-copper containing oxidases (EC 1.10.3.2), widely distributed in fungi, higher plants and bacteria. Laccase catalyses the oxidation of phenols, polyphenols and anilines by one-electron abstraction, with the concomitant reduction of oxygen to water in a four-electron transfer process. In the presence of small redox mediators, laccase offers a broader repertory of oxidations including non-phenolic substrates. Hence, fungal laccases are considered as ideal green catalysts of great biotechnological impact due to their few requirements (they only require air, and they produce water as the only by-product) and their broad substrate specificity, including direct bioelectrocatalysis. Thus, laccases and/or laccase-mediator systems find potential applications in bioremediation, paper pulp bleaching, finishing of textiles, bio-fuel cells and more. Significantly, laccases can be used in organic synthesis, as they can perform exquisite transformations ranging from the oxidation of functional groups to the heteromolecular coupling for production of new antibiotics derivatives, or the catalysis of key steps in the synthesis of complex natural products. In this review, the application of fungal laccases and their engineering by rational design and directed evolution for organic synthesis purposes are discussed. BioMed Central 2008-11-20 /pmc/articles/PMC2613868/ /pubmed/19019256 http://dx.doi.org/10.1186/1475-2859-7-32 Text en Copyright © 2008 Kunamneni et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review
Kunamneni, Adinarayana
Camarero, Susana
García-Burgos, Carlos
Plou, Francisco J
Ballesteros, Antonio
Alcalde, Miguel
Engineering and Applications of fungal laccases for organic synthesis
title Engineering and Applications of fungal laccases for organic synthesis
title_full Engineering and Applications of fungal laccases for organic synthesis
title_fullStr Engineering and Applications of fungal laccases for organic synthesis
title_full_unstemmed Engineering and Applications of fungal laccases for organic synthesis
title_short Engineering and Applications of fungal laccases for organic synthesis
title_sort engineering and applications of fungal laccases for organic synthesis
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2613868/
https://www.ncbi.nlm.nih.gov/pubmed/19019256
http://dx.doi.org/10.1186/1475-2859-7-32
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