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DNA-free RNA isolation protocols for Arabidopsis thaliana, including seeds and siliques

BACKGROUND: High throughput applications of the reverse transcriptase quantitative PCR (RT-qPCR) for quantification of gene expression demand straightforward procedures to isolate and analyze a considerable number of DNA-free RNA samples. Published protocols are labour intensive, use toxic organic c...

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Detalles Bibliográficos
Autores principales: Oñate-Sánchez, Luis, Vicente-Carbajosa, Jesús
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2613888/
https://www.ncbi.nlm.nih.gov/pubmed/18937828
http://dx.doi.org/10.1186/1756-0500-1-93