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Fat-to-glucose interconversion by hydrodynamic transfer of two glyoxylate cycle enzyme genes

The glyoxylate cycle, which is well characterized in higher plants and some microorganisms but not in vertebrates, is able to bypass the citric acid cycle to achieve fat-to-carbohydrate interconversion. In this context, the hydrodynamic transfer of two glyoxylate cycle enzymes, such as isocytrate ly...

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Autores principales: Cordero, P, Campion, J, Milagro, FI, Marzo, F, Martinez, JA
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2614421/
https://www.ncbi.nlm.nih.gov/pubmed/19077206
http://dx.doi.org/10.1186/1476-511X-7-49
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author Cordero, P
Campion, J
Milagro, FI
Marzo, F
Martinez, JA
author_facet Cordero, P
Campion, J
Milagro, FI
Marzo, F
Martinez, JA
author_sort Cordero, P
collection PubMed
description The glyoxylate cycle, which is well characterized in higher plants and some microorganisms but not in vertebrates, is able to bypass the citric acid cycle to achieve fat-to-carbohydrate interconversion. In this context, the hydrodynamic transfer of two glyoxylate cycle enzymes, such as isocytrate lyase (ICL) and malate synthase (MS), could accomplish the shift of using fat for the synthesis of glucose. Therefore, 20 mice weighing 23.37 ± 0.96 g were hydrodinamically gene transferred by administering into the tail vein a bolus with ICL and MS. After 36 hours, body weight, plasma glucose, respiratory quotient and energy expenditure were measured. The respiratory quotient was increased by gene transfer, which suggests that a higher carbohydrate/lipid ratio is oxidized in such animals. This application could help, if adequate protocols are designed, to induce fat utilization for glucose synthesis, which might be eventually useful to reduce body fat depots in situations of obesity and diabetes.
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spelling pubmed-26144212009-01-07 Fat-to-glucose interconversion by hydrodynamic transfer of two glyoxylate cycle enzyme genes Cordero, P Campion, J Milagro, FI Marzo, F Martinez, JA Lipids Health Dis Short Paper The glyoxylate cycle, which is well characterized in higher plants and some microorganisms but not in vertebrates, is able to bypass the citric acid cycle to achieve fat-to-carbohydrate interconversion. In this context, the hydrodynamic transfer of two glyoxylate cycle enzymes, such as isocytrate lyase (ICL) and malate synthase (MS), could accomplish the shift of using fat for the synthesis of glucose. Therefore, 20 mice weighing 23.37 ± 0.96 g were hydrodinamically gene transferred by administering into the tail vein a bolus with ICL and MS. After 36 hours, body weight, plasma glucose, respiratory quotient and energy expenditure were measured. The respiratory quotient was increased by gene transfer, which suggests that a higher carbohydrate/lipid ratio is oxidized in such animals. This application could help, if adequate protocols are designed, to induce fat utilization for glucose synthesis, which might be eventually useful to reduce body fat depots in situations of obesity and diabetes. BioMed Central 2008-12-10 /pmc/articles/PMC2614421/ /pubmed/19077206 http://dx.doi.org/10.1186/1476-511X-7-49 Text en Copyright © 2008 Cordero et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Paper
Cordero, P
Campion, J
Milagro, FI
Marzo, F
Martinez, JA
Fat-to-glucose interconversion by hydrodynamic transfer of two glyoxylate cycle enzyme genes
title Fat-to-glucose interconversion by hydrodynamic transfer of two glyoxylate cycle enzyme genes
title_full Fat-to-glucose interconversion by hydrodynamic transfer of two glyoxylate cycle enzyme genes
title_fullStr Fat-to-glucose interconversion by hydrodynamic transfer of two glyoxylate cycle enzyme genes
title_full_unstemmed Fat-to-glucose interconversion by hydrodynamic transfer of two glyoxylate cycle enzyme genes
title_short Fat-to-glucose interconversion by hydrodynamic transfer of two glyoxylate cycle enzyme genes
title_sort fat-to-glucose interconversion by hydrodynamic transfer of two glyoxylate cycle enzyme genes
topic Short Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2614421/
https://www.ncbi.nlm.nih.gov/pubmed/19077206
http://dx.doi.org/10.1186/1476-511X-7-49
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