Cargando…
Diagnostic Accuracy of NS1 ELISA and Lateral Flow Rapid Tests for Dengue Sensitivity, Specificity and Relationship to Viraemia and Antibody Responses
BACKGROUND: Dengue is a public health problem in many countries. Rapid diagnosis of dengue can assist patient triage and management. Detection of the dengue viral protein, NS1, represents a new approach to dengue diagnosis. METHODOLOGY/PRINCIPAL FINDINGS: The sensitivity and specificity of the Plate...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2009
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2614471/ https://www.ncbi.nlm.nih.gov/pubmed/19156192 http://dx.doi.org/10.1371/journal.pntd.0000360 |
_version_ | 1782163234655043584 |
---|---|
author | Ty Hang, Vu Minh Nguyet, Nguyen The Trung, Dinh Tricou, Vianney Yoksan, Sutee Minh Dung, Nguyen Van Ngoc, Tran Hien, Tran Tinh Farrar, Jeremy Wills, Bridget Simmons, Cameron P. |
author_facet | Ty Hang, Vu Minh Nguyet, Nguyen The Trung, Dinh Tricou, Vianney Yoksan, Sutee Minh Dung, Nguyen Van Ngoc, Tran Hien, Tran Tinh Farrar, Jeremy Wills, Bridget Simmons, Cameron P. |
author_sort | Ty Hang, Vu |
collection | PubMed |
description | BACKGROUND: Dengue is a public health problem in many countries. Rapid diagnosis of dengue can assist patient triage and management. Detection of the dengue viral protein, NS1, represents a new approach to dengue diagnosis. METHODOLOGY/PRINCIPAL FINDINGS: The sensitivity and specificity of the Platelia NS1 ELISA assay and an NS1 lateral flow rapid test (LFRT) were compared against a gold standard reference diagnostic algorithm in 138 Vietnamese children and adults. Overall, the Platelia NS1 ELISA was modestly more sensitive (82%) than the NS1 LFRT (72%) in confirmed dengue cases. Both ELISA and LFRT assays were more sensitive for primary than secondary dengue, and for specimens collected within 3 days of illness onset relative to later time points. The presence of measurable DENV-reactive IgG and to a lesser extent IgM in the test sample was associated with a significantly lower rate of NS1 detection in both assays. NS1 positivity was associated with the underlying viraemia, as NS1-positive samples had a significantly higher viraemia than NS1-negative samples matched for duration of illness. The Platelia and NS1 LFRT were 100% specific, being negative in all febrile patients without evidence of recent dengue, as well as in patients with enteric fever, malaria, Japanese encephalitis and leptospirosis. CONCLUSIONS/SIGNIFICANCE: Collectively, these data suggest NS1 assays deserve inclusion in the diagnostic evaluation of dengue patients, but with due consideration for the limitations in patients who present late in their illness or have a concomitant humoral immune response. |
format | Text |
id | pubmed-2614471 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-26144712009-01-20 Diagnostic Accuracy of NS1 ELISA and Lateral Flow Rapid Tests for Dengue Sensitivity, Specificity and Relationship to Viraemia and Antibody Responses Ty Hang, Vu Minh Nguyet, Nguyen The Trung, Dinh Tricou, Vianney Yoksan, Sutee Minh Dung, Nguyen Van Ngoc, Tran Hien, Tran Tinh Farrar, Jeremy Wills, Bridget Simmons, Cameron P. PLoS Negl Trop Dis Research Article BACKGROUND: Dengue is a public health problem in many countries. Rapid diagnosis of dengue can assist patient triage and management. Detection of the dengue viral protein, NS1, represents a new approach to dengue diagnosis. METHODOLOGY/PRINCIPAL FINDINGS: The sensitivity and specificity of the Platelia NS1 ELISA assay and an NS1 lateral flow rapid test (LFRT) were compared against a gold standard reference diagnostic algorithm in 138 Vietnamese children and adults. Overall, the Platelia NS1 ELISA was modestly more sensitive (82%) than the NS1 LFRT (72%) in confirmed dengue cases. Both ELISA and LFRT assays were more sensitive for primary than secondary dengue, and for specimens collected within 3 days of illness onset relative to later time points. The presence of measurable DENV-reactive IgG and to a lesser extent IgM in the test sample was associated with a significantly lower rate of NS1 detection in both assays. NS1 positivity was associated with the underlying viraemia, as NS1-positive samples had a significantly higher viraemia than NS1-negative samples matched for duration of illness. The Platelia and NS1 LFRT were 100% specific, being negative in all febrile patients without evidence of recent dengue, as well as in patients with enteric fever, malaria, Japanese encephalitis and leptospirosis. CONCLUSIONS/SIGNIFICANCE: Collectively, these data suggest NS1 assays deserve inclusion in the diagnostic evaluation of dengue patients, but with due consideration for the limitations in patients who present late in their illness or have a concomitant humoral immune response. Public Library of Science 2009-01-20 /pmc/articles/PMC2614471/ /pubmed/19156192 http://dx.doi.org/10.1371/journal.pntd.0000360 Text en Ty Hang et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Ty Hang, Vu Minh Nguyet, Nguyen The Trung, Dinh Tricou, Vianney Yoksan, Sutee Minh Dung, Nguyen Van Ngoc, Tran Hien, Tran Tinh Farrar, Jeremy Wills, Bridget Simmons, Cameron P. Diagnostic Accuracy of NS1 ELISA and Lateral Flow Rapid Tests for Dengue Sensitivity, Specificity and Relationship to Viraemia and Antibody Responses |
title | Diagnostic Accuracy of NS1 ELISA and Lateral Flow Rapid Tests for Dengue Sensitivity, Specificity and Relationship to Viraemia and Antibody Responses |
title_full | Diagnostic Accuracy of NS1 ELISA and Lateral Flow Rapid Tests for Dengue Sensitivity, Specificity and Relationship to Viraemia and Antibody Responses |
title_fullStr | Diagnostic Accuracy of NS1 ELISA and Lateral Flow Rapid Tests for Dengue Sensitivity, Specificity and Relationship to Viraemia and Antibody Responses |
title_full_unstemmed | Diagnostic Accuracy of NS1 ELISA and Lateral Flow Rapid Tests for Dengue Sensitivity, Specificity and Relationship to Viraemia and Antibody Responses |
title_short | Diagnostic Accuracy of NS1 ELISA and Lateral Flow Rapid Tests for Dengue Sensitivity, Specificity and Relationship to Viraemia and Antibody Responses |
title_sort | diagnostic accuracy of ns1 elisa and lateral flow rapid tests for dengue sensitivity, specificity and relationship to viraemia and antibody responses |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2614471/ https://www.ncbi.nlm.nih.gov/pubmed/19156192 http://dx.doi.org/10.1371/journal.pntd.0000360 |
work_keys_str_mv | AT tyhangvu diagnosticaccuracyofns1elisaandlateralflowrapidtestsfordenguesensitivityspecificityandrelationshiptoviraemiaandantibodyresponses AT minhnguyetnguyen diagnosticaccuracyofns1elisaandlateralflowrapidtestsfordenguesensitivityspecificityandrelationshiptoviraemiaandantibodyresponses AT thetrungdinh diagnosticaccuracyofns1elisaandlateralflowrapidtestsfordenguesensitivityspecificityandrelationshiptoviraemiaandantibodyresponses AT tricouvianney diagnosticaccuracyofns1elisaandlateralflowrapidtestsfordenguesensitivityspecificityandrelationshiptoviraemiaandantibodyresponses AT yoksansutee diagnosticaccuracyofns1elisaandlateralflowrapidtestsfordenguesensitivityspecificityandrelationshiptoviraemiaandantibodyresponses AT minhdungnguyen diagnosticaccuracyofns1elisaandlateralflowrapidtestsfordenguesensitivityspecificityandrelationshiptoviraemiaandantibodyresponses AT vanngoctran diagnosticaccuracyofns1elisaandlateralflowrapidtestsfordenguesensitivityspecificityandrelationshiptoviraemiaandantibodyresponses AT hientrantinh diagnosticaccuracyofns1elisaandlateralflowrapidtestsfordenguesensitivityspecificityandrelationshiptoviraemiaandantibodyresponses AT farrarjeremy diagnosticaccuracyofns1elisaandlateralflowrapidtestsfordenguesensitivityspecificityandrelationshiptoviraemiaandantibodyresponses AT willsbridget diagnosticaccuracyofns1elisaandlateralflowrapidtestsfordenguesensitivityspecificityandrelationshiptoviraemiaandantibodyresponses AT simmonscameronp diagnosticaccuracyofns1elisaandlateralflowrapidtestsfordenguesensitivityspecificityandrelationshiptoviraemiaandantibodyresponses |