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Behavior of osteoblastic cells cultured on titanium and structured zirconia surfaces

BACKGROUND: Osseointegration is crucial for the long-term success of dental implants and depends on the tissue reaction at the tissue-implant interface. Mechanical properties and biocompatibility make zirconia a suitable material for dental implants, although surface processings are still problemati...

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Autores principales: Depprich, Rita, Ommerborn, Michelle, Zipprich, Holger, Naujoks, Christian, Handschel, Jörg, Wiesmann, Hans-Peter, Kübler, Norbert R, Meyer, Ulrich
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2614982/
https://www.ncbi.nlm.nih.gov/pubmed/19063728
http://dx.doi.org/10.1186/1746-160X-4-29
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author Depprich, Rita
Ommerborn, Michelle
Zipprich, Holger
Naujoks, Christian
Handschel, Jörg
Wiesmann, Hans-Peter
Kübler, Norbert R
Meyer, Ulrich
author_facet Depprich, Rita
Ommerborn, Michelle
Zipprich, Holger
Naujoks, Christian
Handschel, Jörg
Wiesmann, Hans-Peter
Kübler, Norbert R
Meyer, Ulrich
author_sort Depprich, Rita
collection PubMed
description BACKGROUND: Osseointegration is crucial for the long-term success of dental implants and depends on the tissue reaction at the tissue-implant interface. Mechanical properties and biocompatibility make zirconia a suitable material for dental implants, although surface processings are still problematic. The aim of the present study was to compare osteoblast behavior on structured zirconia and titanium surfaces under standardized conditions. METHODS: The surface characteristics were determined by scanning electron microscopy (SEM). In primary bovine osteoblasts attachment kinetics, proliferation rate and synthesis of bone-associated proteins were tested on different surfaces. RESULTS: The results demonstrated that the proliferation rate of cells was significantly higher on zirconia surfaces than on titanium surfaces (p < 0.05; Student's t-test). In contrast, attachment and adhesion strength of the primary cells was significant higher on titanium surfaces (p < 0.05; U test). No significant differences were found in the synthesis of bone-specific proteins. Ultrastructural analysis revealed phenotypic features of osteoblast-like cells on both zirconia and titanium surfaces. CONCLUSION: The study demonstrates distinct effects of the surface composition on osteoblasts in culture. Zirconia improves cell proliferation significantly during the first days of culture, but it does not improve attachment and adhesion strength. Both materials do not differ with respect to protein synthesis or ultrastructural appearance of osteoblasts. Zirconium oxide may therefore be a suitable material for dental implants.
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spelling pubmed-26149822009-01-08 Behavior of osteoblastic cells cultured on titanium and structured zirconia surfaces Depprich, Rita Ommerborn, Michelle Zipprich, Holger Naujoks, Christian Handschel, Jörg Wiesmann, Hans-Peter Kübler, Norbert R Meyer, Ulrich Head Face Med Research BACKGROUND: Osseointegration is crucial for the long-term success of dental implants and depends on the tissue reaction at the tissue-implant interface. Mechanical properties and biocompatibility make zirconia a suitable material for dental implants, although surface processings are still problematic. The aim of the present study was to compare osteoblast behavior on structured zirconia and titanium surfaces under standardized conditions. METHODS: The surface characteristics were determined by scanning electron microscopy (SEM). In primary bovine osteoblasts attachment kinetics, proliferation rate and synthesis of bone-associated proteins were tested on different surfaces. RESULTS: The results demonstrated that the proliferation rate of cells was significantly higher on zirconia surfaces than on titanium surfaces (p < 0.05; Student's t-test). In contrast, attachment and adhesion strength of the primary cells was significant higher on titanium surfaces (p < 0.05; U test). No significant differences were found in the synthesis of bone-specific proteins. Ultrastructural analysis revealed phenotypic features of osteoblast-like cells on both zirconia and titanium surfaces. CONCLUSION: The study demonstrates distinct effects of the surface composition on osteoblasts in culture. Zirconia improves cell proliferation significantly during the first days of culture, but it does not improve attachment and adhesion strength. Both materials do not differ with respect to protein synthesis or ultrastructural appearance of osteoblasts. Zirconium oxide may therefore be a suitable material for dental implants. BioMed Central 2008-12-08 /pmc/articles/PMC2614982/ /pubmed/19063728 http://dx.doi.org/10.1186/1746-160X-4-29 Text en Copyright © 2008 Depprich et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Depprich, Rita
Ommerborn, Michelle
Zipprich, Holger
Naujoks, Christian
Handschel, Jörg
Wiesmann, Hans-Peter
Kübler, Norbert R
Meyer, Ulrich
Behavior of osteoblastic cells cultured on titanium and structured zirconia surfaces
title Behavior of osteoblastic cells cultured on titanium and structured zirconia surfaces
title_full Behavior of osteoblastic cells cultured on titanium and structured zirconia surfaces
title_fullStr Behavior of osteoblastic cells cultured on titanium and structured zirconia surfaces
title_full_unstemmed Behavior of osteoblastic cells cultured on titanium and structured zirconia surfaces
title_short Behavior of osteoblastic cells cultured on titanium and structured zirconia surfaces
title_sort behavior of osteoblastic cells cultured on titanium and structured zirconia surfaces
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2614982/
https://www.ncbi.nlm.nih.gov/pubmed/19063728
http://dx.doi.org/10.1186/1746-160X-4-29
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