Cargando…

Reduced diversity and increased virulence-gene carriage in intestinal enterobacteria of coeliac children

BACKGROUND: Coeliac disease is an immune-mediated enteropathology triggered by the ingestion of cereal gluten proteins. This disorder is associated with imbalances in the composition of the gut microbiota that could be involved in its pathogenesis. The aim of the present study was to determine wheth...

Descripción completa

Detalles Bibliográficos
Autores principales: Sánchez, Ester, Nadal, Inmaculada, Donat, Ester, Ribes-Koninckx, Carmen, Calabuig, Miguel, Sanz, Yolanda
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2615025/
https://www.ncbi.nlm.nih.gov/pubmed/18983674
http://dx.doi.org/10.1186/1471-230X-8-50
_version_ 1782163276000395264
author Sánchez, Ester
Nadal, Inmaculada
Donat, Ester
Ribes-Koninckx, Carmen
Calabuig, Miguel
Sanz, Yolanda
author_facet Sánchez, Ester
Nadal, Inmaculada
Donat, Ester
Ribes-Koninckx, Carmen
Calabuig, Miguel
Sanz, Yolanda
author_sort Sánchez, Ester
collection PubMed
description BACKGROUND: Coeliac disease is an immune-mediated enteropathology triggered by the ingestion of cereal gluten proteins. This disorder is associated with imbalances in the composition of the gut microbiota that could be involved in its pathogenesis. The aim of the present study was to determine whether intestinal Enterobacteriaceae populations of active and non-active coeliac patients and healthy children differ in diversity and virulence-gene carriage, so as to establish a possible link between the pathogenic potential of enterobacteria and the disease. METHODS: Enterobacteriaceae clones were isolated on VRBD agar from faecal samples of 31 subjects (10 active coeliac patients, 10 symptom-free coeliac patients and 11 healthy controls) and identified at species level by the API 20E system. Escherichia coli clones were classified into four phylogenetic groups A, B1, B2 and D and the prevalence of eight virulence-associated genes (type-1 fimbriae [fimA], P fimbriae [papC], S fimbriae [sfaD/E], Dr haemagglutinin [draA], haemolysin [hlyA], capsule K1 [neuB], capsule K5 [KfiC] and aerobactin [iutA]) was determined by multiplex PCR. RESULTS: A total of 155 Enterobacteriaceae clones were isolated. Non-E. coli clones were more commonly isolated in healthy children than in coeliac patients. The four phylogenetic E. coli groups were equally distributed in healthy children, while in both coeliac patients most commensal isolates belonged to group A. Within the virulent groups, B2 was the most prevalent in active coeliac disease children, while D was the most prevalent in non-active coeliac patients. E coli clones of the virulent phylogenetic groups (B2+D) from active and non-active coeliac patients carried a higher number of virulence genes than those from healthy individuals. Prevalence of P fimbriae (papC), capsule K5 (sfaD/E) and haemolysin (hlyA) genes was higher in E. coli isolated from active and non-active coeliac children than in those from control subjects. CONCLUSION: This study has demonstrated that virulence features of the enteric microbiota are linked to coeliac disease.
format Text
id pubmed-2615025
institution National Center for Biotechnology Information
language English
publishDate 2008
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-26150252009-01-08 Reduced diversity and increased virulence-gene carriage in intestinal enterobacteria of coeliac children Sánchez, Ester Nadal, Inmaculada Donat, Ester Ribes-Koninckx, Carmen Calabuig, Miguel Sanz, Yolanda BMC Gastroenterol Research Article BACKGROUND: Coeliac disease is an immune-mediated enteropathology triggered by the ingestion of cereal gluten proteins. This disorder is associated with imbalances in the composition of the gut microbiota that could be involved in its pathogenesis. The aim of the present study was to determine whether intestinal Enterobacteriaceae populations of active and non-active coeliac patients and healthy children differ in diversity and virulence-gene carriage, so as to establish a possible link between the pathogenic potential of enterobacteria and the disease. METHODS: Enterobacteriaceae clones were isolated on VRBD agar from faecal samples of 31 subjects (10 active coeliac patients, 10 symptom-free coeliac patients and 11 healthy controls) and identified at species level by the API 20E system. Escherichia coli clones were classified into four phylogenetic groups A, B1, B2 and D and the prevalence of eight virulence-associated genes (type-1 fimbriae [fimA], P fimbriae [papC], S fimbriae [sfaD/E], Dr haemagglutinin [draA], haemolysin [hlyA], capsule K1 [neuB], capsule K5 [KfiC] and aerobactin [iutA]) was determined by multiplex PCR. RESULTS: A total of 155 Enterobacteriaceae clones were isolated. Non-E. coli clones were more commonly isolated in healthy children than in coeliac patients. The four phylogenetic E. coli groups were equally distributed in healthy children, while in both coeliac patients most commensal isolates belonged to group A. Within the virulent groups, B2 was the most prevalent in active coeliac disease children, while D was the most prevalent in non-active coeliac patients. E coli clones of the virulent phylogenetic groups (B2+D) from active and non-active coeliac patients carried a higher number of virulence genes than those from healthy individuals. Prevalence of P fimbriae (papC), capsule K5 (sfaD/E) and haemolysin (hlyA) genes was higher in E. coli isolated from active and non-active coeliac children than in those from control subjects. CONCLUSION: This study has demonstrated that virulence features of the enteric microbiota are linked to coeliac disease. BioMed Central 2008-11-04 /pmc/articles/PMC2615025/ /pubmed/18983674 http://dx.doi.org/10.1186/1471-230X-8-50 Text en Copyright © 2008 Sánchez et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Sánchez, Ester
Nadal, Inmaculada
Donat, Ester
Ribes-Koninckx, Carmen
Calabuig, Miguel
Sanz, Yolanda
Reduced diversity and increased virulence-gene carriage in intestinal enterobacteria of coeliac children
title Reduced diversity and increased virulence-gene carriage in intestinal enterobacteria of coeliac children
title_full Reduced diversity and increased virulence-gene carriage in intestinal enterobacteria of coeliac children
title_fullStr Reduced diversity and increased virulence-gene carriage in intestinal enterobacteria of coeliac children
title_full_unstemmed Reduced diversity and increased virulence-gene carriage in intestinal enterobacteria of coeliac children
title_short Reduced diversity and increased virulence-gene carriage in intestinal enterobacteria of coeliac children
title_sort reduced diversity and increased virulence-gene carriage in intestinal enterobacteria of coeliac children
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2615025/
https://www.ncbi.nlm.nih.gov/pubmed/18983674
http://dx.doi.org/10.1186/1471-230X-8-50
work_keys_str_mv AT sanchezester reduceddiversityandincreasedvirulencegenecarriageinintestinalenterobacteriaofcoeliacchildren
AT nadalinmaculada reduceddiversityandincreasedvirulencegenecarriageinintestinalenterobacteriaofcoeliacchildren
AT donatester reduceddiversityandincreasedvirulencegenecarriageinintestinalenterobacteriaofcoeliacchildren
AT ribeskoninckxcarmen reduceddiversityandincreasedvirulencegenecarriageinintestinalenterobacteriaofcoeliacchildren
AT calabuigmiguel reduceddiversityandincreasedvirulencegenecarriageinintestinalenterobacteriaofcoeliacchildren
AT sanzyolanda reduceddiversityandincreasedvirulencegenecarriageinintestinalenterobacteriaofcoeliacchildren