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Two evolutionarily conserved sequence elements for Peg3/Usp29 transcription
BACKGROUND: Two evolutionarily Conserved Sequence Elements, CSE1 and CSE2 (YY1 binding sites), are found within the 3.8-kb CpG island surrounding the bidirectional promoter of two imprinted genes, Peg3 (Paternally expressed gene 3) and Usp29 (Ubiquitin-specific protease 29). This CpG island is a lik...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2615030/ https://www.ncbi.nlm.nih.gov/pubmed/19068137 http://dx.doi.org/10.1186/1471-2199-9-108 |
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author | Kim, Jeong Do Yu, Sungryul Choo, Jung Ha Kim, Joomyeong |
author_facet | Kim, Jeong Do Yu, Sungryul Choo, Jung Ha Kim, Joomyeong |
author_sort | Kim, Jeong Do |
collection | PubMed |
description | BACKGROUND: Two evolutionarily Conserved Sequence Elements, CSE1 and CSE2 (YY1 binding sites), are found within the 3.8-kb CpG island surrounding the bidirectional promoter of two imprinted genes, Peg3 (Paternally expressed gene 3) and Usp29 (Ubiquitin-specific protease 29). This CpG island is a likely ICR (Imprinting Control Region) that controls transcription of the 500-kb genomic region of the Peg3 imprinted domain. RESULTS: The current study investigated the functional roles of CSE1 and CSE2 in the transcriptional control of the two genes, Peg3 and Usp29, using cell line-based promoter assays. The mutation of 6 YY1 binding sites (CSE2) reduced the transcriptional activity of the bidirectional promoter in the Peg3 direction in an orientation-dependent manner, suggesting an activator role for CSE2 (YY1 binding sites). However, the activity in the Usp29 direction was not detectable regardless of the presence/absence of YY1 binding sites. In contrast, mutation of CSE1 increased the transcriptional activity of the promoter in both the Peg3 and Usp29 directions, suggesting a potential repressor role for CSE1. The observed repression by CSE1 was also orientation-dependent. Serial mutational analyses further narrowed down two separate 6-bp-long regions within the 42-bp-long CSE1 which are individually responsible for the repression of Peg3 and Usp29. CONCLUSION: CSE2 (YY1 binding sites) functions as an activator for Peg3 transcription, while CSE1 acts as a repressor for the transcription of both Peg3 and Usp29. |
format | Text |
id | pubmed-2615030 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-26150302009-01-08 Two evolutionarily conserved sequence elements for Peg3/Usp29 transcription Kim, Jeong Do Yu, Sungryul Choo, Jung Ha Kim, Joomyeong BMC Mol Biol Research Article BACKGROUND: Two evolutionarily Conserved Sequence Elements, CSE1 and CSE2 (YY1 binding sites), are found within the 3.8-kb CpG island surrounding the bidirectional promoter of two imprinted genes, Peg3 (Paternally expressed gene 3) and Usp29 (Ubiquitin-specific protease 29). This CpG island is a likely ICR (Imprinting Control Region) that controls transcription of the 500-kb genomic region of the Peg3 imprinted domain. RESULTS: The current study investigated the functional roles of CSE1 and CSE2 in the transcriptional control of the two genes, Peg3 and Usp29, using cell line-based promoter assays. The mutation of 6 YY1 binding sites (CSE2) reduced the transcriptional activity of the bidirectional promoter in the Peg3 direction in an orientation-dependent manner, suggesting an activator role for CSE2 (YY1 binding sites). However, the activity in the Usp29 direction was not detectable regardless of the presence/absence of YY1 binding sites. In contrast, mutation of CSE1 increased the transcriptional activity of the promoter in both the Peg3 and Usp29 directions, suggesting a potential repressor role for CSE1. The observed repression by CSE1 was also orientation-dependent. Serial mutational analyses further narrowed down two separate 6-bp-long regions within the 42-bp-long CSE1 which are individually responsible for the repression of Peg3 and Usp29. CONCLUSION: CSE2 (YY1 binding sites) functions as an activator for Peg3 transcription, while CSE1 acts as a repressor for the transcription of both Peg3 and Usp29. BioMed Central 2008-12-10 /pmc/articles/PMC2615030/ /pubmed/19068137 http://dx.doi.org/10.1186/1471-2199-9-108 Text en Copyright © 2008 Kim et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Kim, Jeong Do Yu, Sungryul Choo, Jung Ha Kim, Joomyeong Two evolutionarily conserved sequence elements for Peg3/Usp29 transcription |
title | Two evolutionarily conserved sequence elements for Peg3/Usp29 transcription |
title_full | Two evolutionarily conserved sequence elements for Peg3/Usp29 transcription |
title_fullStr | Two evolutionarily conserved sequence elements for Peg3/Usp29 transcription |
title_full_unstemmed | Two evolutionarily conserved sequence elements for Peg3/Usp29 transcription |
title_short | Two evolutionarily conserved sequence elements for Peg3/Usp29 transcription |
title_sort | two evolutionarily conserved sequence elements for peg3/usp29 transcription |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2615030/ https://www.ncbi.nlm.nih.gov/pubmed/19068137 http://dx.doi.org/10.1186/1471-2199-9-108 |
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