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Tim23–Tim50 pair coordinates functions of translocators and motor proteins in mitochondrial protein import
Mitochondrial protein traffic requires coordinated operation of protein translocator complexes in the mitochondrial membrane. The TIM23 complex translocates and inserts proteins into the mitochondrial inner membrane. Here we analyze the intermembrane space (IMS) domains of Tim23 and Tim50, which are...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2615085/ https://www.ncbi.nlm.nih.gov/pubmed/19139266 http://dx.doi.org/10.1083/jcb.200808068 |
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author | Tamura, Yasushi Harada, Yoshihiro Shiota, Takuya Yamano, Koji Watanabe, Kazuaki Yokota, Mihoko Yamamoto, Hayashi Sesaki, Hiromi Endo, Toshiya |
author_facet | Tamura, Yasushi Harada, Yoshihiro Shiota, Takuya Yamano, Koji Watanabe, Kazuaki Yokota, Mihoko Yamamoto, Hayashi Sesaki, Hiromi Endo, Toshiya |
author_sort | Tamura, Yasushi |
collection | PubMed |
description | Mitochondrial protein traffic requires coordinated operation of protein translocator complexes in the mitochondrial membrane. The TIM23 complex translocates and inserts proteins into the mitochondrial inner membrane. Here we analyze the intermembrane space (IMS) domains of Tim23 and Tim50, which are essential subunits of the TIM23 complex, in these functions. We find that interactions of Tim23 and Tim50 in the IMS facilitate transfer of precursor proteins from the TOM40 complex, a general protein translocator in the outer membrane, to the TIM23 complex. Tim23–Tim50 interactions also facilitate a late step of protein translocation across the inner membrane by promoting motor functions of mitochondrial Hsp70 in the matrix. Therefore, the Tim23–Tim50 pair coordinates the actions of the TOM40 and TIM23 complexes together with motor proteins for mitochondrial protein import. |
format | Text |
id | pubmed-2615085 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-26150852009-07-12 Tim23–Tim50 pair coordinates functions of translocators and motor proteins in mitochondrial protein import Tamura, Yasushi Harada, Yoshihiro Shiota, Takuya Yamano, Koji Watanabe, Kazuaki Yokota, Mihoko Yamamoto, Hayashi Sesaki, Hiromi Endo, Toshiya J Cell Biol Research Articles Mitochondrial protein traffic requires coordinated operation of protein translocator complexes in the mitochondrial membrane. The TIM23 complex translocates and inserts proteins into the mitochondrial inner membrane. Here we analyze the intermembrane space (IMS) domains of Tim23 and Tim50, which are essential subunits of the TIM23 complex, in these functions. We find that interactions of Tim23 and Tim50 in the IMS facilitate transfer of precursor proteins from the TOM40 complex, a general protein translocator in the outer membrane, to the TIM23 complex. Tim23–Tim50 interactions also facilitate a late step of protein translocation across the inner membrane by promoting motor functions of mitochondrial Hsp70 in the matrix. Therefore, the Tim23–Tim50 pair coordinates the actions of the TOM40 and TIM23 complexes together with motor proteins for mitochondrial protein import. The Rockefeller University Press 2009-01-12 /pmc/articles/PMC2615085/ /pubmed/19139266 http://dx.doi.org/10.1083/jcb.200808068 Text en © 2009 Tamura et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Tamura, Yasushi Harada, Yoshihiro Shiota, Takuya Yamano, Koji Watanabe, Kazuaki Yokota, Mihoko Yamamoto, Hayashi Sesaki, Hiromi Endo, Toshiya Tim23–Tim50 pair coordinates functions of translocators and motor proteins in mitochondrial protein import |
title | Tim23–Tim50 pair coordinates functions of translocators and motor proteins in mitochondrial protein import |
title_full | Tim23–Tim50 pair coordinates functions of translocators and motor proteins in mitochondrial protein import |
title_fullStr | Tim23–Tim50 pair coordinates functions of translocators and motor proteins in mitochondrial protein import |
title_full_unstemmed | Tim23–Tim50 pair coordinates functions of translocators and motor proteins in mitochondrial protein import |
title_short | Tim23–Tim50 pair coordinates functions of translocators and motor proteins in mitochondrial protein import |
title_sort | tim23–tim50 pair coordinates functions of translocators and motor proteins in mitochondrial protein import |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2615085/ https://www.ncbi.nlm.nih.gov/pubmed/19139266 http://dx.doi.org/10.1083/jcb.200808068 |
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