A role for NANOG in G1 to S transition in human embryonic stem cells through direct binding of CDK6 and CDC25A

In this study, we show that NANOG, a master transcription factor, regulates S-phase entry in human embryonic stem cells (hESCs) via transcriptional regulation of cell cycle regulatory components. Chromatin immunoprecipitation combined with reporter-based transfection assays show that the C-terminal...

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Autores principales: Zhang, Xin, Neganova, Irina, Przyborski, Stefan, Yang, Chunbo, Cooke, Michael, Atkinson, Stuart P., Anyfantis, George, Fenyk, Stefan, Keith, W. Nicol, Hoare, Stacey F., Hughes, Owen, Strachan, Tom, Stojkovic, Miodrag, Hinds, Philip W., Armstrong, Lyle, Lako, Majlinda
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2009
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2615089/
https://www.ncbi.nlm.nih.gov/pubmed/19139263
http://dx.doi.org/10.1083/jcb.200801009
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author Zhang, Xin
Neganova, Irina
Przyborski, Stefan
Yang, Chunbo
Cooke, Michael
Atkinson, Stuart P.
Anyfantis, George
Fenyk, Stefan
Keith, W. Nicol
Hoare, Stacey F.
Hughes, Owen
Strachan, Tom
Stojkovic, Miodrag
Hinds, Philip W.
Armstrong, Lyle
Lako, Majlinda
author_facet Zhang, Xin
Neganova, Irina
Przyborski, Stefan
Yang, Chunbo
Cooke, Michael
Atkinson, Stuart P.
Anyfantis, George
Fenyk, Stefan
Keith, W. Nicol
Hoare, Stacey F.
Hughes, Owen
Strachan, Tom
Stojkovic, Miodrag
Hinds, Philip W.
Armstrong, Lyle
Lako, Majlinda
author_sort Zhang, Xin
collection PubMed
description In this study, we show that NANOG, a master transcription factor, regulates S-phase entry in human embryonic stem cells (hESCs) via transcriptional regulation of cell cycle regulatory components. Chromatin immunoprecipitation combined with reporter-based transfection assays show that the C-terminal region of NANOG binds to the regulatory regions of CDK6 and CDC25A genes under normal physiological conditions. Decreased CDK6 and CDC25A expression in hESCs suggest that both CDK6 and CDC25A are involved in S-phase regulation. The effects of NANOG overexpression on S-phase regulation are mitigated by the down-regulation of CDK6 or CDC25A alone. Overexpression of CDK6 or CDC25A alone can rescue the impact of NANOG down-regulation on S-phase entry, suggesting that CDK6 and CDC25A are downstream cell cycle effectors of NANOG during the G1 to S transition.
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spelling pubmed-26150892009-07-12 A role for NANOG in G1 to S transition in human embryonic stem cells through direct binding of CDK6 and CDC25A Zhang, Xin Neganova, Irina Przyborski, Stefan Yang, Chunbo Cooke, Michael Atkinson, Stuart P. Anyfantis, George Fenyk, Stefan Keith, W. Nicol Hoare, Stacey F. Hughes, Owen Strachan, Tom Stojkovic, Miodrag Hinds, Philip W. Armstrong, Lyle Lako, Majlinda J Cell Biol Research Articles In this study, we show that NANOG, a master transcription factor, regulates S-phase entry in human embryonic stem cells (hESCs) via transcriptional regulation of cell cycle regulatory components. Chromatin immunoprecipitation combined with reporter-based transfection assays show that the C-terminal region of NANOG binds to the regulatory regions of CDK6 and CDC25A genes under normal physiological conditions. Decreased CDK6 and CDC25A expression in hESCs suggest that both CDK6 and CDC25A are involved in S-phase regulation. The effects of NANOG overexpression on S-phase regulation are mitigated by the down-regulation of CDK6 or CDC25A alone. Overexpression of CDK6 or CDC25A alone can rescue the impact of NANOG down-regulation on S-phase entry, suggesting that CDK6 and CDC25A are downstream cell cycle effectors of NANOG during the G1 to S transition. The Rockefeller University Press 2009-01-12 /pmc/articles/PMC2615089/ /pubmed/19139263 http://dx.doi.org/10.1083/jcb.200801009 Text en © 2009 Zhang et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Zhang, Xin
Neganova, Irina
Przyborski, Stefan
Yang, Chunbo
Cooke, Michael
Atkinson, Stuart P.
Anyfantis, George
Fenyk, Stefan
Keith, W. Nicol
Hoare, Stacey F.
Hughes, Owen
Strachan, Tom
Stojkovic, Miodrag
Hinds, Philip W.
Armstrong, Lyle
Lako, Majlinda
A role for NANOG in G1 to S transition in human embryonic stem cells through direct binding of CDK6 and CDC25A
title A role for NANOG in G1 to S transition in human embryonic stem cells through direct binding of CDK6 and CDC25A
title_full A role for NANOG in G1 to S transition in human embryonic stem cells through direct binding of CDK6 and CDC25A
title_fullStr A role for NANOG in G1 to S transition in human embryonic stem cells through direct binding of CDK6 and CDC25A
title_full_unstemmed A role for NANOG in G1 to S transition in human embryonic stem cells through direct binding of CDK6 and CDC25A
title_short A role for NANOG in G1 to S transition in human embryonic stem cells through direct binding of CDK6 and CDC25A
title_sort role for nanog in g1 to s transition in human embryonic stem cells through direct binding of cdk6 and cdc25a
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2615089/
https://www.ncbi.nlm.nih.gov/pubmed/19139263
http://dx.doi.org/10.1083/jcb.200801009
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