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Targeted deletion of SAP1 abolishes the expression of infectivity factors necessary for successful malaria parasite liver infection

Malaria parasite sporozoites prepare for transmission to a mammalian host by upregulation of UIS (Upregulated in Infectious Sporozoites) genes. A number of UIS gene products are essential for the establishment of the intrahepatocytic niche. However, the factors that regulate the expression of genes...

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Autores principales: Aly, Ahmed S I, Mikolajczak, Sebastian A, Rivera, Hilda Silva, Camargo, Nelly, Jacobs-Lorena, Vanessa, Labaied, Mehdi, Coppens, Isabelle, Kappe, Stefan H I
Formato: Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2615191/
https://www.ncbi.nlm.nih.gov/pubmed/18466298
http://dx.doi.org/10.1111/j.1365-2958.2008.06271.x
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author Aly, Ahmed S I
Mikolajczak, Sebastian A
Rivera, Hilda Silva
Camargo, Nelly
Jacobs-Lorena, Vanessa
Labaied, Mehdi
Coppens, Isabelle
Kappe, Stefan H I
author_facet Aly, Ahmed S I
Mikolajczak, Sebastian A
Rivera, Hilda Silva
Camargo, Nelly
Jacobs-Lorena, Vanessa
Labaied, Mehdi
Coppens, Isabelle
Kappe, Stefan H I
author_sort Aly, Ahmed S I
collection PubMed
description Malaria parasite sporozoites prepare for transmission to a mammalian host by upregulation of UIS (Upregulated in Infectious Sporozoites) genes. A number of UIS gene products are essential for the establishment of the intrahepatocytic niche. However, the factors that regulate the expression of genes involved in gain of infectivity for the liver are unknown. Herein, we show that a conserved Plasmodium sporozoite low-complexity asparagine-rich protein, SAP1 (Sporozoite Asparagine-rich Protein 1), has an essential role in malaria parasite liver infection. Targeted deletion of SAP1 in the rodent malaria parasite Plasmodium yoelii generated mutant parasites that traverse and invade hepatocytes normally but cannot initiate liver-stage development in vitro and in vivo. Moreover, immunizations with Pysap1(−) sporozoites confer long-lasting sterile protection against wild-type sporozoite infection. Strikingly, lack of SAP1 abolished expression of essential UIS genes including UIS3, UIS4 and P52 but not the constitutively expressed genes encoding, among others, sporozoite proteins CSP and TRAP. SAP1 localization to the cell interior but not the nucleus of sporozoites suggests its involvement in a post-transcriptional mechanism of gene expression control. These findings demonstrate that SAP1 is essential for liver infection possibly by functioning as a selective regulator controlling the expression of infectivity-associated parasite effector genes.
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spelling pubmed-26151912009-01-12 Targeted deletion of SAP1 abolishes the expression of infectivity factors necessary for successful malaria parasite liver infection Aly, Ahmed S I Mikolajczak, Sebastian A Rivera, Hilda Silva Camargo, Nelly Jacobs-Lorena, Vanessa Labaied, Mehdi Coppens, Isabelle Kappe, Stefan H I Mol Microbiol Research Articles Malaria parasite sporozoites prepare for transmission to a mammalian host by upregulation of UIS (Upregulated in Infectious Sporozoites) genes. A number of UIS gene products are essential for the establishment of the intrahepatocytic niche. However, the factors that regulate the expression of genes involved in gain of infectivity for the liver are unknown. Herein, we show that a conserved Plasmodium sporozoite low-complexity asparagine-rich protein, SAP1 (Sporozoite Asparagine-rich Protein 1), has an essential role in malaria parasite liver infection. Targeted deletion of SAP1 in the rodent malaria parasite Plasmodium yoelii generated mutant parasites that traverse and invade hepatocytes normally but cannot initiate liver-stage development in vitro and in vivo. Moreover, immunizations with Pysap1(−) sporozoites confer long-lasting sterile protection against wild-type sporozoite infection. Strikingly, lack of SAP1 abolished expression of essential UIS genes including UIS3, UIS4 and P52 but not the constitutively expressed genes encoding, among others, sporozoite proteins CSP and TRAP. SAP1 localization to the cell interior but not the nucleus of sporozoites suggests its involvement in a post-transcriptional mechanism of gene expression control. These findings demonstrate that SAP1 is essential for liver infection possibly by functioning as a selective regulator controlling the expression of infectivity-associated parasite effector genes. Blackwell Publishing Ltd 2008-07 2008-05-13 /pmc/articles/PMC2615191/ /pubmed/18466298 http://dx.doi.org/10.1111/j.1365-2958.2008.06271.x Text en © 2008 The Authors Journal compilation © 2008 Blackwell Publishing Ltd
spellingShingle Research Articles
Aly, Ahmed S I
Mikolajczak, Sebastian A
Rivera, Hilda Silva
Camargo, Nelly
Jacobs-Lorena, Vanessa
Labaied, Mehdi
Coppens, Isabelle
Kappe, Stefan H I
Targeted deletion of SAP1 abolishes the expression of infectivity factors necessary for successful malaria parasite liver infection
title Targeted deletion of SAP1 abolishes the expression of infectivity factors necessary for successful malaria parasite liver infection
title_full Targeted deletion of SAP1 abolishes the expression of infectivity factors necessary for successful malaria parasite liver infection
title_fullStr Targeted deletion of SAP1 abolishes the expression of infectivity factors necessary for successful malaria parasite liver infection
title_full_unstemmed Targeted deletion of SAP1 abolishes the expression of infectivity factors necessary for successful malaria parasite liver infection
title_short Targeted deletion of SAP1 abolishes the expression of infectivity factors necessary for successful malaria parasite liver infection
title_sort targeted deletion of sap1 abolishes the expression of infectivity factors necessary for successful malaria parasite liver infection
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2615191/
https://www.ncbi.nlm.nih.gov/pubmed/18466298
http://dx.doi.org/10.1111/j.1365-2958.2008.06271.x
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