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PbSR is synthesized in macrogametocytes and involved in formation of the malaria crystalloids

Crystalloids are transient organelles that form in developing malaria ookinetes and disappear after ookinete-to-oocyst transition. Their origins and functions remain poorly understood. The Plasmodium berghei scavenger receptor-like protein PbSR is essential for mosquito-to-host transmission of the p...

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Autores principales: Carter, Victoria, Shimizu, Shoichi, Arai, Meiji, Dessens, Johannes T
Formato: Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2615194/
https://www.ncbi.nlm.nih.gov/pubmed/18452513
http://dx.doi.org/10.1111/j.1365-2958.2008.06254.x
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author Carter, Victoria
Shimizu, Shoichi
Arai, Meiji
Dessens, Johannes T
author_facet Carter, Victoria
Shimizu, Shoichi
Arai, Meiji
Dessens, Johannes T
author_sort Carter, Victoria
collection PubMed
description Crystalloids are transient organelles that form in developing malaria ookinetes and disappear after ookinete-to-oocyst transition. Their origins and functions remain poorly understood. The Plasmodium berghei scavenger receptor-like protein PbSR is essential for mosquito-to-host transmission of the parasite: PbSR knockout parasites produce normal numbers of oocysts that fail to form sporozoites, pointing to a role for PbSR in the oocyst during sporogony. Here, using fluorescent protein tagging and targeted gene disruption, we show that PbSR is synthesized in macrogametocytes, gets targeted to the crystalloids of developing ookinetes and is involved in crystalloid formation. While oocyst sporulation rates of PbSR knockout parasites are highly reduced in parasite-infected mosquitoes, sporulation rates in vitro are not adversely affected, supporting the view that mosquito factors could be involved in the PbSR loss-of-function phenotype. These findings are the first to identify a parasite protein involved with the crystalloid organelle, and suggest a novel protein-trafficking mechanism to deliver PbSR to the oocysts.
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spelling pubmed-26151942009-01-12 PbSR is synthesized in macrogametocytes and involved in formation of the malaria crystalloids Carter, Victoria Shimizu, Shoichi Arai, Meiji Dessens, Johannes T Mol Microbiol Research Articles Crystalloids are transient organelles that form in developing malaria ookinetes and disappear after ookinete-to-oocyst transition. Their origins and functions remain poorly understood. The Plasmodium berghei scavenger receptor-like protein PbSR is essential for mosquito-to-host transmission of the parasite: PbSR knockout parasites produce normal numbers of oocysts that fail to form sporozoites, pointing to a role for PbSR in the oocyst during sporogony. Here, using fluorescent protein tagging and targeted gene disruption, we show that PbSR is synthesized in macrogametocytes, gets targeted to the crystalloids of developing ookinetes and is involved in crystalloid formation. While oocyst sporulation rates of PbSR knockout parasites are highly reduced in parasite-infected mosquitoes, sporulation rates in vitro are not adversely affected, supporting the view that mosquito factors could be involved in the PbSR loss-of-function phenotype. These findings are the first to identify a parasite protein involved with the crystalloid organelle, and suggest a novel protein-trafficking mechanism to deliver PbSR to the oocysts. Blackwell Publishing Ltd 2008-06 2008-04-29 /pmc/articles/PMC2615194/ /pubmed/18452513 http://dx.doi.org/10.1111/j.1365-2958.2008.06254.x Text en © 2008 The Authors Journal compilation © 2008 Blackwell Publishing Ltd
spellingShingle Research Articles
Carter, Victoria
Shimizu, Shoichi
Arai, Meiji
Dessens, Johannes T
PbSR is synthesized in macrogametocytes and involved in formation of the malaria crystalloids
title PbSR is synthesized in macrogametocytes and involved in formation of the malaria crystalloids
title_full PbSR is synthesized in macrogametocytes and involved in formation of the malaria crystalloids
title_fullStr PbSR is synthesized in macrogametocytes and involved in formation of the malaria crystalloids
title_full_unstemmed PbSR is synthesized in macrogametocytes and involved in formation of the malaria crystalloids
title_short PbSR is synthesized in macrogametocytes and involved in formation of the malaria crystalloids
title_sort pbsr is synthesized in macrogametocytes and involved in formation of the malaria crystalloids
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2615194/
https://www.ncbi.nlm.nih.gov/pubmed/18452513
http://dx.doi.org/10.1111/j.1365-2958.2008.06254.x
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