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Mutagenic inverted repeat assisted genome engineering (MIRAGE)
Here we describe a one-step method to create precise modifications in the genome of Saccharomyces cerevisiae as a tool for synthetic biology, metabolic engineering, systems biology and genetic studies. Through homologous recombination, a mutagenesis cassette containing an inverted repeat of selectio...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2009
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2615605/ https://www.ncbi.nlm.nih.gov/pubmed/19050015 http://dx.doi.org/10.1093/nar/gkn943 |