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Gelatinase (MMP-2 and -9) expression profiles during gestation in the bovine endometrium

BACKGROUND: Various molecules participate in implantation and maintaining endometrial function during gestation. The remodeling of endometrial matrices is a necessary process in the coordination of gestational progress. Matrix-metalloproteinases (MMPs) like gelatinases (MMP-2 and -9) and collagenase...

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Autores principales: Kizaki, Keiichiro, Ushizawa, Koichi, Takahashi, Toru, Yamada, Osamu, Todoroki, Junichi, Sato, Takashi, Ito, Akira, Hashizume, Kazuyoshi
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2615774/
https://www.ncbi.nlm.nih.gov/pubmed/19116037
http://dx.doi.org/10.1186/1477-7827-6-66
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author Kizaki, Keiichiro
Ushizawa, Koichi
Takahashi, Toru
Yamada, Osamu
Todoroki, Junichi
Sato, Takashi
Ito, Akira
Hashizume, Kazuyoshi
author_facet Kizaki, Keiichiro
Ushizawa, Koichi
Takahashi, Toru
Yamada, Osamu
Todoroki, Junichi
Sato, Takashi
Ito, Akira
Hashizume, Kazuyoshi
author_sort Kizaki, Keiichiro
collection PubMed
description BACKGROUND: Various molecules participate in implantation and maintaining endometrial function during gestation. The remodeling of endometrial matrices is a necessary process in the coordination of gestational progress. Matrix-metalloproteinases (MMPs) like gelatinases (MMP-2 and -9) and collagenase (MMP-1) are considered to play important roles in this process. We examined MMP-2 and -9 expression using zymography, in situ hybridization, real-time PCR, and microarray analysis to clarify their roles in the bovine endometrium during gestation. METHODS: Endometria, placentomes, and fetal membranes were collected from Japanese black cows that were killed on day 15 to 252 of gestation or during their estrous cycle. The gene expression of MMP-related molecules (mainly MMP-2 and -9) was examined using a custom-made microarray, real-time RT-PCR, and in-situ hybridization. Gelatinase activity was detected by zymography and film in situ zymography. RESULTS: Both gelatinases were expressed in the endometrium and fetal tissues throughout gestation. MMP-2 gene expression declined with the progress of gestation, but its intensity was maintained at a high level during the peri-implantation period and increased in late gestation. The expression level of MMP-9 was stably maintained, but was relatively low compared to that of MMP-2. These gene expression patterns matched those detected by zymography for the proteins. Microarray analysis suggested that the functions of MMP-2 during implantation and the last part of gestation are closely related with those of other molecules such as tissue inhibitors of metalloproteinase (TIMP)-2, a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) 1, membrane type 1 (MT1)-MMP, and extracellular matrix metalloproteinase inducer (EMMPRIN). CONCLUSION: We detected MMP-2 and -9 gene expression in the bovine endometrium and placentome throughout gestation. These data suggest that MMP-2 is one of the main endometrial remodeling factors for implantation and pre-partum in cattle. In cows, as is the case in humans and rodents, gelatinases participate in endometrial remodeling, and their activities depend on the balance of activators and inhibitors; i.e., TIMP, MT-MMP, EMMPRIN, MMP-2, MMP-9, and so on.
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spelling pubmed-26157742009-01-10 Gelatinase (MMP-2 and -9) expression profiles during gestation in the bovine endometrium Kizaki, Keiichiro Ushizawa, Koichi Takahashi, Toru Yamada, Osamu Todoroki, Junichi Sato, Takashi Ito, Akira Hashizume, Kazuyoshi Reprod Biol Endocrinol Research BACKGROUND: Various molecules participate in implantation and maintaining endometrial function during gestation. The remodeling of endometrial matrices is a necessary process in the coordination of gestational progress. Matrix-metalloproteinases (MMPs) like gelatinases (MMP-2 and -9) and collagenase (MMP-1) are considered to play important roles in this process. We examined MMP-2 and -9 expression using zymography, in situ hybridization, real-time PCR, and microarray analysis to clarify their roles in the bovine endometrium during gestation. METHODS: Endometria, placentomes, and fetal membranes were collected from Japanese black cows that were killed on day 15 to 252 of gestation or during their estrous cycle. The gene expression of MMP-related molecules (mainly MMP-2 and -9) was examined using a custom-made microarray, real-time RT-PCR, and in-situ hybridization. Gelatinase activity was detected by zymography and film in situ zymography. RESULTS: Both gelatinases were expressed in the endometrium and fetal tissues throughout gestation. MMP-2 gene expression declined with the progress of gestation, but its intensity was maintained at a high level during the peri-implantation period and increased in late gestation. The expression level of MMP-9 was stably maintained, but was relatively low compared to that of MMP-2. These gene expression patterns matched those detected by zymography for the proteins. Microarray analysis suggested that the functions of MMP-2 during implantation and the last part of gestation are closely related with those of other molecules such as tissue inhibitors of metalloproteinase (TIMP)-2, a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) 1, membrane type 1 (MT1)-MMP, and extracellular matrix metalloproteinase inducer (EMMPRIN). CONCLUSION: We detected MMP-2 and -9 gene expression in the bovine endometrium and placentome throughout gestation. These data suggest that MMP-2 is one of the main endometrial remodeling factors for implantation and pre-partum in cattle. In cows, as is the case in humans and rodents, gelatinases participate in endometrial remodeling, and their activities depend on the balance of activators and inhibitors; i.e., TIMP, MT-MMP, EMMPRIN, MMP-2, MMP-9, and so on. BioMed Central 2008-12-31 /pmc/articles/PMC2615774/ /pubmed/19116037 http://dx.doi.org/10.1186/1477-7827-6-66 Text en Copyright © 2008 Kizaki et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Kizaki, Keiichiro
Ushizawa, Koichi
Takahashi, Toru
Yamada, Osamu
Todoroki, Junichi
Sato, Takashi
Ito, Akira
Hashizume, Kazuyoshi
Gelatinase (MMP-2 and -9) expression profiles during gestation in the bovine endometrium
title Gelatinase (MMP-2 and -9) expression profiles during gestation in the bovine endometrium
title_full Gelatinase (MMP-2 and -9) expression profiles during gestation in the bovine endometrium
title_fullStr Gelatinase (MMP-2 and -9) expression profiles during gestation in the bovine endometrium
title_full_unstemmed Gelatinase (MMP-2 and -9) expression profiles during gestation in the bovine endometrium
title_short Gelatinase (MMP-2 and -9) expression profiles during gestation in the bovine endometrium
title_sort gelatinase (mmp-2 and -9) expression profiles during gestation in the bovine endometrium
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2615774/
https://www.ncbi.nlm.nih.gov/pubmed/19116037
http://dx.doi.org/10.1186/1477-7827-6-66
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