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Viral Discovery and Sequence Recovery Using DNA Microarrays
Because of the constant threat posed by emerging infectious diseases and the limitations of existing approaches used to identify new pathogens, there is a great demand for new technological methods for viral discovery. We describe herein a DNA microarray-based platform for novel virus identification...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2003
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC261870/ https://www.ncbi.nlm.nih.gov/pubmed/14624234 http://dx.doi.org/10.1371/journal.pbio.0000002 |
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author | Wang, David Urisman, Anatoly Liu, Yu-Tsueng Springer, Michael Ksiazek, Thomas G Erdman, Dean D Mardis, Elaine R Hickenbotham, Matthew Magrini, Vincent Eldred, James Latreille, J. Phillipe Wilson, Richard K Ganem, Don DeRisi, Joseph L |
author_facet | Wang, David Urisman, Anatoly Liu, Yu-Tsueng Springer, Michael Ksiazek, Thomas G Erdman, Dean D Mardis, Elaine R Hickenbotham, Matthew Magrini, Vincent Eldred, James Latreille, J. Phillipe Wilson, Richard K Ganem, Don DeRisi, Joseph L |
author_sort | Wang, David |
collection | PubMed |
description | Because of the constant threat posed by emerging infectious diseases and the limitations of existing approaches used to identify new pathogens, there is a great demand for new technological methods for viral discovery. We describe herein a DNA microarray-based platform for novel virus identification and characterization. Central to this approach was a DNA microarray designed to detect a wide range of known viruses as well as novel members of existing viral families; this microarray contained the most highly conserved 70mer sequences from every fully sequenced reference viral genome in GenBank. During an outbreak of severe acute respiratory syndrome (SARS) in March 2003, hybridization to this microarray revealed the presence of a previously uncharacterized coronavirus in a viral isolate cultivated from a SARS patient. To further characterize this new virus, approximately 1 kb of the unknown virus genome was cloned by physically recovering viral sequences hybridized to individual array elements. Sequencing of these fragments confirmed that the virus was indeed a new member of the coronavirus family. This combination of array hybridization followed by direct viral sequence recovery should prove to be a general strategy for the rapid identification and characterization of novel viruses and emerging infectious disease. |
format | Text |
id | pubmed-261870 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2003 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-2618702003-11-17 Viral Discovery and Sequence Recovery Using DNA Microarrays Wang, David Urisman, Anatoly Liu, Yu-Tsueng Springer, Michael Ksiazek, Thomas G Erdman, Dean D Mardis, Elaine R Hickenbotham, Matthew Magrini, Vincent Eldred, James Latreille, J. Phillipe Wilson, Richard K Ganem, Don DeRisi, Joseph L PLoS Biol Research Article Because of the constant threat posed by emerging infectious diseases and the limitations of existing approaches used to identify new pathogens, there is a great demand for new technological methods for viral discovery. We describe herein a DNA microarray-based platform for novel virus identification and characterization. Central to this approach was a DNA microarray designed to detect a wide range of known viruses as well as novel members of existing viral families; this microarray contained the most highly conserved 70mer sequences from every fully sequenced reference viral genome in GenBank. During an outbreak of severe acute respiratory syndrome (SARS) in March 2003, hybridization to this microarray revealed the presence of a previously uncharacterized coronavirus in a viral isolate cultivated from a SARS patient. To further characterize this new virus, approximately 1 kb of the unknown virus genome was cloned by physically recovering viral sequences hybridized to individual array elements. Sequencing of these fragments confirmed that the virus was indeed a new member of the coronavirus family. This combination of array hybridization followed by direct viral sequence recovery should prove to be a general strategy for the rapid identification and characterization of novel viruses and emerging infectious disease. Public Library of Science 2003-11 2003-11-17 /pmc/articles/PMC261870/ /pubmed/14624234 http://dx.doi.org/10.1371/journal.pbio.0000002 Text en Copyright: © 2003 Wang et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Wang, David Urisman, Anatoly Liu, Yu-Tsueng Springer, Michael Ksiazek, Thomas G Erdman, Dean D Mardis, Elaine R Hickenbotham, Matthew Magrini, Vincent Eldred, James Latreille, J. Phillipe Wilson, Richard K Ganem, Don DeRisi, Joseph L Viral Discovery and Sequence Recovery Using DNA Microarrays |
title | Viral Discovery and Sequence Recovery Using DNA Microarrays |
title_full | Viral Discovery and Sequence Recovery Using DNA Microarrays |
title_fullStr | Viral Discovery and Sequence Recovery Using DNA Microarrays |
title_full_unstemmed | Viral Discovery and Sequence Recovery Using DNA Microarrays |
title_short | Viral Discovery and Sequence Recovery Using DNA Microarrays |
title_sort | viral discovery and sequence recovery using dna microarrays |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC261870/ https://www.ncbi.nlm.nih.gov/pubmed/14624234 http://dx.doi.org/10.1371/journal.pbio.0000002 |
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