Alterations on peripheral B cell subsets following an acute uncomplicated clinical malaria infection in children

BACKGROUND: The effects of Plasmodium falciparum on B-cell homeostasis have not been well characterized. This study investigated whether an episode of acute malaria in young children results in changes in the peripheral B cell phenotype. METHODS: Using flow-cytofluorimetric analysis, the B cell phenotypes found in the peripheral blood of children aged 2–5 years were characterized during an episode of acute uncomplicated clinical malaria and four weeks post-recovery and in healthy age-matched controls. RESULTS: There was a significant decrease in CD19(+ )B lymphocytes during acute malaria. Characterization of the CD19(+ )B cell subsets in the peripheral blood based on expression of IgD and CD38 revealed a significant decrease in the numbers of naive 1 CD38(-)IgD(+ )B cells while there was an increase in CD38(+)IgD(- )memory 3 B cells during acute malaria. Further analysis of the peripheral B cell phenotype also identified an expansion of transitional CD10(+)CD19(+ )B cells in children following an episode of acute malaria with up to 25% of total CD19(+ )B cell pool residing in this subset. CONCLUSION: Children experiencing an episode of acute uncomplicated clinical malaria experienced profound disturbances in B cell homeostasis.