Characterization of the Cdc6 Homologues from the Euryarchaeon Thermoplasma acidophilum

Archaeal cell division cycle protein 6 (Cdc6) homologues are thought to be involved in the initiation process of DNA replication. In the present study, a biochemical characterization of the two Cdc6 proteins from the archaeon Thermoplasma acidophilum has been performed. Both TaCdc6-1 and TaCdc6-2 be...

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Detalles Bibliográficos
Autores principales: Haugland, Gyri Teien, Innselset, Marte, Madern, Dominique, Birkeland, Nils-Kåre
Formato: Texto
Lenguaje:English
Publicado: Bentham Open 2008
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2627522/
https://www.ncbi.nlm.nih.gov/pubmed/19238189
http://dx.doi.org/10.2174/1874091X00802010129
Descripción
Sumario:Archaeal cell division cycle protein 6 (Cdc6) homologues are thought to be involved in the initiation process of DNA replication. In the present study, a biochemical characterization of the two Cdc6 proteins from the archaeon Thermoplasma acidophilum has been performed. Both TaCdc6-1 and TaCdc6-2 behave as monomers in solution and both are abundantly expressed in vivo. Further, TaCdc6-1 shows strong ability to undergo autophosphorylation compared to TaCdc6-2 and the autophosphorylation activity is not affected by DNA or MCM.

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