Characterization of the Cdc6 Homologues from the Euryarchaeon Thermoplasma acidophilum

Archaeal cell division cycle protein 6 (Cdc6) homologues are thought to be involved in the initiation process of DNA replication. In the present study, a biochemical characterization of the two Cdc6 proteins from the archaeon Thermoplasma acidophilum has been performed. Both TaCdc6-1 and TaCdc6-2 be...

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Autores principales: Haugland, Gyri Teien, Innselset, Marte, Madern, Dominique, Birkeland, Nils-Kåre
Formato: Texto
Lenguaje:English
Publicado: Bentham Open 2008
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2627522/
https://www.ncbi.nlm.nih.gov/pubmed/19238189
http://dx.doi.org/10.2174/1874091X00802010129
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author Haugland, Gyri Teien
Innselset, Marte
Madern, Dominique
Birkeland, Nils-Kåre
author_facet Haugland, Gyri Teien
Innselset, Marte
Madern, Dominique
Birkeland, Nils-Kåre
author_sort Haugland, Gyri Teien
collection PubMed
description Archaeal cell division cycle protein 6 (Cdc6) homologues are thought to be involved in the initiation process of DNA replication. In the present study, a biochemical characterization of the two Cdc6 proteins from the archaeon Thermoplasma acidophilum has been performed. Both TaCdc6-1 and TaCdc6-2 behave as monomers in solution and both are abundantly expressed in vivo. Further, TaCdc6-1 shows strong ability to undergo autophosphorylation compared to TaCdc6-2 and the autophosphorylation activity is not affected by DNA or MCM.
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spelling pubmed-26275222009-02-23 Characterization of the Cdc6 Homologues from the Euryarchaeon Thermoplasma acidophilum Haugland, Gyri Teien Innselset, Marte Madern, Dominique Birkeland, Nils-Kåre Open Biochem J Article Archaeal cell division cycle protein 6 (Cdc6) homologues are thought to be involved in the initiation process of DNA replication. In the present study, a biochemical characterization of the two Cdc6 proteins from the archaeon Thermoplasma acidophilum has been performed. Both TaCdc6-1 and TaCdc6-2 behave as monomers in solution and both are abundantly expressed in vivo. Further, TaCdc6-1 shows strong ability to undergo autophosphorylation compared to TaCdc6-2 and the autophosphorylation activity is not affected by DNA or MCM. Bentham Open 2008-09-27 /pmc/articles/PMC2627522/ /pubmed/19238189 http://dx.doi.org/10.2174/1874091X00802010129 Text en © Haugland et al.; Licensee Bentham Open. http://creativecommons.org/licenses/by-nc/3.0/ This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, use, distribution, and reproduction in any medium, provided the work is properly cited.
spellingShingle Article
Haugland, Gyri Teien
Innselset, Marte
Madern, Dominique
Birkeland, Nils-Kåre
Characterization of the Cdc6 Homologues from the Euryarchaeon Thermoplasma acidophilum
title Characterization of the Cdc6 Homologues from the Euryarchaeon Thermoplasma acidophilum
title_full Characterization of the Cdc6 Homologues from the Euryarchaeon Thermoplasma acidophilum
title_fullStr Characterization of the Cdc6 Homologues from the Euryarchaeon Thermoplasma acidophilum
title_full_unstemmed Characterization of the Cdc6 Homologues from the Euryarchaeon Thermoplasma acidophilum
title_short Characterization of the Cdc6 Homologues from the Euryarchaeon Thermoplasma acidophilum
title_sort characterization of the cdc6 homologues from the euryarchaeon thermoplasma acidophilum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2627522/
https://www.ncbi.nlm.nih.gov/pubmed/19238189
http://dx.doi.org/10.2174/1874091X00802010129
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