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Recombination analysis of Soybean mosaic virus sequences reveals evidence of RNA recombination between distinct pathotypes

RNA recombination is one of the two major factors that create RNA genome variability. Assessing its incidence in plant RNA viruses helps understand the formation of new isolates and evaluate the effectiveness of crop protection strategies. To search for recombination in Soybean mosaic virus (SMV), t...

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Autores principales: Gagarinova, Alla G, Babu, Mohan, Strömvik, Martina V, Wang, Aiming
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2627826/
https://www.ncbi.nlm.nih.gov/pubmed/19036160
http://dx.doi.org/10.1186/1743-422X-5-143
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author Gagarinova, Alla G
Babu, Mohan
Strömvik, Martina V
Wang, Aiming
author_facet Gagarinova, Alla G
Babu, Mohan
Strömvik, Martina V
Wang, Aiming
author_sort Gagarinova, Alla G
collection PubMed
description RNA recombination is one of the two major factors that create RNA genome variability. Assessing its incidence in plant RNA viruses helps understand the formation of new isolates and evaluate the effectiveness of crop protection strategies. To search for recombination in Soybean mosaic virus (SMV), the causal agent of a worldwide seed-borne, aphid-transmitted viral soybean disease, we obtained all full-length genome sequences of SMV as well as partial sequences encoding the N-terminal most (P1 protease) and the C-terminal most (capsid protein; CP) viral protein. The sequences were analyzed for possible recombination events using a variety of automatic and manual recombination detection and verification approaches. Automatic scanning identified 3, 10, and 17 recombination sites in the P1, CP, and full-length sequences, respectively. Manual analyses confirmed 10 recombination sites in three full-length SMV sequences. To our knowledge, this is the first report of recombination between distinct SMV pathotypes. These data imply that different SMV pathotypes can simultaneously infect a host cell and exchange genetic materials through recombination. The high incidence of SMV recombination suggests that recombination plays an important role in SMV evolution. Obtaining additional full-length sequences will help elucidate this role.
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spelling pubmed-26278262009-01-17 Recombination analysis of Soybean mosaic virus sequences reveals evidence of RNA recombination between distinct pathotypes Gagarinova, Alla G Babu, Mohan Strömvik, Martina V Wang, Aiming Virol J Short Report RNA recombination is one of the two major factors that create RNA genome variability. Assessing its incidence in plant RNA viruses helps understand the formation of new isolates and evaluate the effectiveness of crop protection strategies. To search for recombination in Soybean mosaic virus (SMV), the causal agent of a worldwide seed-borne, aphid-transmitted viral soybean disease, we obtained all full-length genome sequences of SMV as well as partial sequences encoding the N-terminal most (P1 protease) and the C-terminal most (capsid protein; CP) viral protein. The sequences were analyzed for possible recombination events using a variety of automatic and manual recombination detection and verification approaches. Automatic scanning identified 3, 10, and 17 recombination sites in the P1, CP, and full-length sequences, respectively. Manual analyses confirmed 10 recombination sites in three full-length SMV sequences. To our knowledge, this is the first report of recombination between distinct SMV pathotypes. These data imply that different SMV pathotypes can simultaneously infect a host cell and exchange genetic materials through recombination. The high incidence of SMV recombination suggests that recombination plays an important role in SMV evolution. Obtaining additional full-length sequences will help elucidate this role. BioMed Central 2008-11-26 /pmc/articles/PMC2627826/ /pubmed/19036160 http://dx.doi.org/10.1186/1743-422X-5-143 Text en Copyright © 2008 Gagarinova et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Report
Gagarinova, Alla G
Babu, Mohan
Strömvik, Martina V
Wang, Aiming
Recombination analysis of Soybean mosaic virus sequences reveals evidence of RNA recombination between distinct pathotypes
title Recombination analysis of Soybean mosaic virus sequences reveals evidence of RNA recombination between distinct pathotypes
title_full Recombination analysis of Soybean mosaic virus sequences reveals evidence of RNA recombination between distinct pathotypes
title_fullStr Recombination analysis of Soybean mosaic virus sequences reveals evidence of RNA recombination between distinct pathotypes
title_full_unstemmed Recombination analysis of Soybean mosaic virus sequences reveals evidence of RNA recombination between distinct pathotypes
title_short Recombination analysis of Soybean mosaic virus sequences reveals evidence of RNA recombination between distinct pathotypes
title_sort recombination analysis of soybean mosaic virus sequences reveals evidence of rna recombination between distinct pathotypes
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2627826/
https://www.ncbi.nlm.nih.gov/pubmed/19036160
http://dx.doi.org/10.1186/1743-422X-5-143
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