Prevalence of non-O157:H7 shiga toxin-producing Escherichia coli in diarrheal stool samples from Nebraska.

We determined the prevalence of Shiga toxin-producing Escherichia coli (STEC) in diarrheal stool samples from Nebraska by three methods: cefixime-tellurite sorbitol MacConkey (CT- SMAC) culture, enterohemorrhagic E. coli (EHEC) enzyme immunoassay, and stx1,2 polymerase chain reaction (PCR). Fourteen...

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Autores principales: Fey, P D, Wickert, R S, Rupp, M E, Safranek, T J, Hinrichs, S H
Formato: Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2000
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2627960/
https://www.ncbi.nlm.nih.gov/pubmed/10998385
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author Fey, P D
Wickert, R S
Rupp, M E
Safranek, T J
Hinrichs, S H
author_facet Fey, P D
Wickert, R S
Rupp, M E
Safranek, T J
Hinrichs, S H
author_sort Fey, P D
collection PubMed
description We determined the prevalence of Shiga toxin-producing Escherichia coli (STEC) in diarrheal stool samples from Nebraska by three methods: cefixime-tellurite sorbitol MacConkey (CT- SMAC) culture, enterohemorrhagic E. coli (EHEC) enzyme immunoassay, and stx1,2 polymerase chain reaction (PCR). Fourteen (4.2%) of 335 specimens were positive by at least one method (CT-SMAC culture [6 of 14], EHEC enzyme immunoassay [13 of 14], stx1,2 PCR [14 of 14]). Six contained serogroup O157, while non-O157 were as prevalent as O157 serogroups.
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spelling pubmed-26279602009-05-20 Prevalence of non-O157:H7 shiga toxin-producing Escherichia coli in diarrheal stool samples from Nebraska. Fey, P D Wickert, R S Rupp, M E Safranek, T J Hinrichs, S H Emerg Infect Dis Research Article We determined the prevalence of Shiga toxin-producing Escherichia coli (STEC) in diarrheal stool samples from Nebraska by three methods: cefixime-tellurite sorbitol MacConkey (CT- SMAC) culture, enterohemorrhagic E. coli (EHEC) enzyme immunoassay, and stx1,2 polymerase chain reaction (PCR). Fourteen (4.2%) of 335 specimens were positive by at least one method (CT-SMAC culture [6 of 14], EHEC enzyme immunoassay [13 of 14], stx1,2 PCR [14 of 14]). Six contained serogroup O157, while non-O157 were as prevalent as O157 serogroups. Centers for Disease Control and Prevention 2000 /pmc/articles/PMC2627960/ /pubmed/10998385 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Research Article
Fey, P D
Wickert, R S
Rupp, M E
Safranek, T J
Hinrichs, S H
Prevalence of non-O157:H7 shiga toxin-producing Escherichia coli in diarrheal stool samples from Nebraska.
title Prevalence of non-O157:H7 shiga toxin-producing Escherichia coli in diarrheal stool samples from Nebraska.
title_full Prevalence of non-O157:H7 shiga toxin-producing Escherichia coli in diarrheal stool samples from Nebraska.
title_fullStr Prevalence of non-O157:H7 shiga toxin-producing Escherichia coli in diarrheal stool samples from Nebraska.
title_full_unstemmed Prevalence of non-O157:H7 shiga toxin-producing Escherichia coli in diarrheal stool samples from Nebraska.
title_short Prevalence of non-O157:H7 shiga toxin-producing Escherichia coli in diarrheal stool samples from Nebraska.
title_sort prevalence of non-o157:h7 shiga toxin-producing escherichia coli in diarrheal stool samples from nebraska.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2627960/
https://www.ncbi.nlm.nih.gov/pubmed/10998385
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