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Molecular profiling of T-helper immune genes during dengue virus infection
In this study, we provide a comprehensive molecular profiling of the involvement of T- helper (Th) genes during dengue virus infection of different cell types. The Th gene profiles of three human cell types (monocytes, T-cells and hepatocytes) were analyzed simultaneously via array-based RT-PCR upon...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2008
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2628356/ https://www.ncbi.nlm.nih.gov/pubmed/19117515 http://dx.doi.org/10.1186/1743-422X-5-165 |
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author | Chen, Jincheng Ng, Mary Mah Lee Chu, Justin Jang Hann |
author_facet | Chen, Jincheng Ng, Mary Mah Lee Chu, Justin Jang Hann |
author_sort | Chen, Jincheng |
collection | PubMed |
description | In this study, we provide a comprehensive molecular profiling of the involvement of T- helper (Th) genes during dengue virus infection of different cell types. The Th gene profiles of three human cell types (monocytes, T-cells and hepatocytes) were analyzed simultaneously via array-based RT-PCR upon infection with dengue virus. Differential regulation of 41 Th genes was identified and of which 20 of those genes may contribute to immuno-pathogenesis of dengue virus infection by regulating inflammation, thrombocytopenia and vascular permeability. Among the strongly up-regulated genes were the RANTES, CC-CKR3, IRF4, CLEC2C, IL-6 and TLR6, which are potent inducer of inflammation and vascular permeability. Profiling genes obtained from this study may serve as potential biomarkers and the modulation of Th immune responses during dengue virus infection has important implications in disease outcome. |
format | Text |
id | pubmed-2628356 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-26283562009-01-17 Molecular profiling of T-helper immune genes during dengue virus infection Chen, Jincheng Ng, Mary Mah Lee Chu, Justin Jang Hann Virol J Short Report In this study, we provide a comprehensive molecular profiling of the involvement of T- helper (Th) genes during dengue virus infection of different cell types. The Th gene profiles of three human cell types (monocytes, T-cells and hepatocytes) were analyzed simultaneously via array-based RT-PCR upon infection with dengue virus. Differential regulation of 41 Th genes was identified and of which 20 of those genes may contribute to immuno-pathogenesis of dengue virus infection by regulating inflammation, thrombocytopenia and vascular permeability. Among the strongly up-regulated genes were the RANTES, CC-CKR3, IRF4, CLEC2C, IL-6 and TLR6, which are potent inducer of inflammation and vascular permeability. Profiling genes obtained from this study may serve as potential biomarkers and the modulation of Th immune responses during dengue virus infection has important implications in disease outcome. BioMed Central 2008-12-31 /pmc/articles/PMC2628356/ /pubmed/19117515 http://dx.doi.org/10.1186/1743-422X-5-165 Text en Copyright © 2008 Chen et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Report Chen, Jincheng Ng, Mary Mah Lee Chu, Justin Jang Hann Molecular profiling of T-helper immune genes during dengue virus infection |
title | Molecular profiling of T-helper immune genes during dengue virus infection |
title_full | Molecular profiling of T-helper immune genes during dengue virus infection |
title_fullStr | Molecular profiling of T-helper immune genes during dengue virus infection |
title_full_unstemmed | Molecular profiling of T-helper immune genes during dengue virus infection |
title_short | Molecular profiling of T-helper immune genes during dengue virus infection |
title_sort | molecular profiling of t-helper immune genes during dengue virus infection |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2628356/ https://www.ncbi.nlm.nih.gov/pubmed/19117515 http://dx.doi.org/10.1186/1743-422X-5-165 |
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