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ruvA and ruvB mutants specifically impaired for replication fork reversal
Replication fork reversal (RFR) is a reaction that takes place in Escherichia coli at replication forks arrested by the inactivation of a replication protein. Fork reversal involves the annealing of the leading and lagging strand ends; it results in the formation of a Holliday junction adjacent to D...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2628435/ https://www.ncbi.nlm.nih.gov/pubmed/18942176 http://dx.doi.org/10.1111/j.1365-2958.2008.06431.x |
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author | Le Masson, Marie Baharoglu, Zeynep Michel, Bénédicte |
author_facet | Le Masson, Marie Baharoglu, Zeynep Michel, Bénédicte |
author_sort | Le Masson, Marie |
collection | PubMed |
description | Replication fork reversal (RFR) is a reaction that takes place in Escherichia coli at replication forks arrested by the inactivation of a replication protein. Fork reversal involves the annealing of the leading and lagging strand ends; it results in the formation of a Holliday junction adjacent to DNA double-strand end, both of which are processed by recombination enzymes. In several replication mutants, replication fork reversal is catalysed by the RuvAB complex, originally characterized for its role in the last steps of homologous recombination, branch migration and resolution of Holliday junctions. We present here the isolation and characterization of ruvA and ruvB single mutants that are impaired for RFR at forks arrested by the inactivation of polymerase III, while they remain capable of homologous recombination. The positions of the mutations in the proteins and the genetic properties of the mutants suggest that the mutations affect DNA binding, RuvA–RuvB interaction and/or RuvB-helicase activity. These results show that a partial RuvA or RuvB defect affects primarily RFR, implying that RFR is a more demanding reaction than Holliday junction resolution. |
format | Text |
id | pubmed-2628435 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-26284352009-01-27 ruvA and ruvB mutants specifically impaired for replication fork reversal Le Masson, Marie Baharoglu, Zeynep Michel, Bénédicte Mol Microbiol Research Articles Replication fork reversal (RFR) is a reaction that takes place in Escherichia coli at replication forks arrested by the inactivation of a replication protein. Fork reversal involves the annealing of the leading and lagging strand ends; it results in the formation of a Holliday junction adjacent to DNA double-strand end, both of which are processed by recombination enzymes. In several replication mutants, replication fork reversal is catalysed by the RuvAB complex, originally characterized for its role in the last steps of homologous recombination, branch migration and resolution of Holliday junctions. We present here the isolation and characterization of ruvA and ruvB single mutants that are impaired for RFR at forks arrested by the inactivation of polymerase III, while they remain capable of homologous recombination. The positions of the mutations in the proteins and the genetic properties of the mutants suggest that the mutations affect DNA binding, RuvA–RuvB interaction and/or RuvB-helicase activity. These results show that a partial RuvA or RuvB defect affects primarily RFR, implying that RFR is a more demanding reaction than Holliday junction resolution. Blackwell Publishing Ltd 2008-10 2008-09-15 /pmc/articles/PMC2628435/ /pubmed/18942176 http://dx.doi.org/10.1111/j.1365-2958.2008.06431.x Text en © 2008 The Authors Journal compilation © 2008 Blackwell Publishing Ltd |
spellingShingle | Research Articles Le Masson, Marie Baharoglu, Zeynep Michel, Bénédicte ruvA and ruvB mutants specifically impaired for replication fork reversal |
title | ruvA and ruvB mutants specifically impaired for replication fork reversal |
title_full | ruvA and ruvB mutants specifically impaired for replication fork reversal |
title_fullStr | ruvA and ruvB mutants specifically impaired for replication fork reversal |
title_full_unstemmed | ruvA and ruvB mutants specifically impaired for replication fork reversal |
title_short | ruvA and ruvB mutants specifically impaired for replication fork reversal |
title_sort | ruva and ruvb mutants specifically impaired for replication fork reversal |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2628435/ https://www.ncbi.nlm.nih.gov/pubmed/18942176 http://dx.doi.org/10.1111/j.1365-2958.2008.06431.x |
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