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An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol
BACKGROUND: Mutagenesis plays an essential role in molecular biology and biochemistry. It has also been used in enzymology and protein science to generate proteins which are more tractable for biophysical techniques. The ability to quickly and specifically mutate a residue(s) in protein is important...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2008
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2629768/ https://www.ncbi.nlm.nih.gov/pubmed/19055817 http://dx.doi.org/10.1186/1472-6750-8-91 |