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High correlation of the proteome patterns in bone marrow and peripheral blood blast cells in patients with acute myeloid leukemia

BACKGROUND: When comparing myelogenous blasts from bone marrow and peripheral blood, immunophenotyping usually show a strong correlation of expression of surface antigens. However, it remains to be determined, whether this correlation also exists on the level of protein expression. METHOD: Therefore...

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Autores principales: Hütter, Gero, Letsch, Anne, Nowak, Daniel, Poland, Julia, Sinha, Pranav, Thiel, Eckhard, Hofmann, Wolf-K
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2630935/
https://www.ncbi.nlm.nih.gov/pubmed/19146667
http://dx.doi.org/10.1186/1479-5876-7-7
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author Hütter, Gero
Letsch, Anne
Nowak, Daniel
Poland, Julia
Sinha, Pranav
Thiel, Eckhard
Hofmann, Wolf-K
author_facet Hütter, Gero
Letsch, Anne
Nowak, Daniel
Poland, Julia
Sinha, Pranav
Thiel, Eckhard
Hofmann, Wolf-K
author_sort Hütter, Gero
collection PubMed
description BACKGROUND: When comparing myelogenous blasts from bone marrow and peripheral blood, immunophenotyping usually show a strong correlation of expression of surface antigens. However, it remains to be determined, whether this correlation also exists on the level of protein expression. METHOD: Therefore, we investigated both bone marrow and peripheral blood blast cells from six patients with newly diagnosed acute myeloid leukemia (AML) using conventional two-dimensional electrophoresis in the first dimension and linear polyacrylamide gels (12%) in the second dimension. Proteins were visualized using the silver staining method and image analysis was performed using the PDQuest system. RESULTS: For each patient over 80 proteins were evaluated in the sample from peripheral blood and bone marrow. We could demonstrate that the protein expression profile of bone marrow did not significantly differ from the expression patterns of peripheral blast cells. CONCLUSION: The proteome-set of leukemic blast cells from marrow and blood, does not differ substantially when drawn from AML patients with over 80 percent blast cells in both compartments. This indicates that in AML, blasts from peripheral blood samples can be considered suitable for investigations of the proteome using 2D-electrophoresis.
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spelling pubmed-26309352009-01-27 High correlation of the proteome patterns in bone marrow and peripheral blood blast cells in patients with acute myeloid leukemia Hütter, Gero Letsch, Anne Nowak, Daniel Poland, Julia Sinha, Pranav Thiel, Eckhard Hofmann, Wolf-K J Transl Med Methodology BACKGROUND: When comparing myelogenous blasts from bone marrow and peripheral blood, immunophenotyping usually show a strong correlation of expression of surface antigens. However, it remains to be determined, whether this correlation also exists on the level of protein expression. METHOD: Therefore, we investigated both bone marrow and peripheral blood blast cells from six patients with newly diagnosed acute myeloid leukemia (AML) using conventional two-dimensional electrophoresis in the first dimension and linear polyacrylamide gels (12%) in the second dimension. Proteins were visualized using the silver staining method and image analysis was performed using the PDQuest system. RESULTS: For each patient over 80 proteins were evaluated in the sample from peripheral blood and bone marrow. We could demonstrate that the protein expression profile of bone marrow did not significantly differ from the expression patterns of peripheral blast cells. CONCLUSION: The proteome-set of leukemic blast cells from marrow and blood, does not differ substantially when drawn from AML patients with over 80 percent blast cells in both compartments. This indicates that in AML, blasts from peripheral blood samples can be considered suitable for investigations of the proteome using 2D-electrophoresis. BioMed Central 2009-01-15 /pmc/articles/PMC2630935/ /pubmed/19146667 http://dx.doi.org/10.1186/1479-5876-7-7 Text en Copyright © 2009 Hütter et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology
Hütter, Gero
Letsch, Anne
Nowak, Daniel
Poland, Julia
Sinha, Pranav
Thiel, Eckhard
Hofmann, Wolf-K
High correlation of the proteome patterns in bone marrow and peripheral blood blast cells in patients with acute myeloid leukemia
title High correlation of the proteome patterns in bone marrow and peripheral blood blast cells in patients with acute myeloid leukemia
title_full High correlation of the proteome patterns in bone marrow and peripheral blood blast cells in patients with acute myeloid leukemia
title_fullStr High correlation of the proteome patterns in bone marrow and peripheral blood blast cells in patients with acute myeloid leukemia
title_full_unstemmed High correlation of the proteome patterns in bone marrow and peripheral blood blast cells in patients with acute myeloid leukemia
title_short High correlation of the proteome patterns in bone marrow and peripheral blood blast cells in patients with acute myeloid leukemia
title_sort high correlation of the proteome patterns in bone marrow and peripheral blood blast cells in patients with acute myeloid leukemia
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2630935/
https://www.ncbi.nlm.nih.gov/pubmed/19146667
http://dx.doi.org/10.1186/1479-5876-7-7
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