Nested polymerase chain reaction for amplification of the Cryptosporidium oocyst wall protein gene.

We developed a sensitive nested polymerase chain reaction procedure for the Cryptosporidium oocyst wall protein (COWP) gene. Amplification and genotyping were successful in 95.2% of 1,680 fecal samples, 77.6% by the unnested and 17.6% by the nested COWP procedure. The COWP gene was amplified from 2,...

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Detalles Bibliográficos
Autores principales: Pedraza-Díaz, S, Amar, C, Nichols, G L, McLauchlin, J
Formato: Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2001
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2631669/
https://www.ncbi.nlm.nih.gov/pubmed/11266294
Descripción
Sumario:We developed a sensitive nested polymerase chain reaction procedure for the Cryptosporidium oocyst wall protein (COWP) gene. Amplification and genotyping were successful in 95.2% of 1,680 fecal samples, 77.6% by the unnested and 17.6% by the nested COWP procedure. The COWP gene was amplified from 2,128 fecal samples: 71 from livestock animals and 2,057 from humans. This series included 706 cases from seven drinking water-associated outbreaks and 51 cases from five swimming pool-associated outbreaks, as well as 1,300 sporadic cases.

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