Nested polymerase chain reaction for amplification of the Cryptosporidium oocyst wall protein gene.

We developed a sensitive nested polymerase chain reaction procedure for the Cryptosporidium oocyst wall protein (COWP) gene. Amplification and genotyping were successful in 95.2% of 1,680 fecal samples, 77.6% by the unnested and 17.6% by the nested COWP procedure. The COWP gene was amplified from 2,...

Descripción completa

Detalles Bibliográficos
Autores principales: Pedraza-Díaz, S, Amar, C, Nichols, G L, McLauchlin, J
Formato: Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2001
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2631669/
https://www.ncbi.nlm.nih.gov/pubmed/11266294
_version_ 1782163957566406656
author Pedraza-Díaz, S
Amar, C
Nichols, G L
McLauchlin, J
author_facet Pedraza-Díaz, S
Amar, C
Nichols, G L
McLauchlin, J
author_sort Pedraza-Díaz, S
collection PubMed
description We developed a sensitive nested polymerase chain reaction procedure for the Cryptosporidium oocyst wall protein (COWP) gene. Amplification and genotyping were successful in 95.2% of 1,680 fecal samples, 77.6% by the unnested and 17.6% by the nested COWP procedure. The COWP gene was amplified from 2,128 fecal samples: 71 from livestock animals and 2,057 from humans. This series included 706 cases from seven drinking water-associated outbreaks and 51 cases from five swimming pool-associated outbreaks, as well as 1,300 sporadic cases.
format Text
id pubmed-2631669
institution National Center for Biotechnology Information
language English
publishDate 2001
publisher Centers for Disease Control and Prevention
record_format MEDLINE/PubMed
spelling pubmed-26316692009-05-20 Nested polymerase chain reaction for amplification of the Cryptosporidium oocyst wall protein gene. Pedraza-Díaz, S Amar, C Nichols, G L McLauchlin, J Emerg Infect Dis Research Article We developed a sensitive nested polymerase chain reaction procedure for the Cryptosporidium oocyst wall protein (COWP) gene. Amplification and genotyping were successful in 95.2% of 1,680 fecal samples, 77.6% by the unnested and 17.6% by the nested COWP procedure. The COWP gene was amplified from 2,128 fecal samples: 71 from livestock animals and 2,057 from humans. This series included 706 cases from seven drinking water-associated outbreaks and 51 cases from five swimming pool-associated outbreaks, as well as 1,300 sporadic cases. Centers for Disease Control and Prevention 2001 /pmc/articles/PMC2631669/ /pubmed/11266294 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Research Article
Pedraza-Díaz, S
Amar, C
Nichols, G L
McLauchlin, J
Nested polymerase chain reaction for amplification of the Cryptosporidium oocyst wall protein gene.
title Nested polymerase chain reaction for amplification of the Cryptosporidium oocyst wall protein gene.
title_full Nested polymerase chain reaction for amplification of the Cryptosporidium oocyst wall protein gene.
title_fullStr Nested polymerase chain reaction for amplification of the Cryptosporidium oocyst wall protein gene.
title_full_unstemmed Nested polymerase chain reaction for amplification of the Cryptosporidium oocyst wall protein gene.
title_short Nested polymerase chain reaction for amplification of the Cryptosporidium oocyst wall protein gene.
title_sort nested polymerase chain reaction for amplification of the cryptosporidium oocyst wall protein gene.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2631669/
https://www.ncbi.nlm.nih.gov/pubmed/11266294
work_keys_str_mv AT pedrazadiazs nestedpolymerasechainreactionforamplificationofthecryptosporidiumoocystwallproteingene
AT amarc nestedpolymerasechainreactionforamplificationofthecryptosporidiumoocystwallproteingene
AT nicholsgl nestedpolymerasechainreactionforamplificationofthecryptosporidiumoocystwallproteingene
AT mclauchlinj nestedpolymerasechainreactionforamplificationofthecryptosporidiumoocystwallproteingene

Ejemplares similares