Partial genetic characterization of West Nile virus strains, New York State, 2000.

We analyzed nucleotide sequences from the envelope gene of 11 West Nile (WN) virus strains collected in New York State during the 2000 transmission season to determine whether they differed genetically from each other and from the initial strain isolated in 1999. The complete envelope genes of these...

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Autores principales: Ebel, G D, Dupuis, A P, Ngo, K, Nicholas, D, Kauffman, E, Jones, S A, Young, D, Maffei, J, Shi, P Y, Bernard, K, Kramer, L D
Formato: Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2001
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2631742/
https://www.ncbi.nlm.nih.gov/pubmed/11585527
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author Ebel, G D
Dupuis, A P
Ngo, K
Nicholas, D
Kauffman, E
Jones, S A
Young, D
Maffei, J
Shi, P Y
Bernard, K
Kramer, L D
author_facet Ebel, G D
Dupuis, A P
Ngo, K
Nicholas, D
Kauffman, E
Jones, S A
Young, D
Maffei, J
Shi, P Y
Bernard, K
Kramer, L D
author_sort Ebel, G D
collection PubMed
description We analyzed nucleotide sequences from the envelope gene of 11 West Nile (WN) virus strains collected in New York State during the 2000 transmission season to determine whether they differed genetically from each other and from the initial strain isolated in 1999. The complete envelope genes of these strains were amplified by reverse transcription-polymerase chain reaction. The resulting sequences were aligned, the genetic distances were computed, and a phylogenetic tree was constructed. Ten (0.7%) of 1,503 positions in the envelope gene were polymorphic in one or more sequences. The genetic distances were 0.003 or less. WN virus strains circulating in 2000 were homogeneous with respect to one another and to a strain isolated in 1999.
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spelling pubmed-26317422009-05-20 Partial genetic characterization of West Nile virus strains, New York State, 2000. Ebel, G D Dupuis, A P Ngo, K Nicholas, D Kauffman, E Jones, S A Young, D Maffei, J Shi, P Y Bernard, K Kramer, L D Emerg Infect Dis Research Article We analyzed nucleotide sequences from the envelope gene of 11 West Nile (WN) virus strains collected in New York State during the 2000 transmission season to determine whether they differed genetically from each other and from the initial strain isolated in 1999. The complete envelope genes of these strains were amplified by reverse transcription-polymerase chain reaction. The resulting sequences were aligned, the genetic distances were computed, and a phylogenetic tree was constructed. Ten (0.7%) of 1,503 positions in the envelope gene were polymorphic in one or more sequences. The genetic distances were 0.003 or less. WN virus strains circulating in 2000 were homogeneous with respect to one another and to a strain isolated in 1999. Centers for Disease Control and Prevention 2001 /pmc/articles/PMC2631742/ /pubmed/11585527 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Research Article
Ebel, G D
Dupuis, A P
Ngo, K
Nicholas, D
Kauffman, E
Jones, S A
Young, D
Maffei, J
Shi, P Y
Bernard, K
Kramer, L D
Partial genetic characterization of West Nile virus strains, New York State, 2000.
title Partial genetic characterization of West Nile virus strains, New York State, 2000.
title_full Partial genetic characterization of West Nile virus strains, New York State, 2000.
title_fullStr Partial genetic characterization of West Nile virus strains, New York State, 2000.
title_full_unstemmed Partial genetic characterization of West Nile virus strains, New York State, 2000.
title_short Partial genetic characterization of West Nile virus strains, New York State, 2000.
title_sort partial genetic characterization of west nile virus strains, new york state, 2000.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2631742/
https://www.ncbi.nlm.nih.gov/pubmed/11585527
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