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Rapid determination of HLA B*07 ligands from the West Nile virus NY99 genome.

Defined T cell epitopes for West Nile (WN) virus may be useful for developing subunit vaccines against WN virus infection and diagnostic reagents to detect WN virus-specific immune response. We applied a bioinformatics (EpiMatrix) approach to search the WN virus NY99 genome for HLA B*07 restricted c...

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Detalles Bibliográficos
Autores principales: De Groot, A S, Saint-Aubin, C, Bosma, A, Sbai, H, Rayner, J, Martin, W
Formato: Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2001
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2631750/
https://www.ncbi.nlm.nih.gov/pubmed/11585536
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author De Groot, A S
Saint-Aubin, C
Bosma, A
Sbai, H
Rayner, J
Martin, W
author_facet De Groot, A S
Saint-Aubin, C
Bosma, A
Sbai, H
Rayner, J
Martin, W
author_sort De Groot, A S
collection PubMed
description Defined T cell epitopes for West Nile (WN) virus may be useful for developing subunit vaccines against WN virus infection and diagnostic reagents to detect WN virus-specific immune response. We applied a bioinformatics (EpiMatrix) approach to search the WN virus NY99 genome for HLA B*07 restricted cytotoxic T cell (CTL) epitopes. Ninety-five of 3,433 WN virus peptides scored above a predetermined cutoff, suggesting that these would be likely to bind to HLA B*07 and would also be likely candidate CTL epitopes. Compared with other methods for genome mapping, derivation of these ligands was rapid and inexpensive. Major histocompatibility complex ligands identified by this method may be used to screen T cells from WN virus-exposed persons for cell-mediated response to WN virus or to develop diagnostic reagents for immunopathogenesis studies and epidemiologic surveillance.
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spelling pubmed-26317502009-05-20 Rapid determination of HLA B*07 ligands from the West Nile virus NY99 genome. De Groot, A S Saint-Aubin, C Bosma, A Sbai, H Rayner, J Martin, W Emerg Infect Dis Research Article Defined T cell epitopes for West Nile (WN) virus may be useful for developing subunit vaccines against WN virus infection and diagnostic reagents to detect WN virus-specific immune response. We applied a bioinformatics (EpiMatrix) approach to search the WN virus NY99 genome for HLA B*07 restricted cytotoxic T cell (CTL) epitopes. Ninety-five of 3,433 WN virus peptides scored above a predetermined cutoff, suggesting that these would be likely to bind to HLA B*07 and would also be likely candidate CTL epitopes. Compared with other methods for genome mapping, derivation of these ligands was rapid and inexpensive. Major histocompatibility complex ligands identified by this method may be used to screen T cells from WN virus-exposed persons for cell-mediated response to WN virus or to develop diagnostic reagents for immunopathogenesis studies and epidemiologic surveillance. Centers for Disease Control and Prevention 2001 /pmc/articles/PMC2631750/ /pubmed/11585536 Text en
spellingShingle Research Article
De Groot, A S
Saint-Aubin, C
Bosma, A
Sbai, H
Rayner, J
Martin, W
Rapid determination of HLA B*07 ligands from the West Nile virus NY99 genome.
title Rapid determination of HLA B*07 ligands from the West Nile virus NY99 genome.
title_full Rapid determination of HLA B*07 ligands from the West Nile virus NY99 genome.
title_fullStr Rapid determination of HLA B*07 ligands from the West Nile virus NY99 genome.
title_full_unstemmed Rapid determination of HLA B*07 ligands from the West Nile virus NY99 genome.
title_short Rapid determination of HLA B*07 ligands from the West Nile virus NY99 genome.
title_sort rapid determination of hla b*07 ligands from the west nile virus ny99 genome.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2631750/
https://www.ncbi.nlm.nih.gov/pubmed/11585536
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