Detection of North American West Nile virus in animal tissue by a reverse transcription-nested polymerase chain reaction assay.

A traditional single-stage reverse transcription-polymerase chain reaction (RT-PCR) procedure is effective in determining West Nile (WN) virus in avian tissue and infected cell cultures. However, the procedure lacks the sensitivity to detect WN virus in equine tissue. We describe an RT-nested PCR (R...

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Autores principales: Johnson, D J, Ostlund, E N, Pedersen, D D, Schmitt, B J
Formato: Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2001
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2631755/
https://www.ncbi.nlm.nih.gov/pubmed/11585541
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author Johnson, D J
Ostlund, E N
Pedersen, D D
Schmitt, B J
author_facet Johnson, D J
Ostlund, E N
Pedersen, D D
Schmitt, B J
author_sort Johnson, D J
collection PubMed
description A traditional single-stage reverse transcription-polymerase chain reaction (RT-PCR) procedure is effective in determining West Nile (WN) virus in avian tissue and infected cell cultures. However, the procedure lacks the sensitivity to detect WN virus in equine tissue. We describe an RT-nested PCR (RT-nPCR) procedure that identifies the North American strain of WN virus directly in equine and avian tissues.
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spelling pubmed-26317552009-05-20 Detection of North American West Nile virus in animal tissue by a reverse transcription-nested polymerase chain reaction assay. Johnson, D J Ostlund, E N Pedersen, D D Schmitt, B J Emerg Infect Dis Research Article A traditional single-stage reverse transcription-polymerase chain reaction (RT-PCR) procedure is effective in determining West Nile (WN) virus in avian tissue and infected cell cultures. However, the procedure lacks the sensitivity to detect WN virus in equine tissue. We describe an RT-nested PCR (RT-nPCR) procedure that identifies the North American strain of WN virus directly in equine and avian tissues. Centers for Disease Control and Prevention 2001 /pmc/articles/PMC2631755/ /pubmed/11585541 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Research Article
Johnson, D J
Ostlund, E N
Pedersen, D D
Schmitt, B J
Detection of North American West Nile virus in animal tissue by a reverse transcription-nested polymerase chain reaction assay.
title Detection of North American West Nile virus in animal tissue by a reverse transcription-nested polymerase chain reaction assay.
title_full Detection of North American West Nile virus in animal tissue by a reverse transcription-nested polymerase chain reaction assay.
title_fullStr Detection of North American West Nile virus in animal tissue by a reverse transcription-nested polymerase chain reaction assay.
title_full_unstemmed Detection of North American West Nile virus in animal tissue by a reverse transcription-nested polymerase chain reaction assay.
title_short Detection of North American West Nile virus in animal tissue by a reverse transcription-nested polymerase chain reaction assay.
title_sort detection of north american west nile virus in animal tissue by a reverse transcription-nested polymerase chain reaction assay.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2631755/
https://www.ncbi.nlm.nih.gov/pubmed/11585541
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AT pedersendd detectionofnorthamericanwestnilevirusinanimaltissuebyareversetranscriptionnestedpolymerasechainreactionassay
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