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Transcript profiles uncover temporal and stress-induced changes of metabolic pathways in germinating sugar beet seeds

BACKGROUND: With a cultivation area of 1.75 Mio ha and sugar yield of 16.7 Mio tons in 2006, sugar beet is a crop of great economic importance in Europe. The productivity of sugar beet is determined significantly by seed vigour and field emergence potential; however, little is known about the molecu...

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Autores principales: Pestsova, Elena, Meinhard, Juliane, Menze, Andreas, Fischer, Uwe, Windhövel, Andrea, Westhoff, Peter
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2632670/
https://www.ncbi.nlm.nih.gov/pubmed/19046420
http://dx.doi.org/10.1186/1471-2229-8-122
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author Pestsova, Elena
Meinhard, Juliane
Menze, Andreas
Fischer, Uwe
Windhövel, Andrea
Westhoff, Peter
author_facet Pestsova, Elena
Meinhard, Juliane
Menze, Andreas
Fischer, Uwe
Windhövel, Andrea
Westhoff, Peter
author_sort Pestsova, Elena
collection PubMed
description BACKGROUND: With a cultivation area of 1.75 Mio ha and sugar yield of 16.7 Mio tons in 2006, sugar beet is a crop of great economic importance in Europe. The productivity of sugar beet is determined significantly by seed vigour and field emergence potential; however, little is known about the molecular mechanisms underlying these traits. Both traits exhibit large variations within sugar beet germplasm that have been difficult to ascribe to either environmental or genetic causes. Among potential targets for trait improvement, an enhancement of stress tolerance is considered because of the high negative influence of environmental stresses on trait parameters. Extending our knowledge of genetic and molecular determinants of sugar beet germination, stress response and adaptation mechanisms would facilitate the detection of new targets for breeding crop with an enhanced field emergence potential. RESULTS: To gain insight into the sugar beet germination we initiated an analysis of gene expression in a well emerging sugar beet hybrid showing high germination potential under various environmental conditions. A total of 2,784 ESTs representing 2,251 'unigenes' was generated from dry mature and germinating seeds. Analysis of the temporal expression of these genes during germination under non-stress conditions uncovered drastic transcriptional changes accompanying a shift from quiescent to metabolically active stages of the plant life cycle. Assay of germination under stressful conditions revealed 157 genes showing significantly different expression patterns in response to stress. As deduced from transcriptome data, stress adaptation mechanisms included an alteration in reserve mobilization pathways, an accumulation of the osmoprotectant glycine betaine, late embryogenesis abundant proteins and detoxification enzymes. The observed transcriptional changes are supposed to be regulated by ABA-dependent signal transduction pathway. CONCLUSION: This study provides an important step toward the understanding of main events and metabolic pathways during germination in sugar beet. The reported alterations of gene expression in response to stress shed light on sugar beet stress adaptation mechanisms. Some of the identified stress-responsive genes provide a new potential source for improvement of sugar beet stress tolerance during germination and field emergence.
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spelling pubmed-26326702009-01-29 Transcript profiles uncover temporal and stress-induced changes of metabolic pathways in germinating sugar beet seeds Pestsova, Elena Meinhard, Juliane Menze, Andreas Fischer, Uwe Windhövel, Andrea Westhoff, Peter BMC Plant Biol Research Article BACKGROUND: With a cultivation area of 1.75 Mio ha and sugar yield of 16.7 Mio tons in 2006, sugar beet is a crop of great economic importance in Europe. The productivity of sugar beet is determined significantly by seed vigour and field emergence potential; however, little is known about the molecular mechanisms underlying these traits. Both traits exhibit large variations within sugar beet germplasm that have been difficult to ascribe to either environmental or genetic causes. Among potential targets for trait improvement, an enhancement of stress tolerance is considered because of the high negative influence of environmental stresses on trait parameters. Extending our knowledge of genetic and molecular determinants of sugar beet germination, stress response and adaptation mechanisms would facilitate the detection of new targets for breeding crop with an enhanced field emergence potential. RESULTS: To gain insight into the sugar beet germination we initiated an analysis of gene expression in a well emerging sugar beet hybrid showing high germination potential under various environmental conditions. A total of 2,784 ESTs representing 2,251 'unigenes' was generated from dry mature and germinating seeds. Analysis of the temporal expression of these genes during germination under non-stress conditions uncovered drastic transcriptional changes accompanying a shift from quiescent to metabolically active stages of the plant life cycle. Assay of germination under stressful conditions revealed 157 genes showing significantly different expression patterns in response to stress. As deduced from transcriptome data, stress adaptation mechanisms included an alteration in reserve mobilization pathways, an accumulation of the osmoprotectant glycine betaine, late embryogenesis abundant proteins and detoxification enzymes. The observed transcriptional changes are supposed to be regulated by ABA-dependent signal transduction pathway. CONCLUSION: This study provides an important step toward the understanding of main events and metabolic pathways during germination in sugar beet. The reported alterations of gene expression in response to stress shed light on sugar beet stress adaptation mechanisms. Some of the identified stress-responsive genes provide a new potential source for improvement of sugar beet stress tolerance during germination and field emergence. BioMed Central 2008-12-01 /pmc/articles/PMC2632670/ /pubmed/19046420 http://dx.doi.org/10.1186/1471-2229-8-122 Text en Copyright © 2008 Pestsova et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Pestsova, Elena
Meinhard, Juliane
Menze, Andreas
Fischer, Uwe
Windhövel, Andrea
Westhoff, Peter
Transcript profiles uncover temporal and stress-induced changes of metabolic pathways in germinating sugar beet seeds
title Transcript profiles uncover temporal and stress-induced changes of metabolic pathways in germinating sugar beet seeds
title_full Transcript profiles uncover temporal and stress-induced changes of metabolic pathways in germinating sugar beet seeds
title_fullStr Transcript profiles uncover temporal and stress-induced changes of metabolic pathways in germinating sugar beet seeds
title_full_unstemmed Transcript profiles uncover temporal and stress-induced changes of metabolic pathways in germinating sugar beet seeds
title_short Transcript profiles uncover temporal and stress-induced changes of metabolic pathways in germinating sugar beet seeds
title_sort transcript profiles uncover temporal and stress-induced changes of metabolic pathways in germinating sugar beet seeds
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2632670/
https://www.ncbi.nlm.nih.gov/pubmed/19046420
http://dx.doi.org/10.1186/1471-2229-8-122
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