Cargando…

A Filamentous Hemagglutinin-Like Protein of Xanthomonas axonopodis pv. citri, the Phytopathogen Responsible for Citrus Canker, Is Involved in Bacterial Virulence

Xanthomonas axonopodis pv. citri, the phytopathogen responsible for citrus canker has a number of protein secretion systems and among them, at least one type V protein secretion system belonging to the two-partner secretion pathway. This system is mainly associated to the translocation of large prot...

Descripción completa

Detalles Bibliográficos
Autores principales: Gottig, Natalia, Garavaglia, Betiana S., Garofalo, Cecilia G., Orellano, Elena G., Ottado, Jorgelina
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2632755/
https://www.ncbi.nlm.nih.gov/pubmed/19194503
http://dx.doi.org/10.1371/journal.pone.0004358
_version_ 1782164045358432256
author Gottig, Natalia
Garavaglia, Betiana S.
Garofalo, Cecilia G.
Orellano, Elena G.
Ottado, Jorgelina
author_facet Gottig, Natalia
Garavaglia, Betiana S.
Garofalo, Cecilia G.
Orellano, Elena G.
Ottado, Jorgelina
author_sort Gottig, Natalia
collection PubMed
description Xanthomonas axonopodis pv. citri, the phytopathogen responsible for citrus canker has a number of protein secretion systems and among them, at least one type V protein secretion system belonging to the two-partner secretion pathway. This system is mainly associated to the translocation of large proteins such as adhesins to the outer membrane of several pathogens. Xanthomonas axonopodis pv. citri possess a filamentous hemagglutinin-like protein in close vicinity to its putative transporter protein, XacFhaB and XacFhaC, respectively. Expression analysis indicated that XacFhaB was induced in planta during plant-pathogen interaction. By mutation analysis of XacFhaB and XacFhaC genes we determined that XacFhaB is involved in virulence both in epiphytic and wound inoculations, displaying more dispersed and fewer canker lesions. Unexpectedly, the XacFhaC mutant in the transporter protein produced an intermediate virulence phenotype resembling wild type infection, suggesting that XacFhaB could be secreted by another partner different from XacFhaC. Moreover, XacFhaB mutants showed a general lack of adhesion and were affected in leaf surface attachment and biofilm formation. In agreement with the in planta phenotype, adhesin lacking cells moved faster in swarming plates. Since no hyperflagellation phenotype was observed in this bacteria, the faster movement may be attributed to the lack of cell-to-cell aggregation. Moreover, XacFhaB mutants secreted more exopolysaccharide that in turn may facilitate its motility. Our results suggest that this hemagglutinin-like protein is required for tissue colonization being mainly involved in surface attachment and biofilm formation, and that plant tissue attachment and cell-to-cell aggregation are dependent on the coordinated action of adhesin molecules and exopolysaccharides.
format Text
id pubmed-2632755
institution National Center for Biotechnology Information
language English
publishDate 2009
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-26327552009-02-04 A Filamentous Hemagglutinin-Like Protein of Xanthomonas axonopodis pv. citri, the Phytopathogen Responsible for Citrus Canker, Is Involved in Bacterial Virulence Gottig, Natalia Garavaglia, Betiana S. Garofalo, Cecilia G. Orellano, Elena G. Ottado, Jorgelina PLoS One Research Article Xanthomonas axonopodis pv. citri, the phytopathogen responsible for citrus canker has a number of protein secretion systems and among them, at least one type V protein secretion system belonging to the two-partner secretion pathway. This system is mainly associated to the translocation of large proteins such as adhesins to the outer membrane of several pathogens. Xanthomonas axonopodis pv. citri possess a filamentous hemagglutinin-like protein in close vicinity to its putative transporter protein, XacFhaB and XacFhaC, respectively. Expression analysis indicated that XacFhaB was induced in planta during plant-pathogen interaction. By mutation analysis of XacFhaB and XacFhaC genes we determined that XacFhaB is involved in virulence both in epiphytic and wound inoculations, displaying more dispersed and fewer canker lesions. Unexpectedly, the XacFhaC mutant in the transporter protein produced an intermediate virulence phenotype resembling wild type infection, suggesting that XacFhaB could be secreted by another partner different from XacFhaC. Moreover, XacFhaB mutants showed a general lack of adhesion and were affected in leaf surface attachment and biofilm formation. In agreement with the in planta phenotype, adhesin lacking cells moved faster in swarming plates. Since no hyperflagellation phenotype was observed in this bacteria, the faster movement may be attributed to the lack of cell-to-cell aggregation. Moreover, XacFhaB mutants secreted more exopolysaccharide that in turn may facilitate its motility. Our results suggest that this hemagglutinin-like protein is required for tissue colonization being mainly involved in surface attachment and biofilm formation, and that plant tissue attachment and cell-to-cell aggregation are dependent on the coordinated action of adhesin molecules and exopolysaccharides. Public Library of Science 2009-02-04 /pmc/articles/PMC2632755/ /pubmed/19194503 http://dx.doi.org/10.1371/journal.pone.0004358 Text en Gottig et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Gottig, Natalia
Garavaglia, Betiana S.
Garofalo, Cecilia G.
Orellano, Elena G.
Ottado, Jorgelina
A Filamentous Hemagglutinin-Like Protein of Xanthomonas axonopodis pv. citri, the Phytopathogen Responsible for Citrus Canker, Is Involved in Bacterial Virulence
title A Filamentous Hemagglutinin-Like Protein of Xanthomonas axonopodis pv. citri, the Phytopathogen Responsible for Citrus Canker, Is Involved in Bacterial Virulence
title_full A Filamentous Hemagglutinin-Like Protein of Xanthomonas axonopodis pv. citri, the Phytopathogen Responsible for Citrus Canker, Is Involved in Bacterial Virulence
title_fullStr A Filamentous Hemagglutinin-Like Protein of Xanthomonas axonopodis pv. citri, the Phytopathogen Responsible for Citrus Canker, Is Involved in Bacterial Virulence
title_full_unstemmed A Filamentous Hemagglutinin-Like Protein of Xanthomonas axonopodis pv. citri, the Phytopathogen Responsible for Citrus Canker, Is Involved in Bacterial Virulence
title_short A Filamentous Hemagglutinin-Like Protein of Xanthomonas axonopodis pv. citri, the Phytopathogen Responsible for Citrus Canker, Is Involved in Bacterial Virulence
title_sort filamentous hemagglutinin-like protein of xanthomonas axonopodis pv. citri, the phytopathogen responsible for citrus canker, is involved in bacterial virulence
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2632755/
https://www.ncbi.nlm.nih.gov/pubmed/19194503
http://dx.doi.org/10.1371/journal.pone.0004358
work_keys_str_mv AT gottignatalia afilamentoushemagglutininlikeproteinofxanthomonasaxonopodispvcitrithephytopathogenresponsibleforcitruscankerisinvolvedinbacterialvirulence
AT garavagliabetianas afilamentoushemagglutininlikeproteinofxanthomonasaxonopodispvcitrithephytopathogenresponsibleforcitruscankerisinvolvedinbacterialvirulence
AT garofaloceciliag afilamentoushemagglutininlikeproteinofxanthomonasaxonopodispvcitrithephytopathogenresponsibleforcitruscankerisinvolvedinbacterialvirulence
AT orellanoelenag afilamentoushemagglutininlikeproteinofxanthomonasaxonopodispvcitrithephytopathogenresponsibleforcitruscankerisinvolvedinbacterialvirulence
AT ottadojorgelina afilamentoushemagglutininlikeproteinofxanthomonasaxonopodispvcitrithephytopathogenresponsibleforcitruscankerisinvolvedinbacterialvirulence
AT gottignatalia filamentoushemagglutininlikeproteinofxanthomonasaxonopodispvcitrithephytopathogenresponsibleforcitruscankerisinvolvedinbacterialvirulence
AT garavagliabetianas filamentoushemagglutininlikeproteinofxanthomonasaxonopodispvcitrithephytopathogenresponsibleforcitruscankerisinvolvedinbacterialvirulence
AT garofaloceciliag filamentoushemagglutininlikeproteinofxanthomonasaxonopodispvcitrithephytopathogenresponsibleforcitruscankerisinvolvedinbacterialvirulence
AT orellanoelenag filamentoushemagglutininlikeproteinofxanthomonasaxonopodispvcitrithephytopathogenresponsibleforcitruscankerisinvolvedinbacterialvirulence
AT ottadojorgelina filamentoushemagglutininlikeproteinofxanthomonasaxonopodispvcitrithephytopathogenresponsibleforcitruscankerisinvolvedinbacterialvirulence