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Non-Sterilized Fermentative Production of Polymer-Grade L-Lactic Acid by a Newly Isolated Thermophilic Strain Bacillus sp. 2–6
BACKGROUND: The demand for lactic acid has been increasing considerably because of its use as a monomer for the synthesis of polylactic acid (PLA), which is a promising and environment-friendly alternative to plastics derived from petrochemicals. Optically pure l-lactic acid is essential for polymer...
Autores principales: | , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2009
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2632756/ https://www.ncbi.nlm.nih.gov/pubmed/19194504 http://dx.doi.org/10.1371/journal.pone.0004359 |
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author | Qin, Jiayang Zhao, Bo Wang, Xiuwen Wang, Limin Yu, Bo Ma, Yanhe Ma, Cuiqing Tang, Hongzhi Sun, Jibin Xu, Ping |
author_facet | Qin, Jiayang Zhao, Bo Wang, Xiuwen Wang, Limin Yu, Bo Ma, Yanhe Ma, Cuiqing Tang, Hongzhi Sun, Jibin Xu, Ping |
author_sort | Qin, Jiayang |
collection | PubMed |
description | BACKGROUND: The demand for lactic acid has been increasing considerably because of its use as a monomer for the synthesis of polylactic acid (PLA), which is a promising and environment-friendly alternative to plastics derived from petrochemicals. Optically pure l-lactic acid is essential for polymerization of PLA. The high fermentation cost of l-lactic acid is another limitation for PLA polymers to compete with conventional plastics. METHODOLOGY/PRINCIPAL FINDINGS: A Bacillus sp. strain 2–6 for production of l-lactic acid was isolated at 55°C from soil samples. Its thermophilic characteristic made it a good lactic acid producer because optically pure l-lactic acid could be produced by this strain under open condition without sterilization. In 5-liter batch fermentation of Bacillus sp. 2–6, 118.0 g/liter of l-lactic acid with an optical purity of 99.4% was obtained from 121.3 g/liter of glucose. The yield was 97.3% and the average productivity was 4.37 g/liter/h. The maximum l-lactic acid concentration of 182.0 g/liter was obtained from 30-liter fed-batch fermentation with an average productivity of 3.03 g/liter/h and product optical purity of 99.4%. CONCLUSIONS/SIGNIFICANCE: With the newly isolated Bacillus sp. strain 2–6, high concentration of optically pure l-lactic acid could be produced efficiently in open fermentation without sterilization, which would lead to a new cost-effective method for polymer-grade l-lactic acid production from renewable resources. |
format | Text |
id | pubmed-2632756 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-26327562009-02-04 Non-Sterilized Fermentative Production of Polymer-Grade L-Lactic Acid by a Newly Isolated Thermophilic Strain Bacillus sp. 2–6 Qin, Jiayang Zhao, Bo Wang, Xiuwen Wang, Limin Yu, Bo Ma, Yanhe Ma, Cuiqing Tang, Hongzhi Sun, Jibin Xu, Ping PLoS One Research Article BACKGROUND: The demand for lactic acid has been increasing considerably because of its use as a monomer for the synthesis of polylactic acid (PLA), which is a promising and environment-friendly alternative to plastics derived from petrochemicals. Optically pure l-lactic acid is essential for polymerization of PLA. The high fermentation cost of l-lactic acid is another limitation for PLA polymers to compete with conventional plastics. METHODOLOGY/PRINCIPAL FINDINGS: A Bacillus sp. strain 2–6 for production of l-lactic acid was isolated at 55°C from soil samples. Its thermophilic characteristic made it a good lactic acid producer because optically pure l-lactic acid could be produced by this strain under open condition without sterilization. In 5-liter batch fermentation of Bacillus sp. 2–6, 118.0 g/liter of l-lactic acid with an optical purity of 99.4% was obtained from 121.3 g/liter of glucose. The yield was 97.3% and the average productivity was 4.37 g/liter/h. The maximum l-lactic acid concentration of 182.0 g/liter was obtained from 30-liter fed-batch fermentation with an average productivity of 3.03 g/liter/h and product optical purity of 99.4%. CONCLUSIONS/SIGNIFICANCE: With the newly isolated Bacillus sp. strain 2–6, high concentration of optically pure l-lactic acid could be produced efficiently in open fermentation without sterilization, which would lead to a new cost-effective method for polymer-grade l-lactic acid production from renewable resources. Public Library of Science 2009-02-04 /pmc/articles/PMC2632756/ /pubmed/19194504 http://dx.doi.org/10.1371/journal.pone.0004359 Text en Qin et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Qin, Jiayang Zhao, Bo Wang, Xiuwen Wang, Limin Yu, Bo Ma, Yanhe Ma, Cuiqing Tang, Hongzhi Sun, Jibin Xu, Ping Non-Sterilized Fermentative Production of Polymer-Grade L-Lactic Acid by a Newly Isolated Thermophilic Strain Bacillus sp. 2–6 |
title | Non-Sterilized Fermentative Production of Polymer-Grade L-Lactic Acid by a Newly Isolated Thermophilic Strain Bacillus sp. 2–6 |
title_full | Non-Sterilized Fermentative Production of Polymer-Grade L-Lactic Acid by a Newly Isolated Thermophilic Strain Bacillus sp. 2–6 |
title_fullStr | Non-Sterilized Fermentative Production of Polymer-Grade L-Lactic Acid by a Newly Isolated Thermophilic Strain Bacillus sp. 2–6 |
title_full_unstemmed | Non-Sterilized Fermentative Production of Polymer-Grade L-Lactic Acid by a Newly Isolated Thermophilic Strain Bacillus sp. 2–6 |
title_short | Non-Sterilized Fermentative Production of Polymer-Grade L-Lactic Acid by a Newly Isolated Thermophilic Strain Bacillus sp. 2–6 |
title_sort | non-sterilized fermentative production of polymer-grade l-lactic acid by a newly isolated thermophilic strain bacillus sp. 2–6 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2632756/ https://www.ncbi.nlm.nih.gov/pubmed/19194504 http://dx.doi.org/10.1371/journal.pone.0004359 |
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