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DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways

The assembly of large recombinant DNA encoding a whole biochemical pathway or genome represents a significant challenge. Here, we report a new method, DNA assembler, which allows the assembly of an entire biochemical pathway in a single step via in vivo homologous recombination in Saccharomyces cere...

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Detalles Bibliográficos
Autores principales:	Shao, Zengyi, Zhao, Hua, Zhao, Huimin
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2009
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2632897/ https://www.ncbi.nlm.nih.gov/pubmed/19074487 http://dx.doi.org/10.1093/nar/gkn991

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