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The UDP-glucosyltransferase multigene family in Bombyx mori

BACKGROUND: Glucosidation plays a major role in the inactivation and excretion of a great variety of both endogenous and exogenous compounds. A class of UDP-glycosyltransferases (UGTs) is involved in this process. Insect UGTs play important roles in several processes, including detoxication of subst...

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Autores principales: Huang, Fei-Fei, Chai, Chun-Li, Zhang, Ze, Liu, Zeng-Hu, Dai, Fang-Yin, Lu, Cheng, Xiang, Zhong-Huai
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2633020/
https://www.ncbi.nlm.nih.gov/pubmed/19038024
http://dx.doi.org/10.1186/1471-2164-9-563
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author Huang, Fei-Fei
Chai, Chun-Li
Zhang, Ze
Liu, Zeng-Hu
Dai, Fang-Yin
Lu, Cheng
Xiang, Zhong-Huai
author_facet Huang, Fei-Fei
Chai, Chun-Li
Zhang, Ze
Liu, Zeng-Hu
Dai, Fang-Yin
Lu, Cheng
Xiang, Zhong-Huai
author_sort Huang, Fei-Fei
collection PubMed
description BACKGROUND: Glucosidation plays a major role in the inactivation and excretion of a great variety of both endogenous and exogenous compounds. A class of UDP-glycosyltransferases (UGTs) is involved in this process. Insect UGTs play important roles in several processes, including detoxication of substrates such as plant allelochemicals, cuticle formation, pigmentation, and olfaction. Identification and characterization of Bombyx mori UGT genes could provide valuable basic information for this important family and explain the detoxication mechanism and other processes in insects. RESULTS: Taking advantage of the newly assembled genome sequence, we performed a genome-wide analysis of the candidate UGT family in the silkworm, B. mori. Based on UGT signature and their similarity to UGT homologs from other organisms, we identified 42 putative silkworm UGT genes. Most of them are clustered on the silkworm chromosomes, with two major clusters on chromosomes 7 and 28, respectively. The phylogenetic analysis of these identified 42 UGT protein sequences revealed five major groups. A comparison of the silkworm UGTs with homologs from other sequenced insect genomes indicated that some UGTs are silkworm-specific genes. The expression patterns of these candidate genes were investigated with known expressed sequence tags (ESTs), microarray data, and RT-PCR method. In total, 36 genes were expressed in tissues examined and showed different patterns of expression profile, indicating that these UGT genes might have different functions. CONCLUSION: B. mori possesses a largest insect UGT gene family characterized to date, including 42 genes. Phylogenetic analysis, genomic organization and expression profiles provide an overview for the silkworm UGTs and facilitate their functional studies in future.
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spelling pubmed-26330202009-01-30 The UDP-glucosyltransferase multigene family in Bombyx mori Huang, Fei-Fei Chai, Chun-Li Zhang, Ze Liu, Zeng-Hu Dai, Fang-Yin Lu, Cheng Xiang, Zhong-Huai BMC Genomics Research Article BACKGROUND: Glucosidation plays a major role in the inactivation and excretion of a great variety of both endogenous and exogenous compounds. A class of UDP-glycosyltransferases (UGTs) is involved in this process. Insect UGTs play important roles in several processes, including detoxication of substrates such as plant allelochemicals, cuticle formation, pigmentation, and olfaction. Identification and characterization of Bombyx mori UGT genes could provide valuable basic information for this important family and explain the detoxication mechanism and other processes in insects. RESULTS: Taking advantage of the newly assembled genome sequence, we performed a genome-wide analysis of the candidate UGT family in the silkworm, B. mori. Based on UGT signature and their similarity to UGT homologs from other organisms, we identified 42 putative silkworm UGT genes. Most of them are clustered on the silkworm chromosomes, with two major clusters on chromosomes 7 and 28, respectively. The phylogenetic analysis of these identified 42 UGT protein sequences revealed five major groups. A comparison of the silkworm UGTs with homologs from other sequenced insect genomes indicated that some UGTs are silkworm-specific genes. The expression patterns of these candidate genes were investigated with known expressed sequence tags (ESTs), microarray data, and RT-PCR method. In total, 36 genes were expressed in tissues examined and showed different patterns of expression profile, indicating that these UGT genes might have different functions. CONCLUSION: B. mori possesses a largest insect UGT gene family characterized to date, including 42 genes. Phylogenetic analysis, genomic organization and expression profiles provide an overview for the silkworm UGTs and facilitate their functional studies in future. BioMed Central 2008-11-27 /pmc/articles/PMC2633020/ /pubmed/19038024 http://dx.doi.org/10.1186/1471-2164-9-563 Text en Copyright © 2008 Huang et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Huang, Fei-Fei
Chai, Chun-Li
Zhang, Ze
Liu, Zeng-Hu
Dai, Fang-Yin
Lu, Cheng
Xiang, Zhong-Huai
The UDP-glucosyltransferase multigene family in Bombyx mori
title The UDP-glucosyltransferase multigene family in Bombyx mori
title_full The UDP-glucosyltransferase multigene family in Bombyx mori
title_fullStr The UDP-glucosyltransferase multigene family in Bombyx mori
title_full_unstemmed The UDP-glucosyltransferase multigene family in Bombyx mori
title_short The UDP-glucosyltransferase multigene family in Bombyx mori
title_sort udp-glucosyltransferase multigene family in bombyx mori
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2633020/
https://www.ncbi.nlm.nih.gov/pubmed/19038024
http://dx.doi.org/10.1186/1471-2164-9-563
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