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The Effect of Lactic Acid Bacteria Isolates on the Urinary Tract Pathogens to Infants In Vitro

Urinary tract infections are common clinical problems in children, even though lots of treatment strategies have been tried. Many studies of the application of probiotics for urinary tract infection in female adults exist, but there is a lack of studies in children. The aims of this study were to sc...

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Detalles Bibliográficos
Autores principales: Lim, In Seok, Lee, Ho Seok, Kim, Won Yong
Formato: Texto
Lenguaje:English
Publicado: The Korean Academy of Medical Sciences 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2633189/
https://www.ncbi.nlm.nih.gov/pubmed/19194563
http://dx.doi.org/10.3346/jkms.2009.24.S1.S57
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author Lim, In Seok
Lee, Ho Seok
Kim, Won Yong
author_facet Lim, In Seok
Lee, Ho Seok
Kim, Won Yong
author_sort Lim, In Seok
collection PubMed
description Urinary tract infections are common clinical problems in children, even though lots of treatment strategies have been tried. Many studies of the application of probiotics for urinary tract infection in female adults exist, but there is a lack of studies in children. The aims of this study were to screen probiotic strains for inhibiting the uropathogens in vitro, to find candidates for in vivo study. Nine strains of E. coli were isolated from children with urinary tract infection and six uropathogens were obtained from Korean Colletion for Type Cultures and American Type Culture Collection. Also 135 lactic acid bacteria (LAB) strains were isolated from healthy children, and were identified through physiologic, biochemical methods, 16S rDNA PCR, and data analysis. And with agar disk diffusion assay technique the antimicrobial activities of these LAB strains against those uropathogens were examined. Three strains of separated LAB strains demonstrated major antimicrobial activity against all the uropathogens. In the agar disk diffusion assay technique, antimicrobial activities increased most in the 4th day culture broth with separated Lactobacillus. In summary, some LAB can be used as candidates to develop the probiotic microorganisms that inhibit uropathogens in children, and are expected to be applied to treatment and prevention of pediatric urinary tract infection.
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spelling pubmed-26331892009-02-03 The Effect of Lactic Acid Bacteria Isolates on the Urinary Tract Pathogens to Infants In Vitro Lim, In Seok Lee, Ho Seok Kim, Won Yong J Korean Med Sci Original Article Urinary tract infections are common clinical problems in children, even though lots of treatment strategies have been tried. Many studies of the application of probiotics for urinary tract infection in female adults exist, but there is a lack of studies in children. The aims of this study were to screen probiotic strains for inhibiting the uropathogens in vitro, to find candidates for in vivo study. Nine strains of E. coli were isolated from children with urinary tract infection and six uropathogens were obtained from Korean Colletion for Type Cultures and American Type Culture Collection. Also 135 lactic acid bacteria (LAB) strains were isolated from healthy children, and were identified through physiologic, biochemical methods, 16S rDNA PCR, and data analysis. And with agar disk diffusion assay technique the antimicrobial activities of these LAB strains against those uropathogens were examined. Three strains of separated LAB strains demonstrated major antimicrobial activity against all the uropathogens. In the agar disk diffusion assay technique, antimicrobial activities increased most in the 4th day culture broth with separated Lactobacillus. In summary, some LAB can be used as candidates to develop the probiotic microorganisms that inhibit uropathogens in children, and are expected to be applied to treatment and prevention of pediatric urinary tract infection. The Korean Academy of Medical Sciences 2009-01 2009-01-28 /pmc/articles/PMC2633189/ /pubmed/19194563 http://dx.doi.org/10.3346/jkms.2009.24.S1.S57 Text en Copyright © 2009 The Korean Academy of Medical Sciences http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Lim, In Seok
Lee, Ho Seok
Kim, Won Yong
The Effect of Lactic Acid Bacteria Isolates on the Urinary Tract Pathogens to Infants In Vitro
title The Effect of Lactic Acid Bacteria Isolates on the Urinary Tract Pathogens to Infants In Vitro
title_full The Effect of Lactic Acid Bacteria Isolates on the Urinary Tract Pathogens to Infants In Vitro
title_fullStr The Effect of Lactic Acid Bacteria Isolates on the Urinary Tract Pathogens to Infants In Vitro
title_full_unstemmed The Effect of Lactic Acid Bacteria Isolates on the Urinary Tract Pathogens to Infants In Vitro
title_short The Effect of Lactic Acid Bacteria Isolates on the Urinary Tract Pathogens to Infants In Vitro
title_sort effect of lactic acid bacteria isolates on the urinary tract pathogens to infants in vitro
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2633189/
https://www.ncbi.nlm.nih.gov/pubmed/19194563
http://dx.doi.org/10.3346/jkms.2009.24.S1.S57
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