Cargando…

Clinical Significance of Monitoring Circulating CD4(+)CD25(+) Regulatory T Cells in Kidney Transplantation during the Early Posttransplant Period

The CD4(+)CD25(+) T regulatory cells (Tregs) play an important role in immune tolerance in experimental transplantation but the clinical significance of circulating Tregs in the peripheral blood is undetermined. In 50 kidney transplant (KT) recipients, 29 healthy controls and 32 liver transplant (LT...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Su Hyun, Oh, Eun-Jee, Ghee, Jung Yeon, Song, Hyun Kuk, Han, Dong He, Yoon, Hye Eun, Choi, Bum Soon, Yoon, Seung Kew, Choi, Jong Young, Moon, In Sung, Kim, Dong Goo, Yang, Chul Woo
Formato: Texto
Lenguaje:English
Publicado: The Korean Academy of Medical Sciences 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2633210/
https://www.ncbi.nlm.nih.gov/pubmed/19194543
http://dx.doi.org/10.3346/jkms.2009.24.S1.S135
_version_ 1782164090354925568
author Kim, Su Hyun
Oh, Eun-Jee
Ghee, Jung Yeon
Song, Hyun Kuk
Han, Dong He
Yoon, Hye Eun
Choi, Bum Soon
Yoon, Seung Kew
Choi, Jong Young
Moon, In Sung
Kim, Dong Goo
Yang, Chul Woo
author_facet Kim, Su Hyun
Oh, Eun-Jee
Ghee, Jung Yeon
Song, Hyun Kuk
Han, Dong He
Yoon, Hye Eun
Choi, Bum Soon
Yoon, Seung Kew
Choi, Jong Young
Moon, In Sung
Kim, Dong Goo
Yang, Chul Woo
author_sort Kim, Su Hyun
collection PubMed
description The CD4(+)CD25(+) T regulatory cells (Tregs) play an important role in immune tolerance in experimental transplantation but the clinical significance of circulating Tregs in the peripheral blood is undetermined. In 50 kidney transplant (KT) recipients, 29 healthy controls and 32 liver transplant (LT) recipients, the frequency of Tregs was measured with flow cytometry before and after transplantation. In the KT recipients, IL-10 secretion was measured with an enzyme-linked immunospot (ELISPOT) assay. The median frequency of circulating Tregs before KT was similar to that in healthy controls but significantly lower than that in LT patients before transplantation. The frequency of Tregs was significantly decreased in patients with subclinical acute rejection compared with those without subclinical acute rejection. Calcineurin inhibitors (CNIs) and anti-CD25 antibody decreased the frequency of Tregs but mTOR inhibitor did not. The frequency of donor-specific IL-10 secreting cells did not correlate with the number of Tregs. The frequency of circulating Tregs in KT recipients is strongly affected by CNIs and anti-CD25 antibody, and a low frequency of Tregs is associated with subclinical acute rejection during the early posttransplant period.
format Text
id pubmed-2633210
institution National Center for Biotechnology Information
language English
publishDate 2009
publisher The Korean Academy of Medical Sciences
record_format MEDLINE/PubMed
spelling pubmed-26332102009-02-03 Clinical Significance of Monitoring Circulating CD4(+)CD25(+) Regulatory T Cells in Kidney Transplantation during the Early Posttransplant Period Kim, Su Hyun Oh, Eun-Jee Ghee, Jung Yeon Song, Hyun Kuk Han, Dong He Yoon, Hye Eun Choi, Bum Soon Yoon, Seung Kew Choi, Jong Young Moon, In Sung Kim, Dong Goo Yang, Chul Woo J Korean Med Sci Original Article The CD4(+)CD25(+) T regulatory cells (Tregs) play an important role in immune tolerance in experimental transplantation but the clinical significance of circulating Tregs in the peripheral blood is undetermined. In 50 kidney transplant (KT) recipients, 29 healthy controls and 32 liver transplant (LT) recipients, the frequency of Tregs was measured with flow cytometry before and after transplantation. In the KT recipients, IL-10 secretion was measured with an enzyme-linked immunospot (ELISPOT) assay. The median frequency of circulating Tregs before KT was similar to that in healthy controls but significantly lower than that in LT patients before transplantation. The frequency of Tregs was significantly decreased in patients with subclinical acute rejection compared with those without subclinical acute rejection. Calcineurin inhibitors (CNIs) and anti-CD25 antibody decreased the frequency of Tregs but mTOR inhibitor did not. The frequency of donor-specific IL-10 secreting cells did not correlate with the number of Tregs. The frequency of circulating Tregs in KT recipients is strongly affected by CNIs and anti-CD25 antibody, and a low frequency of Tregs is associated with subclinical acute rejection during the early posttransplant period. The Korean Academy of Medical Sciences 2009-01 2009-01-28 /pmc/articles/PMC2633210/ /pubmed/19194543 http://dx.doi.org/10.3346/jkms.2009.24.S1.S135 Text en Copyright © 2009 The Korean Academy of Medical Sciences http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kim, Su Hyun
Oh, Eun-Jee
Ghee, Jung Yeon
Song, Hyun Kuk
Han, Dong He
Yoon, Hye Eun
Choi, Bum Soon
Yoon, Seung Kew
Choi, Jong Young
Moon, In Sung
Kim, Dong Goo
Yang, Chul Woo
Clinical Significance of Monitoring Circulating CD4(+)CD25(+) Regulatory T Cells in Kidney Transplantation during the Early Posttransplant Period
title Clinical Significance of Monitoring Circulating CD4(+)CD25(+) Regulatory T Cells in Kidney Transplantation during the Early Posttransplant Period
title_full Clinical Significance of Monitoring Circulating CD4(+)CD25(+) Regulatory T Cells in Kidney Transplantation during the Early Posttransplant Period
title_fullStr Clinical Significance of Monitoring Circulating CD4(+)CD25(+) Regulatory T Cells in Kidney Transplantation during the Early Posttransplant Period
title_full_unstemmed Clinical Significance of Monitoring Circulating CD4(+)CD25(+) Regulatory T Cells in Kidney Transplantation during the Early Posttransplant Period
title_short Clinical Significance of Monitoring Circulating CD4(+)CD25(+) Regulatory T Cells in Kidney Transplantation during the Early Posttransplant Period
title_sort clinical significance of monitoring circulating cd4(+)cd25(+) regulatory t cells in kidney transplantation during the early posttransplant period
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2633210/
https://www.ncbi.nlm.nih.gov/pubmed/19194543
http://dx.doi.org/10.3346/jkms.2009.24.S1.S135
work_keys_str_mv AT kimsuhyun clinicalsignificanceofmonitoringcirculatingcd4cd25regulatorytcellsinkidneytransplantationduringtheearlyposttransplantperiod
AT oheunjee clinicalsignificanceofmonitoringcirculatingcd4cd25regulatorytcellsinkidneytransplantationduringtheearlyposttransplantperiod
AT gheejungyeon clinicalsignificanceofmonitoringcirculatingcd4cd25regulatorytcellsinkidneytransplantationduringtheearlyposttransplantperiod
AT songhyunkuk clinicalsignificanceofmonitoringcirculatingcd4cd25regulatorytcellsinkidneytransplantationduringtheearlyposttransplantperiod
AT handonghe clinicalsignificanceofmonitoringcirculatingcd4cd25regulatorytcellsinkidneytransplantationduringtheearlyposttransplantperiod
AT yoonhyeeun clinicalsignificanceofmonitoringcirculatingcd4cd25regulatorytcellsinkidneytransplantationduringtheearlyposttransplantperiod
AT choibumsoon clinicalsignificanceofmonitoringcirculatingcd4cd25regulatorytcellsinkidneytransplantationduringtheearlyposttransplantperiod
AT yoonseungkew clinicalsignificanceofmonitoringcirculatingcd4cd25regulatorytcellsinkidneytransplantationduringtheearlyposttransplantperiod
AT choijongyoung clinicalsignificanceofmonitoringcirculatingcd4cd25regulatorytcellsinkidneytransplantationduringtheearlyposttransplantperiod
AT mooninsung clinicalsignificanceofmonitoringcirculatingcd4cd25regulatorytcellsinkidneytransplantationduringtheearlyposttransplantperiod
AT kimdonggoo clinicalsignificanceofmonitoringcirculatingcd4cd25regulatorytcellsinkidneytransplantationduringtheearlyposttransplantperiod
AT yangchulwoo clinicalsignificanceofmonitoringcirculatingcd4cd25regulatorytcellsinkidneytransplantationduringtheearlyposttransplantperiod