Cargando…

High Data Output and Automated 3D Correlative Light–Electron Microscopy Method

Correlative light/electron microscopy (CLEM) allows the simultaneous observation of a given subcellular structure by fluorescence light microscopy (FLM) and electron microscopy. The use of this approach is becoming increasingly frequent in cell biology. In this study, we report on a new high data ou...

Descripción completa

Detalles Bibliográficos
Autores principales:	Vicidomini, Giuseppe, Gagliani, Maria C, Canfora, Michela, Cortese, Katia, Frosi, Fabio, Santangelo, Clara, Di Fiore, Pier Paolo, Boccacci, Patrizia, Diaspro, Alberto, Tacchetti, Carlo
Formato:	Texto
Lenguaje:	English
Publicado:	Blackwell Publishing Ltd 2008
Materias:	Toolbox
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2635477/ https://www.ncbi.nlm.nih.gov/pubmed/18817522 http://dx.doi.org/10.1111/j.1600-0854.2008.00815.x

Ejemplares similares

Correlative light and electron microscopy imaging of autophagy in a zebrafish infection model
por: Hosseini, Rohola, et al.
Publicado: (2014)

An optimized protocol for immuno-electron microscopy of endogenous LC3
por: De Mazière, Ann, et al.
Publicado: (2022)

Correlative single‐molecule localization microscopy and electron tomography reveals endosome nanoscale domains
por: Franke, Christian, et al.
Publicado: (2019)

Semi-Automated Analysis of Organelle Movement and Membrane Content: Understanding Rab-Motor Complex Transport Function
por: Hume, Alistair N, et al.
Publicado: (2011)

Automated flow control of a multi-lane swimming chamber for small fishes indicates species-specific sensitivity to experimental protocols
por: Illing, Björn, et al.
Publicado: (2021)

Quantitative analysis of autophagic flux by confocal pH-imaging of autophagic intermediates
por: Maulucci, Giuseppe, et al.
Publicado: (2015)

Using novel methodologies to examine the impact of artificial light at night on the cortisol stress response in dispersing Atlantic salmon (Salmo salar L.) fry
por: Newman, Rhian C., et al.
Publicado: (2015)

A high-throughput FRET-based assay for determination of Atg4 activity
por: Li, Min, et al.
Publicado: (2012)

A novel method for autophagy detection in primary cells: Impaired levels of macroautophagy in immunosenescent T cells
por: Phadwal, Kanchan, et al.
Publicado: (2012)

Live imaging and single-cell analysis reveal differential dynamics of autophagy and apoptosis
por: Xu, Yangqing, et al.
Publicado: (2013)

MitoTimer probe reveals the impact of autophagy, fusion, and motility on subcellular distribution of young and old mitochondrial protein and on relative mitochondrial protein age
por: Ferree, Andrew W, et al.
Publicado: (2013)

Dissecting Functions of the Conserved Oligomeric Golgi Tethering Complex Using a Cell-Free Assay
por: Cottam, Nathanael P, et al.
Publicado: (2014)

Protein Ligation in Living Cells Using Sortase
por: Strijbis, Karin, et al.
Publicado: (2012)

A rapid and high content assay that measures cyto-ID-stained autophagic compartments and estimates autophagy flux with potential clinical applications
por: Guo, Sujuan, et al.
Publicado: (2015)

Development and application of an antibody-based protein microarray to assess physiological stress in grizzly bears (Ursus arctos)
por: Carlson, Ruth I., et al.
Publicado: (2016)

Shotgun proteomics as a viable approach for biological discovery in the Pacific oyster
por: Timmins-Schiffman, Emma, et al.
Publicado: (2013)

Enzyme immunoassays as a method for quantifying hair reproductive hormones in two felid species
por: Terwissen, C. V., et al.
Publicado: (2014)

A practical field extraction method for non-invasive monitoring of hormone activity in the black rhinoceros
por: Edwards, Katie L., et al.
Publicado: (2014)

Effects of natural environmental conditions on faecal glucocorticoid metabolite concentrations in jaguars (Panthera onca) in Belize
por: Mesa-Cruz, J. Bernardo, et al.
Publicado: (2014)

Measurement of free glucocorticoids: quantifying corticosteroid-binding globulin binding affinity and its variation within and among mammalian species
por: Delehanty, Brendan, et al.
Publicado: (2015)

Developing a new research tool for use in free-ranging cetaceans: recovering cortisol from harbour porpoise skin
por: Bechshoft, Thea, et al.
Publicado: (2015)

Longitudinal progesterone profiles in baleen from female North Atlantic right whales (Eubalaena glacialis) match known calving history
por: Hunt, Kathleen E., et al.
Publicado: (2016)

Calibration of the HemoCue point-of-care analyser for determining haemoglobin concentration in a lizard and a fish
por: Andrewartha, Sarah J., et al.
Publicado: (2016)

Baleen hormones: a novel tool for retrospective assessment of stress and reproduction in bowhead whales (Balaena mysticetus)
por: Hunt, Kathleen E., et al.
Publicado: (2014)

Temporal overlap and repeatability of feather corticosterone levels: practical considerations for use as a biomarker
por: Harris, Christopher M., et al.
Publicado: (2016)

An evaluation of the use of pentosidine as a biomarker for ageing turtles
por: Iverson, John B., et al.
Publicado: (2017)

Effects of post-mortem storage conditions of bovine epididymides on sperm characteristics: investigating a tool for preservation of sperm from endangered species
por: Strand, Julie, et al.
Publicado: (2016)

Evaluating the effect of sample type on American alligator (Alligator mississippiensis) analyte values in a point-of-care blood analyser
por: Hamilton, Matthew T., et al.
Publicado: (2016)

Using HHsearch to tackle proteins of unknown function: A pilot study with PH domains
por: Fidler, David R., et al.
Publicado: (2016)

Three‐dimensional immersive virtual reality for studying cellular compartments in 3D models from EM preparations of neural tissues
por: Calì, Corrado, et al.
Publicado: (2016)

Get the most out of blow hormones: validation of sampling materials, field storage and extraction techniques for whale respiratory vapour samples
por: Burgess, Elizabeth A., et al.
Publicado: (2016)

Brain maps 4.0—Structure of the rat brain: An open access atlas with global nervous system nomenclature ontology and flatmaps
por: Swanson, Larry W.
Publicado: (2018)

Semen collection and ejaculate characteristics of the Leopard Tortoise (Stigmochelys pardalis)
por: Zimmerman, Dawn M, et al.
Publicado: (2017)

Multiple steroid and thyroid hormones detected in baleen from eight whale species
por: Hunt, Kathleen E, et al.
Publicado: (2017)

Within-sample variation in snowshoe hare faecal glucocorticoid metabolite measurements
por: Lafferty, Diana J R, et al.
Publicado: (2017)

Not just fat: investigating the proteome of cetacean blubber tissue
por: Kershaw, Joanna L, et al.
Publicado: (2018)

A novel method for the measurement of glucocorticoids in dermal secretions of amphibians
por: Santymire, R M, et al.
Publicado: (2018)

Using the shuttlebox experimental design to determine temperature preference for juvenile Westslope Cutthroat Trout (Oncorhynchus clarkii lewisi)
por: Macnaughton, Camille J, et al.
Publicado: (2018)

Generation of a sexually mature individual of the Eastern dwarf tree frog, Litoria fallax, from cryopreserved testicular macerates: proof of capacity of cryopreserved sperm derived offspring to complete development
por: Upton, Rose, et al.
Publicado: (2018)

A novel Atg5-shRNA mouse model enables temporal control of Autophagy in vivo
por: Cassidy, Liam D., et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_o1k390u5bfktj77ekf0d3s90ja