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A Novel Co-polymer Based on Hydroxypropyl α-Cyclodextrin Conjugated to Low Molecular Weight Polyethylenimine as an in Vitro Gene Delivery Vector
A novel co-polymer based on 2-hydroxypropyl-α-cyclodextrin cross-linked by low molecular weight polyethylenimine was synthesized as a gene delivery vector. The copolymer could bind and condense DNA tightly. It showed lower cytotoxicity than PEI 25kDa in SK-BR-3 cells. Transfection efficiency was inc...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2635622/ https://www.ncbi.nlm.nih.gov/pubmed/19330075 http://dx.doi.org/10.3390/ijms9112278 |
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author | Huang, Hongliang Yu, Hai Li, Da Liu, Yang Shen, Fenping Zhou, Jun Wang, Qingqing Tang, Guping |
author_facet | Huang, Hongliang Yu, Hai Li, Da Liu, Yang Shen, Fenping Zhou, Jun Wang, Qingqing Tang, Guping |
author_sort | Huang, Hongliang |
collection | PubMed |
description | A novel co-polymer based on 2-hydroxypropyl-α-cyclodextrin cross-linked by low molecular weight polyethylenimine was synthesized as a gene delivery vector. The copolymer could bind and condense DNA tightly. It showed lower cytotoxicity than PEI 25kDa in SK-BR-3 cells. Transfection efficiency was increased over 5.5-fold higher than PEI 25 kDa in SK-BR-3 cells in complete serum medium. It is a potential candidate vector for gene therapy. |
format | Text |
id | pubmed-2635622 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-26356222009-03-25 A Novel Co-polymer Based on Hydroxypropyl α-Cyclodextrin Conjugated to Low Molecular Weight Polyethylenimine as an in Vitro Gene Delivery Vector Huang, Hongliang Yu, Hai Li, Da Liu, Yang Shen, Fenping Zhou, Jun Wang, Qingqing Tang, Guping Int J Mol Sci Article A novel co-polymer based on 2-hydroxypropyl-α-cyclodextrin cross-linked by low molecular weight polyethylenimine was synthesized as a gene delivery vector. The copolymer could bind and condense DNA tightly. It showed lower cytotoxicity than PEI 25kDa in SK-BR-3 cells. Transfection efficiency was increased over 5.5-fold higher than PEI 25 kDa in SK-BR-3 cells in complete serum medium. It is a potential candidate vector for gene therapy. Molecular Diversity Preservation International (MDPI) 2008-11-21 /pmc/articles/PMC2635622/ /pubmed/19330075 http://dx.doi.org/10.3390/ijms9112278 Text en © 2008 by MDPI http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Huang, Hongliang Yu, Hai Li, Da Liu, Yang Shen, Fenping Zhou, Jun Wang, Qingqing Tang, Guping A Novel Co-polymer Based on Hydroxypropyl α-Cyclodextrin Conjugated to Low Molecular Weight Polyethylenimine as an in Vitro Gene Delivery Vector |
title | A Novel Co-polymer Based on Hydroxypropyl α-Cyclodextrin Conjugated to Low Molecular Weight Polyethylenimine as an in Vitro Gene Delivery Vector |
title_full | A Novel Co-polymer Based on Hydroxypropyl α-Cyclodextrin Conjugated to Low Molecular Weight Polyethylenimine as an in Vitro Gene Delivery Vector |
title_fullStr | A Novel Co-polymer Based on Hydroxypropyl α-Cyclodextrin Conjugated to Low Molecular Weight Polyethylenimine as an in Vitro Gene Delivery Vector |
title_full_unstemmed | A Novel Co-polymer Based on Hydroxypropyl α-Cyclodextrin Conjugated to Low Molecular Weight Polyethylenimine as an in Vitro Gene Delivery Vector |
title_short | A Novel Co-polymer Based on Hydroxypropyl α-Cyclodextrin Conjugated to Low Molecular Weight Polyethylenimine as an in Vitro Gene Delivery Vector |
title_sort | novel co-polymer based on hydroxypropyl α-cyclodextrin conjugated to low molecular weight polyethylenimine as an in vitro gene delivery vector |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2635622/ https://www.ncbi.nlm.nih.gov/pubmed/19330075 http://dx.doi.org/10.3390/ijms9112278 |
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