A Novel Co-polymer Based on Hydroxypropyl α-Cyclodextrin Conjugated to Low Molecular Weight Polyethylenimine as an in Vitro Gene Delivery Vector

A novel co-polymer based on 2-hydroxypropyl-α-cyclodextrin cross-linked by low molecular weight polyethylenimine was synthesized as a gene delivery vector. The copolymer could bind and condense DNA tightly. It showed lower cytotoxicity than PEI 25kDa in SK-BR-3 cells. Transfection efficiency was inc...

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Detalles Bibliográficos
Autores principales: Huang, Hongliang, Yu, Hai, Li, Da, Liu, Yang, Shen, Fenping, Zhou, Jun, Wang, Qingqing, Tang, Guping
Formato: Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2008
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2635622/
https://www.ncbi.nlm.nih.gov/pubmed/19330075
http://dx.doi.org/10.3390/ijms9112278
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author Huang, Hongliang
Yu, Hai
Li, Da
Liu, Yang
Shen, Fenping
Zhou, Jun
Wang, Qingqing
Tang, Guping
author_facet Huang, Hongliang
Yu, Hai
Li, Da
Liu, Yang
Shen, Fenping
Zhou, Jun
Wang, Qingqing
Tang, Guping
author_sort Huang, Hongliang
collection PubMed
description A novel co-polymer based on 2-hydroxypropyl-α-cyclodextrin cross-linked by low molecular weight polyethylenimine was synthesized as a gene delivery vector. The copolymer could bind and condense DNA tightly. It showed lower cytotoxicity than PEI 25kDa in SK-BR-3 cells. Transfection efficiency was increased over 5.5-fold higher than PEI 25 kDa in SK-BR-3 cells in complete serum medium. It is a potential candidate vector for gene therapy.
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spelling pubmed-26356222009-03-25 A Novel Co-polymer Based on Hydroxypropyl α-Cyclodextrin Conjugated to Low Molecular Weight Polyethylenimine as an in Vitro Gene Delivery Vector Huang, Hongliang Yu, Hai Li, Da Liu, Yang Shen, Fenping Zhou, Jun Wang, Qingqing Tang, Guping Int J Mol Sci Article A novel co-polymer based on 2-hydroxypropyl-α-cyclodextrin cross-linked by low molecular weight polyethylenimine was synthesized as a gene delivery vector. The copolymer could bind and condense DNA tightly. It showed lower cytotoxicity than PEI 25kDa in SK-BR-3 cells. Transfection efficiency was increased over 5.5-fold higher than PEI 25 kDa in SK-BR-3 cells in complete serum medium. It is a potential candidate vector for gene therapy. Molecular Diversity Preservation International (MDPI) 2008-11-21 /pmc/articles/PMC2635622/ /pubmed/19330075 http://dx.doi.org/10.3390/ijms9112278 Text en © 2008 by MDPI http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Huang, Hongliang
Yu, Hai
Li, Da
Liu, Yang
Shen, Fenping
Zhou, Jun
Wang, Qingqing
Tang, Guping
A Novel Co-polymer Based on Hydroxypropyl α-Cyclodextrin Conjugated to Low Molecular Weight Polyethylenimine as an in Vitro Gene Delivery Vector
title A Novel Co-polymer Based on Hydroxypropyl α-Cyclodextrin Conjugated to Low Molecular Weight Polyethylenimine as an in Vitro Gene Delivery Vector
title_full A Novel Co-polymer Based on Hydroxypropyl α-Cyclodextrin Conjugated to Low Molecular Weight Polyethylenimine as an in Vitro Gene Delivery Vector
title_fullStr A Novel Co-polymer Based on Hydroxypropyl α-Cyclodextrin Conjugated to Low Molecular Weight Polyethylenimine as an in Vitro Gene Delivery Vector
title_full_unstemmed A Novel Co-polymer Based on Hydroxypropyl α-Cyclodextrin Conjugated to Low Molecular Weight Polyethylenimine as an in Vitro Gene Delivery Vector
title_short A Novel Co-polymer Based on Hydroxypropyl α-Cyclodextrin Conjugated to Low Molecular Weight Polyethylenimine as an in Vitro Gene Delivery Vector
title_sort novel co-polymer based on hydroxypropyl α-cyclodextrin conjugated to low molecular weight polyethylenimine as an in vitro gene delivery vector
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2635622/
https://www.ncbi.nlm.nih.gov/pubmed/19330075
http://dx.doi.org/10.3390/ijms9112278
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