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Improving the Stability of Insulin in Solutions Containing Intestinal Proteases in Vitro
Degradation of insulin was studied in this work. Casein and protamine could obviously suppress degradation of insulin by intestinal enzymes, and could protect insulin from degradation by the mechanism of competition and combination with proteolysis enzyme. What is more, co-incubated with HP-β-CD-cas...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2635643/ https://www.ncbi.nlm.nih.gov/pubmed/19330082 http://dx.doi.org/10.3390/ijms9122376 |
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author | Zhang, Liefeng Jiang, Hui Zhu, Wenjie Wu, Lin Song, Lingling Wu, Qiuyan Ren, Yong |
author_facet | Zhang, Liefeng Jiang, Hui Zhu, Wenjie Wu, Lin Song, Lingling Wu, Qiuyan Ren, Yong |
author_sort | Zhang, Liefeng |
collection | PubMed |
description | Degradation of insulin was studied in this work. Casein and protamine could obviously suppress degradation of insulin by intestinal enzymes, and could protect insulin from degradation by the mechanism of competition and combination with proteolysis enzyme. What is more, co-incubated with HP-β-CD-casein or HP-β-CD-protamine, most insulin was protected from degradation by intestinal enzymes. In addition, it was found that the complexation of insulin with HP-β-CD was characterized by UV absorption spectra. These results indicated that HP-β-CD, casein and protamine could offer some positive and useful results, and could protect insulin from degradation during their transit through the intestinal tract. |
format | Text |
id | pubmed-2635643 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-26356432009-03-25 Improving the Stability of Insulin in Solutions Containing Intestinal Proteases in Vitro Zhang, Liefeng Jiang, Hui Zhu, Wenjie Wu, Lin Song, Lingling Wu, Qiuyan Ren, Yong Int J Mol Sci Article Degradation of insulin was studied in this work. Casein and protamine could obviously suppress degradation of insulin by intestinal enzymes, and could protect insulin from degradation by the mechanism of competition and combination with proteolysis enzyme. What is more, co-incubated with HP-β-CD-casein or HP-β-CD-protamine, most insulin was protected from degradation by intestinal enzymes. In addition, it was found that the complexation of insulin with HP-β-CD was characterized by UV absorption spectra. These results indicated that HP-β-CD, casein and protamine could offer some positive and useful results, and could protect insulin from degradation during their transit through the intestinal tract. Molecular Diversity Preservation International (MDPI) 2008-12-01 /pmc/articles/PMC2635643/ /pubmed/19330082 http://dx.doi.org/10.3390/ijms9122376 Text en © 2008 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Zhang, Liefeng Jiang, Hui Zhu, Wenjie Wu, Lin Song, Lingling Wu, Qiuyan Ren, Yong Improving the Stability of Insulin in Solutions Containing Intestinal Proteases in Vitro |
title | Improving the Stability of Insulin in Solutions Containing Intestinal Proteases in Vitro |
title_full | Improving the Stability of Insulin in Solutions Containing Intestinal Proteases in Vitro |
title_fullStr | Improving the Stability of Insulin in Solutions Containing Intestinal Proteases in Vitro |
title_full_unstemmed | Improving the Stability of Insulin in Solutions Containing Intestinal Proteases in Vitro |
title_short | Improving the Stability of Insulin in Solutions Containing Intestinal Proteases in Vitro |
title_sort | improving the stability of insulin in solutions containing intestinal proteases in vitro |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2635643/ https://www.ncbi.nlm.nih.gov/pubmed/19330082 http://dx.doi.org/10.3390/ijms9122376 |
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