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Improving the Stability of Insulin in Solutions Containing Intestinal Proteases in Vitro

Degradation of insulin was studied in this work. Casein and protamine could obviously suppress degradation of insulin by intestinal enzymes, and could protect insulin from degradation by the mechanism of competition and combination with proteolysis enzyme. What is more, co-incubated with HP-β-CD-cas...

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Autores principales: Zhang, Liefeng, Jiang, Hui, Zhu, Wenjie, Wu, Lin, Song, Lingling, Wu, Qiuyan, Ren, Yong
Formato: Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2635643/
https://www.ncbi.nlm.nih.gov/pubmed/19330082
http://dx.doi.org/10.3390/ijms9122376
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author Zhang, Liefeng
Jiang, Hui
Zhu, Wenjie
Wu, Lin
Song, Lingling
Wu, Qiuyan
Ren, Yong
author_facet Zhang, Liefeng
Jiang, Hui
Zhu, Wenjie
Wu, Lin
Song, Lingling
Wu, Qiuyan
Ren, Yong
author_sort Zhang, Liefeng
collection PubMed
description Degradation of insulin was studied in this work. Casein and protamine could obviously suppress degradation of insulin by intestinal enzymes, and could protect insulin from degradation by the mechanism of competition and combination with proteolysis enzyme. What is more, co-incubated with HP-β-CD-casein or HP-β-CD-protamine, most insulin was protected from degradation by intestinal enzymes. In addition, it was found that the complexation of insulin with HP-β-CD was characterized by UV absorption spectra. These results indicated that HP-β-CD, casein and protamine could offer some positive and useful results, and could protect insulin from degradation during their transit through the intestinal tract.
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spelling pubmed-26356432009-03-25 Improving the Stability of Insulin in Solutions Containing Intestinal Proteases in Vitro Zhang, Liefeng Jiang, Hui Zhu, Wenjie Wu, Lin Song, Lingling Wu, Qiuyan Ren, Yong Int J Mol Sci Article Degradation of insulin was studied in this work. Casein and protamine could obviously suppress degradation of insulin by intestinal enzymes, and could protect insulin from degradation by the mechanism of competition and combination with proteolysis enzyme. What is more, co-incubated with HP-β-CD-casein or HP-β-CD-protamine, most insulin was protected from degradation by intestinal enzymes. In addition, it was found that the complexation of insulin with HP-β-CD was characterized by UV absorption spectra. These results indicated that HP-β-CD, casein and protamine could offer some positive and useful results, and could protect insulin from degradation during their transit through the intestinal tract. Molecular Diversity Preservation International (MDPI) 2008-12-01 /pmc/articles/PMC2635643/ /pubmed/19330082 http://dx.doi.org/10.3390/ijms9122376 Text en © 2008 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Zhang, Liefeng
Jiang, Hui
Zhu, Wenjie
Wu, Lin
Song, Lingling
Wu, Qiuyan
Ren, Yong
Improving the Stability of Insulin in Solutions Containing Intestinal Proteases in Vitro
title Improving the Stability of Insulin in Solutions Containing Intestinal Proteases in Vitro
title_full Improving the Stability of Insulin in Solutions Containing Intestinal Proteases in Vitro
title_fullStr Improving the Stability of Insulin in Solutions Containing Intestinal Proteases in Vitro
title_full_unstemmed Improving the Stability of Insulin in Solutions Containing Intestinal Proteases in Vitro
title_short Improving the Stability of Insulin in Solutions Containing Intestinal Proteases in Vitro
title_sort improving the stability of insulin in solutions containing intestinal proteases in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2635643/
https://www.ncbi.nlm.nih.gov/pubmed/19330082
http://dx.doi.org/10.3390/ijms9122376
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