Potential Use of Quantum Dots in Flow Cytometry

QDs may offer significant advantages in environmental and bead-based applications where the target cells need to be discriminated above background fluorescence. We have examined the possible applications of QDs for flow cytometric measurements (FCM) by studying their excitation - emission spectra an...

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Detalles Bibliográficos
Autores principales: Ibáñez-Peral, Raquel, Bergquist, Peter L., Walter, Malcolm R., Gibbs, Moreland, Goldys, Ewa M., Ferrari, Belinda
Formato: Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2008
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2635656/
https://www.ncbi.nlm.nih.gov/pubmed/19330097
http://dx.doi.org/10.3390/ijms9122622
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author Ibáñez-Peral, Raquel
Bergquist, Peter L.
Walter, Malcolm R.
Gibbs, Moreland
Goldys, Ewa M.
Ferrari, Belinda
author_facet Ibáñez-Peral, Raquel
Bergquist, Peter L.
Walter, Malcolm R.
Gibbs, Moreland
Goldys, Ewa M.
Ferrari, Belinda
author_sort Ibáñez-Peral, Raquel
collection PubMed
description QDs may offer significant advantages in environmental and bead-based applications where the target cells need to be discriminated above background fluorescence. We have examined the possible applications of QDs for flow cytometric measurements (FCM) by studying their excitation - emission spectra and their binding to paramagnetic beads. We labelled beads with either QDs or a commonly-used fluorochrome (FITC) and studied their fluorescence intensity by FCM. Flow cytometric comparisons indicated that the minimum fluorophore concentration required for detection of QDs above autofluorescent background was 100-fold less than for FITC.
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spelling pubmed-26356562009-03-25 Potential Use of Quantum Dots in Flow Cytometry Ibáñez-Peral, Raquel Bergquist, Peter L. Walter, Malcolm R. Gibbs, Moreland Goldys, Ewa M. Ferrari, Belinda Int J Mol Sci Article QDs may offer significant advantages in environmental and bead-based applications where the target cells need to be discriminated above background fluorescence. We have examined the possible applications of QDs for flow cytometric measurements (FCM) by studying their excitation - emission spectra and their binding to paramagnetic beads. We labelled beads with either QDs or a commonly-used fluorochrome (FITC) and studied their fluorescence intensity by FCM. Flow cytometric comparisons indicated that the minimum fluorophore concentration required for detection of QDs above autofluorescent background was 100-fold less than for FITC. Molecular Diversity Preservation International (MDPI) 2008-12-17 /pmc/articles/PMC2635656/ /pubmed/19330097 http://dx.doi.org/10.3390/ijms9122622 Text en © 2008 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Ibáñez-Peral, Raquel
Bergquist, Peter L.
Walter, Malcolm R.
Gibbs, Moreland
Goldys, Ewa M.
Ferrari, Belinda
Potential Use of Quantum Dots in Flow Cytometry
title Potential Use of Quantum Dots in Flow Cytometry
title_full Potential Use of Quantum Dots in Flow Cytometry
title_fullStr Potential Use of Quantum Dots in Flow Cytometry
title_full_unstemmed Potential Use of Quantum Dots in Flow Cytometry
title_short Potential Use of Quantum Dots in Flow Cytometry
title_sort potential use of quantum dots in flow cytometry
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2635656/
https://www.ncbi.nlm.nih.gov/pubmed/19330097
http://dx.doi.org/10.3390/ijms9122622
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