A Single-Molecule Study on the Structural Damage of Ultraviolet Radiated DNA

The structural damage of double-stranded DNA under UV radiation was examined using single-molecule fluorescence microscopy. Compared to undamaged DNA, the diffusion coefficient of λ-DNA was significantly increased with 12 min or 20 min of radiation but remained unchanged for 40 min of exposure possi...

Descripción completa

Detalles Bibliográficos
Autores principales: Schallhorn, Kathryn, Kim, Mickey, Ke, Pu Chun
Formato: Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2008
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2635699/
https://www.ncbi.nlm.nih.gov/pubmed/19325771
_version_ 1782164215441653760
author Schallhorn, Kathryn
Kim, Mickey
Ke, Pu Chun
author_facet Schallhorn, Kathryn
Kim, Mickey
Ke, Pu Chun
author_sort Schallhorn, Kathryn
collection PubMed
description The structural damage of double-stranded DNA under UV radiation was examined using single-molecule fluorescence microscopy. Compared to undamaged DNA, the diffusion coefficient of λ-DNA was significantly increased with 12 min or 20 min of radiation but remained unchanged for 40 min of exposure possibly due to strand crosslinking. The structural damage of DNA was further examined using transmission electron microscopy which revealed kinks and sharp bends along the DNA backbone.
format Text
id pubmed-2635699
institution National Center for Biotechnology Information
language English
publishDate 2008
publisher Molecular Diversity Preservation International (MDPI)
record_format MEDLINE/PubMed
spelling pubmed-26356992009-03-25 A Single-Molecule Study on the Structural Damage of Ultraviolet Radiated DNA Schallhorn, Kathryn Kim, Mickey Ke, Pu Chun Int J Mol Sci Communication The structural damage of double-stranded DNA under UV radiation was examined using single-molecule fluorescence microscopy. Compared to undamaged DNA, the diffusion coefficient of λ-DNA was significantly increased with 12 min or 20 min of radiation but remained unchanged for 40 min of exposure possibly due to strand crosslinking. The structural damage of DNA was further examined using transmission electron microscopy which revealed kinks and sharp bends along the DNA backbone. Molecular Diversity Preservation International (MDPI) 2008-04-24 /pmc/articles/PMC2635699/ /pubmed/19325771 Text en © 2008 by MDPI
spellingShingle Communication
Schallhorn, Kathryn
Kim, Mickey
Ke, Pu Chun
A Single-Molecule Study on the Structural Damage of Ultraviolet Radiated DNA
title A Single-Molecule Study on the Structural Damage of Ultraviolet Radiated DNA
title_full A Single-Molecule Study on the Structural Damage of Ultraviolet Radiated DNA
title_fullStr A Single-Molecule Study on the Structural Damage of Ultraviolet Radiated DNA
title_full_unstemmed A Single-Molecule Study on the Structural Damage of Ultraviolet Radiated DNA
title_short A Single-Molecule Study on the Structural Damage of Ultraviolet Radiated DNA
title_sort single-molecule study on the structural damage of ultraviolet radiated dna
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2635699/
https://www.ncbi.nlm.nih.gov/pubmed/19325771
work_keys_str_mv AT schallhornkathryn asinglemoleculestudyonthestructuraldamageofultravioletradiateddna
AT kimmickey asinglemoleculestudyonthestructuraldamageofultravioletradiateddna
AT kepuchun asinglemoleculestudyonthestructuraldamageofultravioletradiateddna
AT schallhornkathryn singlemoleculestudyonthestructuraldamageofultravioletradiateddna
AT kimmickey singlemoleculestudyonthestructuraldamageofultravioletradiateddna
AT kepuchun singlemoleculestudyonthestructuraldamageofultravioletradiateddna

Ejemplares similares