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Purification and Preliminary Crystallographic Analysis of a New Lys49-PLA2 from B. Jararacussu
BjVIII is a new myotoxic Lys49-PLA2 isolated from Bothrops jararacussu venom that exhibits atypical effects on human platelet aggregation. To better understand the mode of action of BjVIII, crystallographic studies were initiated. Two crystal forms were obtained, both containing two molecules in the...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2635704/ https://www.ncbi.nlm.nih.gov/pubmed/19325781 |
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author | dos Santos, Marcelo L. Fagundes, Fábio H. R. Teixeira, Bruno R. F. Toyama, Marcos H. Aparicio, Ricardo |
author_facet | dos Santos, Marcelo L. Fagundes, Fábio H. R. Teixeira, Bruno R. F. Toyama, Marcos H. Aparicio, Ricardo |
author_sort | dos Santos, Marcelo L. |
collection | PubMed |
description | BjVIII is a new myotoxic Lys49-PLA2 isolated from Bothrops jararacussu venom that exhibits atypical effects on human platelet aggregation. To better understand the mode of action of BjVIII, crystallographic studies were initiated. Two crystal forms were obtained, both containing two molecules in the asymmetric unit (ASU). Synchrotron radiation diffraction data were collected to 2.0 Å resolution and 1.9 Å resolution for crystals belonging to the space group P2(1)2(1)2(1) (a = 48.4 Å, b = 65.3 Å, c = 84.3 Å) and space group P3(1)21 (a = b = 55.7 Å, c = 127.9 Å), respectively. Refinement is currently in progress and the refined structures are expected to shed light on the unusual platelet aggregation activity observed for BjVIII. |
format | Text |
id | pubmed-2635704 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-26357042009-03-25 Purification and Preliminary Crystallographic Analysis of a New Lys49-PLA2 from B. Jararacussu dos Santos, Marcelo L. Fagundes, Fábio H. R. Teixeira, Bruno R. F. Toyama, Marcos H. Aparicio, Ricardo Int J Mol Sci Article BjVIII is a new myotoxic Lys49-PLA2 isolated from Bothrops jararacussu venom that exhibits atypical effects on human platelet aggregation. To better understand the mode of action of BjVIII, crystallographic studies were initiated. Two crystal forms were obtained, both containing two molecules in the asymmetric unit (ASU). Synchrotron radiation diffraction data were collected to 2.0 Å resolution and 1.9 Å resolution for crystals belonging to the space group P2(1)2(1)2(1) (a = 48.4 Å, b = 65.3 Å, c = 84.3 Å) and space group P3(1)21 (a = b = 55.7 Å, c = 127.9 Å), respectively. Refinement is currently in progress and the refined structures are expected to shed light on the unusual platelet aggregation activity observed for BjVIII. Molecular Diversity Preservation International (MDPI) 2008-05-08 /pmc/articles/PMC2635704/ /pubmed/19325781 Text en © 2008 by MDPI |
spellingShingle | Article dos Santos, Marcelo L. Fagundes, Fábio H. R. Teixeira, Bruno R. F. Toyama, Marcos H. Aparicio, Ricardo Purification and Preliminary Crystallographic Analysis of a New Lys49-PLA2 from B. Jararacussu |
title | Purification and Preliminary Crystallographic Analysis of a New Lys49-PLA2 from B. Jararacussu |
title_full | Purification and Preliminary Crystallographic Analysis of a New Lys49-PLA2 from B. Jararacussu |
title_fullStr | Purification and Preliminary Crystallographic Analysis of a New Lys49-PLA2 from B. Jararacussu |
title_full_unstemmed | Purification and Preliminary Crystallographic Analysis of a New Lys49-PLA2 from B. Jararacussu |
title_short | Purification and Preliminary Crystallographic Analysis of a New Lys49-PLA2 from B. Jararacussu |
title_sort | purification and preliminary crystallographic analysis of a new lys49-pla2 from b. jararacussu |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2635704/ https://www.ncbi.nlm.nih.gov/pubmed/19325781 |
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