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Purification and Preliminary Crystallographic Analysis of a New Lys49-PLA2 from B. Jararacussu

BjVIII is a new myotoxic Lys49-PLA2 isolated from Bothrops jararacussu venom that exhibits atypical effects on human platelet aggregation. To better understand the mode of action of BjVIII, crystallographic studies were initiated. Two crystal forms were obtained, both containing two molecules in the...

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Autores principales: dos Santos, Marcelo L., Fagundes, Fábio H. R., Teixeira, Bruno R. F., Toyama, Marcos H., Aparicio, Ricardo
Formato: Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2635704/
https://www.ncbi.nlm.nih.gov/pubmed/19325781
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author dos Santos, Marcelo L.
Fagundes, Fábio H. R.
Teixeira, Bruno R. F.
Toyama, Marcos H.
Aparicio, Ricardo
author_facet dos Santos, Marcelo L.
Fagundes, Fábio H. R.
Teixeira, Bruno R. F.
Toyama, Marcos H.
Aparicio, Ricardo
author_sort dos Santos, Marcelo L.
collection PubMed
description BjVIII is a new myotoxic Lys49-PLA2 isolated from Bothrops jararacussu venom that exhibits atypical effects on human platelet aggregation. To better understand the mode of action of BjVIII, crystallographic studies were initiated. Two crystal forms were obtained, both containing two molecules in the asymmetric unit (ASU). Synchrotron radiation diffraction data were collected to 2.0 Å resolution and 1.9 Å resolution for crystals belonging to the space group P2(1)2(1)2(1) (a = 48.4 Å, b = 65.3 Å, c = 84.3 Å) and space group P3(1)21 (a = b = 55.7 Å, c = 127.9 Å), respectively. Refinement is currently in progress and the refined structures are expected to shed light on the unusual platelet aggregation activity observed for BjVIII.
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spelling pubmed-26357042009-03-25 Purification and Preliminary Crystallographic Analysis of a New Lys49-PLA2 from B. Jararacussu dos Santos, Marcelo L. Fagundes, Fábio H. R. Teixeira, Bruno R. F. Toyama, Marcos H. Aparicio, Ricardo Int J Mol Sci Article BjVIII is a new myotoxic Lys49-PLA2 isolated from Bothrops jararacussu venom that exhibits atypical effects on human platelet aggregation. To better understand the mode of action of BjVIII, crystallographic studies were initiated. Two crystal forms were obtained, both containing two molecules in the asymmetric unit (ASU). Synchrotron radiation diffraction data were collected to 2.0 Å resolution and 1.9 Å resolution for crystals belonging to the space group P2(1)2(1)2(1) (a = 48.4 Å, b = 65.3 Å, c = 84.3 Å) and space group P3(1)21 (a = b = 55.7 Å, c = 127.9 Å), respectively. Refinement is currently in progress and the refined structures are expected to shed light on the unusual platelet aggregation activity observed for BjVIII. Molecular Diversity Preservation International (MDPI) 2008-05-08 /pmc/articles/PMC2635704/ /pubmed/19325781 Text en © 2008 by MDPI
spellingShingle Article
dos Santos, Marcelo L.
Fagundes, Fábio H. R.
Teixeira, Bruno R. F.
Toyama, Marcos H.
Aparicio, Ricardo
Purification and Preliminary Crystallographic Analysis of a New Lys49-PLA2 from B. Jararacussu
title Purification and Preliminary Crystallographic Analysis of a New Lys49-PLA2 from B. Jararacussu
title_full Purification and Preliminary Crystallographic Analysis of a New Lys49-PLA2 from B. Jararacussu
title_fullStr Purification and Preliminary Crystallographic Analysis of a New Lys49-PLA2 from B. Jararacussu
title_full_unstemmed Purification and Preliminary Crystallographic Analysis of a New Lys49-PLA2 from B. Jararacussu
title_short Purification and Preliminary Crystallographic Analysis of a New Lys49-PLA2 from B. Jararacussu
title_sort purification and preliminary crystallographic analysis of a new lys49-pla2 from b. jararacussu
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2635704/
https://www.ncbi.nlm.nih.gov/pubmed/19325781
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