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Electrochemical Investigation of Oligonucleotide-DNA Hybridization on Poly(4-Methoxyphenethylamine)
This work describes the immobilization of purine and pyrimidine bases and immobilization/hybridization of synthetic oligonucleotides on graphite electrodes modified with poly(4-methoxyphenethylamine) produced in acid medium. The immobilization of adenine, guanine, cytosine and thymine on these modif...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2635724/ https://www.ncbi.nlm.nih.gov/pubmed/19325797 http://dx.doi.org/10.3390/ijms9071173 |
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author | Silva, Francielle B. Vieira, Sabrina N. Goulart Filho, Luiz R. Boodts, Julien F. C. Brito-Madurro, Ana G. Madurro, João M. |
author_facet | Silva, Francielle B. Vieira, Sabrina N. Goulart Filho, Luiz R. Boodts, Julien F. C. Brito-Madurro, Ana G. Madurro, João M. |
author_sort | Silva, Francielle B. |
collection | PubMed |
description | This work describes the immobilization of purine and pyrimidine bases and immobilization/hybridization of synthetic oligonucleotides on graphite electrodes modified with poly(4-methoxyphenethylamine) produced in acid medium. The immobilization of adenine, guanine, cytosine and thymine on these modified electrodes was efficient, producing characteristic peaks. Another relevant observation is that, according to the literature, pyrimidine bases, cytosine and thymine are more difficult to detect. However, when immobilized onto the poly(4-methoxyphenethylamine), a significant increase in the magnitude of the current was obtained. The observation of the hybridization between the poly(GA) probe and its complementary, poly(CT) target, was possible by monitoring the guanosine and adenosine peaks or through methylene blue indicator, using differential pulse voltammetry. Hybridization results in a decrease of the peak current of guanosine and adenosine or the signal of methylene blue accumulated on the modified electrode surface. The hybridization with the complementary target was also investigated by electrochemical impedance spectroscopy. The results showed a significant modification in the Nyquist plot, after addition of the complementary target, with increase of the charge transference resistance. |
format | Text |
id | pubmed-2635724 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-26357242009-03-25 Electrochemical Investigation of Oligonucleotide-DNA Hybridization on Poly(4-Methoxyphenethylamine) Silva, Francielle B. Vieira, Sabrina N. Goulart Filho, Luiz R. Boodts, Julien F. C. Brito-Madurro, Ana G. Madurro, João M. Int J Mol Sci Article This work describes the immobilization of purine and pyrimidine bases and immobilization/hybridization of synthetic oligonucleotides on graphite electrodes modified with poly(4-methoxyphenethylamine) produced in acid medium. The immobilization of adenine, guanine, cytosine and thymine on these modified electrodes was efficient, producing characteristic peaks. Another relevant observation is that, according to the literature, pyrimidine bases, cytosine and thymine are more difficult to detect. However, when immobilized onto the poly(4-methoxyphenethylamine), a significant increase in the magnitude of the current was obtained. The observation of the hybridization between the poly(GA) probe and its complementary, poly(CT) target, was possible by monitoring the guanosine and adenosine peaks or through methylene blue indicator, using differential pulse voltammetry. Hybridization results in a decrease of the peak current of guanosine and adenosine or the signal of methylene blue accumulated on the modified electrode surface. The hybridization with the complementary target was also investigated by electrochemical impedance spectroscopy. The results showed a significant modification in the Nyquist plot, after addition of the complementary target, with increase of the charge transference resistance. Molecular Diversity Preservation International (MDPI) 2008-07-08 /pmc/articles/PMC2635724/ /pubmed/19325797 http://dx.doi.org/10.3390/ijms9071173 Text en © 2008 by MDPI |
spellingShingle | Article Silva, Francielle B. Vieira, Sabrina N. Goulart Filho, Luiz R. Boodts, Julien F. C. Brito-Madurro, Ana G. Madurro, João M. Electrochemical Investigation of Oligonucleotide-DNA Hybridization on Poly(4-Methoxyphenethylamine) |
title | Electrochemical Investigation of Oligonucleotide-DNA Hybridization on Poly(4-Methoxyphenethylamine) |
title_full | Electrochemical Investigation of Oligonucleotide-DNA Hybridization on Poly(4-Methoxyphenethylamine) |
title_fullStr | Electrochemical Investigation of Oligonucleotide-DNA Hybridization on Poly(4-Methoxyphenethylamine) |
title_full_unstemmed | Electrochemical Investigation of Oligonucleotide-DNA Hybridization on Poly(4-Methoxyphenethylamine) |
title_short | Electrochemical Investigation of Oligonucleotide-DNA Hybridization on Poly(4-Methoxyphenethylamine) |
title_sort | electrochemical investigation of oligonucleotide-dna hybridization on poly(4-methoxyphenethylamine) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2635724/ https://www.ncbi.nlm.nih.gov/pubmed/19325797 http://dx.doi.org/10.3390/ijms9071173 |
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