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Electrochemical Investigation of Oligonucleotide-DNA Hybridization on Poly(4-Methoxyphenethylamine)

This work describes the immobilization of purine and pyrimidine bases and immobilization/hybridization of synthetic oligonucleotides on graphite electrodes modified with poly(4-methoxyphenethylamine) produced in acid medium. The immobilization of adenine, guanine, cytosine and thymine on these modif...

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Autores principales: Silva, Francielle B., Vieira, Sabrina N., Goulart Filho, Luiz R., Boodts, Julien F. C., Brito-Madurro, Ana G., Madurro, João M.
Formato: Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2635724/
https://www.ncbi.nlm.nih.gov/pubmed/19325797
http://dx.doi.org/10.3390/ijms9071173
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author Silva, Francielle B.
Vieira, Sabrina N.
Goulart Filho, Luiz R.
Boodts, Julien F. C.
Brito-Madurro, Ana G.
Madurro, João M.
author_facet Silva, Francielle B.
Vieira, Sabrina N.
Goulart Filho, Luiz R.
Boodts, Julien F. C.
Brito-Madurro, Ana G.
Madurro, João M.
author_sort Silva, Francielle B.
collection PubMed
description This work describes the immobilization of purine and pyrimidine bases and immobilization/hybridization of synthetic oligonucleotides on graphite electrodes modified with poly(4-methoxyphenethylamine) produced in acid medium. The immobilization of adenine, guanine, cytosine and thymine on these modified electrodes was efficient, producing characteristic peaks. Another relevant observation is that, according to the literature, pyrimidine bases, cytosine and thymine are more difficult to detect. However, when immobilized onto the poly(4-methoxyphenethylamine), a significant increase in the magnitude of the current was obtained. The observation of the hybridization between the poly(GA) probe and its complementary, poly(CT) target, was possible by monitoring the guanosine and adenosine peaks or through methylene blue indicator, using differential pulse voltammetry. Hybridization results in a decrease of the peak current of guanosine and adenosine or the signal of methylene blue accumulated on the modified electrode surface. The hybridization with the complementary target was also investigated by electrochemical impedance spectroscopy. The results showed a significant modification in the Nyquist plot, after addition of the complementary target, with increase of the charge transference resistance.
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spelling pubmed-26357242009-03-25 Electrochemical Investigation of Oligonucleotide-DNA Hybridization on Poly(4-Methoxyphenethylamine) Silva, Francielle B. Vieira, Sabrina N. Goulart Filho, Luiz R. Boodts, Julien F. C. Brito-Madurro, Ana G. Madurro, João M. Int J Mol Sci Article This work describes the immobilization of purine and pyrimidine bases and immobilization/hybridization of synthetic oligonucleotides on graphite electrodes modified with poly(4-methoxyphenethylamine) produced in acid medium. The immobilization of adenine, guanine, cytosine and thymine on these modified electrodes was efficient, producing characteristic peaks. Another relevant observation is that, according to the literature, pyrimidine bases, cytosine and thymine are more difficult to detect. However, when immobilized onto the poly(4-methoxyphenethylamine), a significant increase in the magnitude of the current was obtained. The observation of the hybridization between the poly(GA) probe and its complementary, poly(CT) target, was possible by monitoring the guanosine and adenosine peaks or through methylene blue indicator, using differential pulse voltammetry. Hybridization results in a decrease of the peak current of guanosine and adenosine or the signal of methylene blue accumulated on the modified electrode surface. The hybridization with the complementary target was also investigated by electrochemical impedance spectroscopy. The results showed a significant modification in the Nyquist plot, after addition of the complementary target, with increase of the charge transference resistance. Molecular Diversity Preservation International (MDPI) 2008-07-08 /pmc/articles/PMC2635724/ /pubmed/19325797 http://dx.doi.org/10.3390/ijms9071173 Text en © 2008 by MDPI
spellingShingle Article
Silva, Francielle B.
Vieira, Sabrina N.
Goulart Filho, Luiz R.
Boodts, Julien F. C.
Brito-Madurro, Ana G.
Madurro, João M.
Electrochemical Investigation of Oligonucleotide-DNA Hybridization on Poly(4-Methoxyphenethylamine)
title Electrochemical Investigation of Oligonucleotide-DNA Hybridization on Poly(4-Methoxyphenethylamine)
title_full Electrochemical Investigation of Oligonucleotide-DNA Hybridization on Poly(4-Methoxyphenethylamine)
title_fullStr Electrochemical Investigation of Oligonucleotide-DNA Hybridization on Poly(4-Methoxyphenethylamine)
title_full_unstemmed Electrochemical Investigation of Oligonucleotide-DNA Hybridization on Poly(4-Methoxyphenethylamine)
title_short Electrochemical Investigation of Oligonucleotide-DNA Hybridization on Poly(4-Methoxyphenethylamine)
title_sort electrochemical investigation of oligonucleotide-dna hybridization on poly(4-methoxyphenethylamine)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2635724/
https://www.ncbi.nlm.nih.gov/pubmed/19325797
http://dx.doi.org/10.3390/ijms9071173
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