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Microseeding – A Powerful Tool for Crystallizing Proteins Complexed with Hydrolyzable Substrates

Hydrolysis is an often-encountered obstacle in the crystallization of proteins complexed with their substrates. As the duration of the crystallization process, from nucleation to the growth of the crystal to its final size, commonly requires several weeks, non-enzymatic hydrolysis of an “unstable” l...

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Detalles Bibliográficos
Autores principales: Oswald, Christine, Smits, Sander H. J., Bremer, Erhard, Schmitt, Lutz
Formato: Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2635726/
https://www.ncbi.nlm.nih.gov/pubmed/19325794
http://dx.doi.org/10.3390/ijms9071131
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author Oswald, Christine
Smits, Sander H. J.
Bremer, Erhard
Schmitt, Lutz
author_facet Oswald, Christine
Smits, Sander H. J.
Bremer, Erhard
Schmitt, Lutz
author_sort Oswald, Christine
collection PubMed
description Hydrolysis is an often-encountered obstacle in the crystallization of proteins complexed with their substrates. As the duration of the crystallization process, from nucleation to the growth of the crystal to its final size, commonly requires several weeks, non-enzymatic hydrolysis of an “unstable” ligand occurs frequently. In cases where the crystallization conditions exhibit non neutral pH values this hydrolysis phenomenon may be even more pronounced. ChoX, the substrate binding protein of a choline ABC-importer, produced crystals with its substrate acetylcholine after one month. However, these crystals exhibited only choline, an acetylcholine hydrolysis product, in the binding site. To overcome this obstacle we devised a microseeding protocol leading to crystals of ChoX with bound acetylcholine within 24 hours. One drawback we encountered was the high twinning fraction of the crystals, possibly was due to the rapid crystal growth.
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spelling pubmed-26357262009-03-25 Microseeding – A Powerful Tool for Crystallizing Proteins Complexed with Hydrolyzable Substrates Oswald, Christine Smits, Sander H. J. Bremer, Erhard Schmitt, Lutz Int J Mol Sci Article Hydrolysis is an often-encountered obstacle in the crystallization of proteins complexed with their substrates. As the duration of the crystallization process, from nucleation to the growth of the crystal to its final size, commonly requires several weeks, non-enzymatic hydrolysis of an “unstable” ligand occurs frequently. In cases where the crystallization conditions exhibit non neutral pH values this hydrolysis phenomenon may be even more pronounced. ChoX, the substrate binding protein of a choline ABC-importer, produced crystals with its substrate acetylcholine after one month. However, these crystals exhibited only choline, an acetylcholine hydrolysis product, in the binding site. To overcome this obstacle we devised a microseeding protocol leading to crystals of ChoX with bound acetylcholine within 24 hours. One drawback we encountered was the high twinning fraction of the crystals, possibly was due to the rapid crystal growth. Molecular Diversity Preservation International (MDPI) 2008-07-08 /pmc/articles/PMC2635726/ /pubmed/19325794 http://dx.doi.org/10.3390/ijms9071131 Text en © 2008 by MDPI
spellingShingle Article
Oswald, Christine
Smits, Sander H. J.
Bremer, Erhard
Schmitt, Lutz
Microseeding – A Powerful Tool for Crystallizing Proteins Complexed with Hydrolyzable Substrates
title Microseeding – A Powerful Tool for Crystallizing Proteins Complexed with Hydrolyzable Substrates
title_full Microseeding – A Powerful Tool for Crystallizing Proteins Complexed with Hydrolyzable Substrates
title_fullStr Microseeding – A Powerful Tool for Crystallizing Proteins Complexed with Hydrolyzable Substrates
title_full_unstemmed Microseeding – A Powerful Tool for Crystallizing Proteins Complexed with Hydrolyzable Substrates
title_short Microseeding – A Powerful Tool for Crystallizing Proteins Complexed with Hydrolyzable Substrates
title_sort microseeding – a powerful tool for crystallizing proteins complexed with hydrolyzable substrates
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2635726/
https://www.ncbi.nlm.nih.gov/pubmed/19325794
http://dx.doi.org/10.3390/ijms9071131
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