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IrrE, a Global Regulator of Extreme Radiation Resistance in Deinococcus radiodurans, Enhances Salt Tolerance in Escherichia coli and Brassica napus

BACKGROUND: Globally, about 20% of cultivated land is now affected by salinity. Salt tolerance is a trait of importance to all crops in saline soils. Previous efforts to improve salt tolerance in crop plants have met with only limited success. Bacteria of the genus Deinococcus are known for their ab...

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Autores principales: Pan, Jie, Wang, Jin, Zhou, Zhengfu, Yan, Yongliang, Zhang, Wei, Lu, Wei, Ping, Shuzhen, Dai, Qilin, Yuan, Menglong, Feng, Bin, Hou, Xiaoguang, Zhang, Ying, Ruiqiang, Liu, Tingting, Feng, Lu, Wang, Lei, Chen, Ming, Lin, Min
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2635966/
https://www.ncbi.nlm.nih.gov/pubmed/19204796
http://dx.doi.org/10.1371/journal.pone.0004422
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author Pan, Jie
Wang, Jin
Zhou, Zhengfu
Yan, Yongliang
Zhang, Wei
Lu, Wei
Ping, Shuzhen
Dai, Qilin
Yuan, Menglong
Feng, Bin
Hou, Xiaoguang
Zhang, Ying
Ruiqiang,
Liu, Tingting
Feng, Lu
Wang, Lei
Chen, Ming
Lin, Min
author_facet Pan, Jie
Wang, Jin
Zhou, Zhengfu
Yan, Yongliang
Zhang, Wei
Lu, Wei
Ping, Shuzhen
Dai, Qilin
Yuan, Menglong
Feng, Bin
Hou, Xiaoguang
Zhang, Ying
Ruiqiang,
Liu, Tingting
Feng, Lu
Wang, Lei
Chen, Ming
Lin, Min
author_sort Pan, Jie
collection PubMed
description BACKGROUND: Globally, about 20% of cultivated land is now affected by salinity. Salt tolerance is a trait of importance to all crops in saline soils. Previous efforts to improve salt tolerance in crop plants have met with only limited success. Bacteria of the genus Deinococcus are known for their ability to survive highly stressful conditions, and therefore possess a unique pool of genes conferring extreme resistance. In Deinococcus radiodurans, the irrE gene encodes a global regulator responsible for extreme radioresistance. METHODOLOGY/PRINCIPAL FINDINGS: Using plate assays, we showed that IrrE protected E. coli cells against salt shock and other abiotic stresses such as oxidative, osmotic and thermal shocks. Comparative proteomic analysis revealed that IrrE functions as a switch to regulate different sets of proteins such as stress responsive proteins, protein kinases, glycerol-degrading enzymes, detoxification proteins, and growth-related proteins in E. coli. We also used quantitative RT-PCR to investigate expression of nine selected stress-responsive genes in transgenic and wild-type Brassica napus plants. Transgenic B. napus plants expressing the IrrE protein can tolerate 350 mM NaCl, a concentration that inhibits the growth of almost all crop plants. CONCLUSIONS: Expression of IrrE, a global regulator for extreme radiation resistance in D. radiodurans, confers significantly enhanced salt tolerance in both E. coli and B. napus. We thus propose that the irrE gene might be used as a potentially promising transgene to improve abiotic stress tolerances in crop plants.
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spelling pubmed-26359662009-02-10 IrrE, a Global Regulator of Extreme Radiation Resistance in Deinococcus radiodurans, Enhances Salt Tolerance in Escherichia coli and Brassica napus Pan, Jie Wang, Jin Zhou, Zhengfu Yan, Yongliang Zhang, Wei Lu, Wei Ping, Shuzhen Dai, Qilin Yuan, Menglong Feng, Bin Hou, Xiaoguang Zhang, Ying Ruiqiang, Liu, Tingting Feng, Lu Wang, Lei Chen, Ming Lin, Min PLoS One Research Article BACKGROUND: Globally, about 20% of cultivated land is now affected by salinity. Salt tolerance is a trait of importance to all crops in saline soils. Previous efforts to improve salt tolerance in crop plants have met with only limited success. Bacteria of the genus Deinococcus are known for their ability to survive highly stressful conditions, and therefore possess a unique pool of genes conferring extreme resistance. In Deinococcus radiodurans, the irrE gene encodes a global regulator responsible for extreme radioresistance. METHODOLOGY/PRINCIPAL FINDINGS: Using plate assays, we showed that IrrE protected E. coli cells against salt shock and other abiotic stresses such as oxidative, osmotic and thermal shocks. Comparative proteomic analysis revealed that IrrE functions as a switch to regulate different sets of proteins such as stress responsive proteins, protein kinases, glycerol-degrading enzymes, detoxification proteins, and growth-related proteins in E. coli. We also used quantitative RT-PCR to investigate expression of nine selected stress-responsive genes in transgenic and wild-type Brassica napus plants. Transgenic B. napus plants expressing the IrrE protein can tolerate 350 mM NaCl, a concentration that inhibits the growth of almost all crop plants. CONCLUSIONS: Expression of IrrE, a global regulator for extreme radiation resistance in D. radiodurans, confers significantly enhanced salt tolerance in both E. coli and B. napus. We thus propose that the irrE gene might be used as a potentially promising transgene to improve abiotic stress tolerances in crop plants. Public Library of Science 2009-02-10 /pmc/articles/PMC2635966/ /pubmed/19204796 http://dx.doi.org/10.1371/journal.pone.0004422 Text en Pan et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Pan, Jie
Wang, Jin
Zhou, Zhengfu
Yan, Yongliang
Zhang, Wei
Lu, Wei
Ping, Shuzhen
Dai, Qilin
Yuan, Menglong
Feng, Bin
Hou, Xiaoguang
Zhang, Ying
Ruiqiang,
Liu, Tingting
Feng, Lu
Wang, Lei
Chen, Ming
Lin, Min
IrrE, a Global Regulator of Extreme Radiation Resistance in Deinococcus radiodurans, Enhances Salt Tolerance in Escherichia coli and Brassica napus
title IrrE, a Global Regulator of Extreme Radiation Resistance in Deinococcus radiodurans, Enhances Salt Tolerance in Escherichia coli and Brassica napus
title_full IrrE, a Global Regulator of Extreme Radiation Resistance in Deinococcus radiodurans, Enhances Salt Tolerance in Escherichia coli and Brassica napus
title_fullStr IrrE, a Global Regulator of Extreme Radiation Resistance in Deinococcus radiodurans, Enhances Salt Tolerance in Escherichia coli and Brassica napus
title_full_unstemmed IrrE, a Global Regulator of Extreme Radiation Resistance in Deinococcus radiodurans, Enhances Salt Tolerance in Escherichia coli and Brassica napus
title_short IrrE, a Global Regulator of Extreme Radiation Resistance in Deinococcus radiodurans, Enhances Salt Tolerance in Escherichia coli and Brassica napus
title_sort irre, a global regulator of extreme radiation resistance in deinococcus radiodurans, enhances salt tolerance in escherichia coli and brassica napus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2635966/
https://www.ncbi.nlm.nih.gov/pubmed/19204796
http://dx.doi.org/10.1371/journal.pone.0004422
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