Cargando…

Impact of surface electric properties of carbon-based thin films on platelets activation for nano-medical and nano-sensing applications

Electric surface properties of biomaterials, playing key role to various biointerfacial interactions, were related to hemocompatibility and biosensing phenomena. In this study, the examination of surface electric properties of amorphous hydrogenated carbon thin films (a-C:H) was carried out by means...

Descripción completa

Detalles Bibliográficos
Autores principales: Karagkiozaki, Varvara, Logothetidis, Stergios, Lousinian, Sylvie, Giannoglou, George
Formato: Texto
Lenguaje:English
Publicado: Dove Medical Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2636585/
https://www.ncbi.nlm.nih.gov/pubmed/19337414
_version_ 1782164293492408320
author Karagkiozaki, Varvara
Logothetidis, Stergios
Lousinian, Sylvie
Giannoglou, George
author_facet Karagkiozaki, Varvara
Logothetidis, Stergios
Lousinian, Sylvie
Giannoglou, George
author_sort Karagkiozaki, Varvara
collection PubMed
description Electric surface properties of biomaterials, playing key role to various biointerfacial interactions, were related to hemocompatibility and biosensing phenomena. In this study, the examination of surface electric properties of amorphous hydrogenated carbon thin films (a-C:H) was carried out by means of electrostatic force microscope (EFM) and observation of differences in spatial charge distribution on the surface of the examined films during platelets adhesion was made. The thrombogenic potential of a-C:H thin films developed by magnetron sputtering with ~42% sp(3) content and hydrogen partial pressure during deposition was evaluated, by in situ observation with atomic force microscope (AFM) of platelets’ activation and their subsequent adhesion. Platelet-rich plasma drawn from healthy donors was used and semi-contact mode of AFM was applied. Platelets behavior and their correlation with the electric surface properties of the examined a-C:H films by EFM was made for hemocompatibility enhancement and sensing platelets that are less electrical negatively charged and with higher tendency to aggregate and form thrombus. The results are discussed in view of the effect of different deposition conditions of hydrogenated carbon films on their structural and morphological characteristics, surface roughness and electrical properties attributing to different hemocompatibility and sensing aspects.
format Text
id pubmed-2636585
institution National Center for Biotechnology Information
language English
publishDate 2008
publisher Dove Medical Press
record_format MEDLINE/PubMed
spelling pubmed-26365852009-04-01 Impact of surface electric properties of carbon-based thin films on platelets activation for nano-medical and nano-sensing applications Karagkiozaki, Varvara Logothetidis, Stergios Lousinian, Sylvie Giannoglou, George Int J Nanomedicine Original Research Electric surface properties of biomaterials, playing key role to various biointerfacial interactions, were related to hemocompatibility and biosensing phenomena. In this study, the examination of surface electric properties of amorphous hydrogenated carbon thin films (a-C:H) was carried out by means of electrostatic force microscope (EFM) and observation of differences in spatial charge distribution on the surface of the examined films during platelets adhesion was made. The thrombogenic potential of a-C:H thin films developed by magnetron sputtering with ~42% sp(3) content and hydrogen partial pressure during deposition was evaluated, by in situ observation with atomic force microscope (AFM) of platelets’ activation and their subsequent adhesion. Platelet-rich plasma drawn from healthy donors was used and semi-contact mode of AFM was applied. Platelets behavior and their correlation with the electric surface properties of the examined a-C:H films by EFM was made for hemocompatibility enhancement and sensing platelets that are less electrical negatively charged and with higher tendency to aggregate and form thrombus. The results are discussed in view of the effect of different deposition conditions of hydrogenated carbon films on their structural and morphological characteristics, surface roughness and electrical properties attributing to different hemocompatibility and sensing aspects. Dove Medical Press 2008-12 /pmc/articles/PMC2636585/ /pubmed/19337414 Text en © 2008 Dove Medical Press Limited. All rights reserved
spellingShingle Original Research
Karagkiozaki, Varvara
Logothetidis, Stergios
Lousinian, Sylvie
Giannoglou, George
Impact of surface electric properties of carbon-based thin films on platelets activation for nano-medical and nano-sensing applications
title Impact of surface electric properties of carbon-based thin films on platelets activation for nano-medical and nano-sensing applications
title_full Impact of surface electric properties of carbon-based thin films on platelets activation for nano-medical and nano-sensing applications
title_fullStr Impact of surface electric properties of carbon-based thin films on platelets activation for nano-medical and nano-sensing applications
title_full_unstemmed Impact of surface electric properties of carbon-based thin films on platelets activation for nano-medical and nano-sensing applications
title_short Impact of surface electric properties of carbon-based thin films on platelets activation for nano-medical and nano-sensing applications
title_sort impact of surface electric properties of carbon-based thin films on platelets activation for nano-medical and nano-sensing applications
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2636585/
https://www.ncbi.nlm.nih.gov/pubmed/19337414
work_keys_str_mv AT karagkiozakivarvara impactofsurfaceelectricpropertiesofcarbonbasedthinfilmsonplateletsactivationfornanomedicalandnanosensingapplications
AT logothetidisstergios impactofsurfaceelectricpropertiesofcarbonbasedthinfilmsonplateletsactivationfornanomedicalandnanosensingapplications
AT lousiniansylvie impactofsurfaceelectricpropertiesofcarbonbasedthinfilmsonplateletsactivationfornanomedicalandnanosensingapplications
AT giannoglougeorge impactofsurfaceelectricpropertiesofcarbonbasedthinfilmsonplateletsactivationfornanomedicalandnanosensingapplications