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Maternal Transfer and Protective Role of the Alternative Complement Components in Zebrafish Danio rerio
Embryos of most fish develop externally and are exposed to an aquatic environment full of potential pathogens, whereas they have little or only limited ability to mount an efficient and protective response. How fish embryos survive pathogenic attacks remains poorly defined. Here we demonstrate that...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2637980/ https://www.ncbi.nlm.nih.gov/pubmed/19223977 http://dx.doi.org/10.1371/journal.pone.0004498 |
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author | Wang, Zhiping Zhang, Shicui Tong, Zhou Li, Lei Wang, Guangfeng |
author_facet | Wang, Zhiping Zhang, Shicui Tong, Zhou Li, Lei Wang, Guangfeng |
author_sort | Wang, Zhiping |
collection | PubMed |
description | Embryos of most fish develop externally and are exposed to an aquatic environment full of potential pathogens, whereas they have little or only limited ability to mount an efficient and protective response. How fish embryos survive pathogenic attacks remains poorly defined. Here we demonstrate that the maternal immunization of female zebrafish with formalin-killed Aeromonas hydrophila causes a significant increase in C3 and Bf contents in the mother, a corresponding rise in the offspring, and induces a remarkable increase in the hemolytic activities in both the mother and offspring. In addition, the embryos derived from the immunized mother are significantly more tolerant to A. hydrophila challenge than those from the unimmunized fish, and blocking C3 and Bf activities by injection of the antibodies against C3 and Bf into the embryos render them more susceptible to A. hydrophila. These results clearly show that the protection of zebrafish embryos against A. hydrophila can be achieved by the maternally-transferred immunity of the complement system operating via the alternative pathway. This appears to be the first report providing in vivo evidences for the protective role of the alternative complement components in the early embryos of zebrafish, paving the way for insights into the in vivo function of other maternally-transferred factors in fish. |
format | Text |
id | pubmed-2637980 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-26379802009-02-18 Maternal Transfer and Protective Role of the Alternative Complement Components in Zebrafish Danio rerio Wang, Zhiping Zhang, Shicui Tong, Zhou Li, Lei Wang, Guangfeng PLoS One Research Article Embryos of most fish develop externally and are exposed to an aquatic environment full of potential pathogens, whereas they have little or only limited ability to mount an efficient and protective response. How fish embryos survive pathogenic attacks remains poorly defined. Here we demonstrate that the maternal immunization of female zebrafish with formalin-killed Aeromonas hydrophila causes a significant increase in C3 and Bf contents in the mother, a corresponding rise in the offspring, and induces a remarkable increase in the hemolytic activities in both the mother and offspring. In addition, the embryos derived from the immunized mother are significantly more tolerant to A. hydrophila challenge than those from the unimmunized fish, and blocking C3 and Bf activities by injection of the antibodies against C3 and Bf into the embryos render them more susceptible to A. hydrophila. These results clearly show that the protection of zebrafish embryos against A. hydrophila can be achieved by the maternally-transferred immunity of the complement system operating via the alternative pathway. This appears to be the first report providing in vivo evidences for the protective role of the alternative complement components in the early embryos of zebrafish, paving the way for insights into the in vivo function of other maternally-transferred factors in fish. Public Library of Science 2009-02-18 /pmc/articles/PMC2637980/ /pubmed/19223977 http://dx.doi.org/10.1371/journal.pone.0004498 Text en Wang et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Wang, Zhiping Zhang, Shicui Tong, Zhou Li, Lei Wang, Guangfeng Maternal Transfer and Protective Role of the Alternative Complement Components in Zebrafish Danio rerio |
title | Maternal Transfer and Protective Role of the Alternative Complement Components in Zebrafish Danio rerio
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title_full | Maternal Transfer and Protective Role of the Alternative Complement Components in Zebrafish Danio rerio
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title_fullStr | Maternal Transfer and Protective Role of the Alternative Complement Components in Zebrafish Danio rerio
|
title_full_unstemmed | Maternal Transfer and Protective Role of the Alternative Complement Components in Zebrafish Danio rerio
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title_short | Maternal Transfer and Protective Role of the Alternative Complement Components in Zebrafish Danio rerio
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title_sort | maternal transfer and protective role of the alternative complement components in zebrafish danio rerio |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2637980/ https://www.ncbi.nlm.nih.gov/pubmed/19223977 http://dx.doi.org/10.1371/journal.pone.0004498 |
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