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The effect of bacteriophages T4 and HAP1 on in vitro melanoma migration
BACKGROUND: The antibacterial activity of bacteriophages has been described rather well. However, knowledge about the direct interactions of bacteriophages with mammalian organisms and their other, i.e. non-antibacterial, activities in mammalian systems is quite scarce. It must be emphasised that ba...
Autores principales: | , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2009
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2639589/ https://www.ncbi.nlm.nih.gov/pubmed/19154575 http://dx.doi.org/10.1186/1471-2180-9-13 |
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author | Dąbrowska, Krystyna Skaradziński, Grzegorz Jończyk, Paulina Kurzępa, Aneta Wietrzyk, Joanna Owczarek, Barbara Żaczek, Maciej Świtała-Jeleń, Kinga Boratyński, Janusz Poźniak, Gryzelda Maciejewska, Magdalena Górski, Andrzej |
author_facet | Dąbrowska, Krystyna Skaradziński, Grzegorz Jończyk, Paulina Kurzępa, Aneta Wietrzyk, Joanna Owczarek, Barbara Żaczek, Maciej Świtała-Jeleń, Kinga Boratyński, Janusz Poźniak, Gryzelda Maciejewska, Magdalena Górski, Andrzej |
author_sort | Dąbrowska, Krystyna |
collection | PubMed |
description | BACKGROUND: The antibacterial activity of bacteriophages has been described rather well. However, knowledge about the direct interactions of bacteriophages with mammalian organisms and their other, i.e. non-antibacterial, activities in mammalian systems is quite scarce. It must be emphasised that bacteriophages are natural parasites of bacteria, which in turn are parasites or symbionts of mammals (including humans). Bacteriophages are constantly present in mammalian bodies and the environment in great amounts. On the other hand, the perspective of the possible use of bacteriophage preparations for antibacterial therapies in cancer patients generates a substantial need to investigate the effects of phages on cancer processes. RESULTS: In these studies the migration of human and mouse melanoma on fibronectin was inhibited by purified T4 and HAP1 bacteriophage preparations. The migration of human melanoma was also inhibited by the HAP1 phage preparation on matrigel. No response of either melanoma cell line to lipopolysaccharide was observed. Therefore the effect of the phage preparations cannot be attributed to lipopolysaccharide. No differences in the effects of T4 and HAP1 on melanoma migration were observed. CONCLUSION: We believe that these observations are of importance for any further attempts to use bacteriophage preparations in antibacterial treatment. The risk of antibiotic-resistant hospital infections strongly affects cancer patients and these results suggest the possibility of beneficial phage treatment. We also believe that they will contribute to the general understanding of bacteriophage biology, as bacteriophages, extremely ubiquitous entities, are in permanent contact with human organisms. |
format | Text |
id | pubmed-2639589 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-26395892009-02-11 The effect of bacteriophages T4 and HAP1 on in vitro melanoma migration Dąbrowska, Krystyna Skaradziński, Grzegorz Jończyk, Paulina Kurzępa, Aneta Wietrzyk, Joanna Owczarek, Barbara Żaczek, Maciej Świtała-Jeleń, Kinga Boratyński, Janusz Poźniak, Gryzelda Maciejewska, Magdalena Górski, Andrzej BMC Microbiol Research article BACKGROUND: The antibacterial activity of bacteriophages has been described rather well. However, knowledge about the direct interactions of bacteriophages with mammalian organisms and their other, i.e. non-antibacterial, activities in mammalian systems is quite scarce. It must be emphasised that bacteriophages are natural parasites of bacteria, which in turn are parasites or symbionts of mammals (including humans). Bacteriophages are constantly present in mammalian bodies and the environment in great amounts. On the other hand, the perspective of the possible use of bacteriophage preparations for antibacterial therapies in cancer patients generates a substantial need to investigate the effects of phages on cancer processes. RESULTS: In these studies the migration of human and mouse melanoma on fibronectin was inhibited by purified T4 and HAP1 bacteriophage preparations. The migration of human melanoma was also inhibited by the HAP1 phage preparation on matrigel. No response of either melanoma cell line to lipopolysaccharide was observed. Therefore the effect of the phage preparations cannot be attributed to lipopolysaccharide. No differences in the effects of T4 and HAP1 on melanoma migration were observed. CONCLUSION: We believe that these observations are of importance for any further attempts to use bacteriophage preparations in antibacterial treatment. The risk of antibiotic-resistant hospital infections strongly affects cancer patients and these results suggest the possibility of beneficial phage treatment. We also believe that they will contribute to the general understanding of bacteriophage biology, as bacteriophages, extremely ubiquitous entities, are in permanent contact with human organisms. BioMed Central 2009-01-20 /pmc/articles/PMC2639589/ /pubmed/19154575 http://dx.doi.org/10.1186/1471-2180-9-13 Text en Copyright ©2009 Dąbrowska et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research article Dąbrowska, Krystyna Skaradziński, Grzegorz Jończyk, Paulina Kurzępa, Aneta Wietrzyk, Joanna Owczarek, Barbara Żaczek, Maciej Świtała-Jeleń, Kinga Boratyński, Janusz Poźniak, Gryzelda Maciejewska, Magdalena Górski, Andrzej The effect of bacteriophages T4 and HAP1 on in vitro melanoma migration |
title | The effect of bacteriophages T4 and HAP1 on in vitro melanoma migration |
title_full | The effect of bacteriophages T4 and HAP1 on in vitro melanoma migration |
title_fullStr | The effect of bacteriophages T4 and HAP1 on in vitro melanoma migration |
title_full_unstemmed | The effect of bacteriophages T4 and HAP1 on in vitro melanoma migration |
title_short | The effect of bacteriophages T4 and HAP1 on in vitro melanoma migration |
title_sort | effect of bacteriophages t4 and hap1 on in vitro melanoma migration |
topic | Research article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2639589/ https://www.ncbi.nlm.nih.gov/pubmed/19154575 http://dx.doi.org/10.1186/1471-2180-9-13 |
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