Cargando…

The effect of bacteriophages T4 and HAP1 on in vitro melanoma migration

BACKGROUND: The antibacterial activity of bacteriophages has been described rather well. However, knowledge about the direct interactions of bacteriophages with mammalian organisms and their other, i.e. non-antibacterial, activities in mammalian systems is quite scarce. It must be emphasised that ba...

Descripción completa

Detalles Bibliográficos
Autores principales: Dąbrowska, Krystyna, Skaradziński, Grzegorz, Jończyk, Paulina, Kurzępa, Aneta, Wietrzyk, Joanna, Owczarek, Barbara, Żaczek, Maciej, Świtała-Jeleń, Kinga, Boratyński, Janusz, Poźniak, Gryzelda, Maciejewska, Magdalena, Górski, Andrzej
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2639589/
https://www.ncbi.nlm.nih.gov/pubmed/19154575
http://dx.doi.org/10.1186/1471-2180-9-13
_version_ 1782164479973261312
author Dąbrowska, Krystyna
Skaradziński, Grzegorz
Jończyk, Paulina
Kurzępa, Aneta
Wietrzyk, Joanna
Owczarek, Barbara
Żaczek, Maciej
Świtała-Jeleń, Kinga
Boratyński, Janusz
Poźniak, Gryzelda
Maciejewska, Magdalena
Górski, Andrzej
author_facet Dąbrowska, Krystyna
Skaradziński, Grzegorz
Jończyk, Paulina
Kurzępa, Aneta
Wietrzyk, Joanna
Owczarek, Barbara
Żaczek, Maciej
Świtała-Jeleń, Kinga
Boratyński, Janusz
Poźniak, Gryzelda
Maciejewska, Magdalena
Górski, Andrzej
author_sort Dąbrowska, Krystyna
collection PubMed
description BACKGROUND: The antibacterial activity of bacteriophages has been described rather well. However, knowledge about the direct interactions of bacteriophages with mammalian organisms and their other, i.e. non-antibacterial, activities in mammalian systems is quite scarce. It must be emphasised that bacteriophages are natural parasites of bacteria, which in turn are parasites or symbionts of mammals (including humans). Bacteriophages are constantly present in mammalian bodies and the environment in great amounts. On the other hand, the perspective of the possible use of bacteriophage preparations for antibacterial therapies in cancer patients generates a substantial need to investigate the effects of phages on cancer processes. RESULTS: In these studies the migration of human and mouse melanoma on fibronectin was inhibited by purified T4 and HAP1 bacteriophage preparations. The migration of human melanoma was also inhibited by the HAP1 phage preparation on matrigel. No response of either melanoma cell line to lipopolysaccharide was observed. Therefore the effect of the phage preparations cannot be attributed to lipopolysaccharide. No differences in the effects of T4 and HAP1 on melanoma migration were observed. CONCLUSION: We believe that these observations are of importance for any further attempts to use bacteriophage preparations in antibacterial treatment. The risk of antibiotic-resistant hospital infections strongly affects cancer patients and these results suggest the possibility of beneficial phage treatment. We also believe that they will contribute to the general understanding of bacteriophage biology, as bacteriophages, extremely ubiquitous entities, are in permanent contact with human organisms.
format Text
id pubmed-2639589
institution National Center for Biotechnology Information
language English
publishDate 2009
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-26395892009-02-11 The effect of bacteriophages T4 and HAP1 on in vitro melanoma migration Dąbrowska, Krystyna Skaradziński, Grzegorz Jończyk, Paulina Kurzępa, Aneta Wietrzyk, Joanna Owczarek, Barbara Żaczek, Maciej Świtała-Jeleń, Kinga Boratyński, Janusz Poźniak, Gryzelda Maciejewska, Magdalena Górski, Andrzej BMC Microbiol Research article BACKGROUND: The antibacterial activity of bacteriophages has been described rather well. However, knowledge about the direct interactions of bacteriophages with mammalian organisms and their other, i.e. non-antibacterial, activities in mammalian systems is quite scarce. It must be emphasised that bacteriophages are natural parasites of bacteria, which in turn are parasites or symbionts of mammals (including humans). Bacteriophages are constantly present in mammalian bodies and the environment in great amounts. On the other hand, the perspective of the possible use of bacteriophage preparations for antibacterial therapies in cancer patients generates a substantial need to investigate the effects of phages on cancer processes. RESULTS: In these studies the migration of human and mouse melanoma on fibronectin was inhibited by purified T4 and HAP1 bacteriophage preparations. The migration of human melanoma was also inhibited by the HAP1 phage preparation on matrigel. No response of either melanoma cell line to lipopolysaccharide was observed. Therefore the effect of the phage preparations cannot be attributed to lipopolysaccharide. No differences in the effects of T4 and HAP1 on melanoma migration were observed. CONCLUSION: We believe that these observations are of importance for any further attempts to use bacteriophage preparations in antibacterial treatment. The risk of antibiotic-resistant hospital infections strongly affects cancer patients and these results suggest the possibility of beneficial phage treatment. We also believe that they will contribute to the general understanding of bacteriophage biology, as bacteriophages, extremely ubiquitous entities, are in permanent contact with human organisms. BioMed Central 2009-01-20 /pmc/articles/PMC2639589/ /pubmed/19154575 http://dx.doi.org/10.1186/1471-2180-9-13 Text en Copyright ©2009 Dąbrowska et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research article
Dąbrowska, Krystyna
Skaradziński, Grzegorz
Jończyk, Paulina
Kurzępa, Aneta
Wietrzyk, Joanna
Owczarek, Barbara
Żaczek, Maciej
Świtała-Jeleń, Kinga
Boratyński, Janusz
Poźniak, Gryzelda
Maciejewska, Magdalena
Górski, Andrzej
The effect of bacteriophages T4 and HAP1 on in vitro melanoma migration
title The effect of bacteriophages T4 and HAP1 on in vitro melanoma migration
title_full The effect of bacteriophages T4 and HAP1 on in vitro melanoma migration
title_fullStr The effect of bacteriophages T4 and HAP1 on in vitro melanoma migration
title_full_unstemmed The effect of bacteriophages T4 and HAP1 on in vitro melanoma migration
title_short The effect of bacteriophages T4 and HAP1 on in vitro melanoma migration
title_sort effect of bacteriophages t4 and hap1 on in vitro melanoma migration
topic Research article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2639589/
https://www.ncbi.nlm.nih.gov/pubmed/19154575
http://dx.doi.org/10.1186/1471-2180-9-13
work_keys_str_mv AT dabrowskakrystyna theeffectofbacteriophagest4andhap1oninvitromelanomamigration
AT skaradzinskigrzegorz theeffectofbacteriophagest4andhap1oninvitromelanomamigration
AT jonczykpaulina theeffectofbacteriophagest4andhap1oninvitromelanomamigration
AT kurzepaaneta theeffectofbacteriophagest4andhap1oninvitromelanomamigration
AT wietrzykjoanna theeffectofbacteriophagest4andhap1oninvitromelanomamigration
AT owczarekbarbara theeffectofbacteriophagest4andhap1oninvitromelanomamigration
AT zaczekmaciej theeffectofbacteriophagest4andhap1oninvitromelanomamigration
AT switałajelenkinga theeffectofbacteriophagest4andhap1oninvitromelanomamigration
AT boratynskijanusz theeffectofbacteriophagest4andhap1oninvitromelanomamigration
AT pozniakgryzelda theeffectofbacteriophagest4andhap1oninvitromelanomamigration
AT maciejewskamagdalena theeffectofbacteriophagest4andhap1oninvitromelanomamigration
AT gorskiandrzej theeffectofbacteriophagest4andhap1oninvitromelanomamigration
AT dabrowskakrystyna effectofbacteriophagest4andhap1oninvitromelanomamigration
AT skaradzinskigrzegorz effectofbacteriophagest4andhap1oninvitromelanomamigration
AT jonczykpaulina effectofbacteriophagest4andhap1oninvitromelanomamigration
AT kurzepaaneta effectofbacteriophagest4andhap1oninvitromelanomamigration
AT wietrzykjoanna effectofbacteriophagest4andhap1oninvitromelanomamigration
AT owczarekbarbara effectofbacteriophagest4andhap1oninvitromelanomamigration
AT zaczekmaciej effectofbacteriophagest4andhap1oninvitromelanomamigration
AT switałajelenkinga effectofbacteriophagest4andhap1oninvitromelanomamigration
AT boratynskijanusz effectofbacteriophagest4andhap1oninvitromelanomamigration
AT pozniakgryzelda effectofbacteriophagest4andhap1oninvitromelanomamigration
AT maciejewskamagdalena effectofbacteriophagest4andhap1oninvitromelanomamigration
AT gorskiandrzej effectofbacteriophagest4andhap1oninvitromelanomamigration