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Molecular recognition of acute myeloid leukemia using aptamers

Cell surface proteins can play important roles in cancer pathogenesis. Comprehensive understanding of the surface protein expression patterns of tumor cells and, consequently, the pathogenesis of tumor cells, depends on molecular probes against these proteins. To be effectively used for tumor diagno...

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Autores principales: Sefah, Kwame, Tang, Zhiwen, Shangguan, Dihua, Chen, Hui, Lopez-Colon, Dalia, Li, Ying, Parekh, Parag, Martin, Jennifer, Meng, Ling, Tan, Weihong
Formato: Texto
Lenguaje:English
Publicado: 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2640431/
https://www.ncbi.nlm.nih.gov/pubmed/19151784
http://dx.doi.org/10.1038/leu.2008.335
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author Sefah, Kwame
Tang, Zhiwen
Shangguan, Dihua
Chen, Hui
Lopez-Colon, Dalia
Li, Ying
Parekh, Parag
Martin, Jennifer
Meng, Ling
Tan, Weihong
author_facet Sefah, Kwame
Tang, Zhiwen
Shangguan, Dihua
Chen, Hui
Lopez-Colon, Dalia
Li, Ying
Parekh, Parag
Martin, Jennifer
Meng, Ling
Tan, Weihong
author_sort Sefah, Kwame
collection PubMed
description Cell surface proteins can play important roles in cancer pathogenesis. Comprehensive understanding of the surface protein expression patterns of tumor cells and, consequently, the pathogenesis of tumor cells, depends on molecular probes against these proteins. To be effectively used for tumor diagnosis, classification and therapy, such probes would be capable of specific binding to targeted tumor cells. Molecular aptamers, designer DNA/RNA probes, can address this challenge by recognizing proteins, peptides and other small molecules with high affinity and specificity. Through a process known as cell-SELEX, we used live acute myeloid leukemia (AML) cells to select a group of DNA aptamers that can recognize acute myeloid leukemia cells with dissociation constants (Kds) in the nanomolar range. Interestingly, one aptamer (KH1C12), compared with two control cell lines (K562 and NB4), showed significant selectivity to the target AML cell line (HL60) and could recognize the target cells within a complex mixture of normal bone marrow aspirates. The other two aptamers KK1B10 and KK1D04 recognize targets associated with monocytic differentiation. Our studies demonstrate that the selected aptamers can be used as a molecular tool for further understanding surface protein expression patterns on tumor cells and thus providing a foundation for effective molecular analysis of leukemia and its subcategories.
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spelling pubmed-26404312009-08-01 Molecular recognition of acute myeloid leukemia using aptamers Sefah, Kwame Tang, Zhiwen Shangguan, Dihua Chen, Hui Lopez-Colon, Dalia Li, Ying Parekh, Parag Martin, Jennifer Meng, Ling Tan, Weihong Leukemia Article Cell surface proteins can play important roles in cancer pathogenesis. Comprehensive understanding of the surface protein expression patterns of tumor cells and, consequently, the pathogenesis of tumor cells, depends on molecular probes against these proteins. To be effectively used for tumor diagnosis, classification and therapy, such probes would be capable of specific binding to targeted tumor cells. Molecular aptamers, designer DNA/RNA probes, can address this challenge by recognizing proteins, peptides and other small molecules with high affinity and specificity. Through a process known as cell-SELEX, we used live acute myeloid leukemia (AML) cells to select a group of DNA aptamers that can recognize acute myeloid leukemia cells with dissociation constants (Kds) in the nanomolar range. Interestingly, one aptamer (KH1C12), compared with two control cell lines (K562 and NB4), showed significant selectivity to the target AML cell line (HL60) and could recognize the target cells within a complex mixture of normal bone marrow aspirates. The other two aptamers KK1B10 and KK1D04 recognize targets associated with monocytic differentiation. Our studies demonstrate that the selected aptamers can be used as a molecular tool for further understanding surface protein expression patterns on tumor cells and thus providing a foundation for effective molecular analysis of leukemia and its subcategories. 2009-01-08 2009-02 /pmc/articles/PMC2640431/ /pubmed/19151784 http://dx.doi.org/10.1038/leu.2008.335 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Sefah, Kwame
Tang, Zhiwen
Shangguan, Dihua
Chen, Hui
Lopez-Colon, Dalia
Li, Ying
Parekh, Parag
Martin, Jennifer
Meng, Ling
Tan, Weihong
Molecular recognition of acute myeloid leukemia using aptamers
title Molecular recognition of acute myeloid leukemia using aptamers
title_full Molecular recognition of acute myeloid leukemia using aptamers
title_fullStr Molecular recognition of acute myeloid leukemia using aptamers
title_full_unstemmed Molecular recognition of acute myeloid leukemia using aptamers
title_short Molecular recognition of acute myeloid leukemia using aptamers
title_sort molecular recognition of acute myeloid leukemia using aptamers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2640431/
https://www.ncbi.nlm.nih.gov/pubmed/19151784
http://dx.doi.org/10.1038/leu.2008.335
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