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Cell-Autonomous Requirement for Rx Function in the Mammalian Retina and Posterior Pituitary
Rx is a paired-like homeobox gene that is required for vertebrate eye formation. Mice lacking Rx function do not develop eyes or the posterior pituitary. To determine whether Rx is required cell autonomously in these tissues, we generated embryonic chimeras consisting of wild type and Rx−/− cells. W...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2641000/ https://www.ncbi.nlm.nih.gov/pubmed/19229337 http://dx.doi.org/10.1371/journal.pone.0004513 |
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author | Medina-Martinez, Olga Amaya-Manzanares, Felipe Liu, Chaomei Mendoza, Marisela Shah, Rina Zhang, Li Behringer, Richard R. Mahon, Kathleen A. Jamrich, Milan |
author_facet | Medina-Martinez, Olga Amaya-Manzanares, Felipe Liu, Chaomei Mendoza, Marisela Shah, Rina Zhang, Li Behringer, Richard R. Mahon, Kathleen A. Jamrich, Milan |
author_sort | Medina-Martinez, Olga |
collection | PubMed |
description | Rx is a paired-like homeobox gene that is required for vertebrate eye formation. Mice lacking Rx function do not develop eyes or the posterior pituitary. To determine whether Rx is required cell autonomously in these tissues, we generated embryonic chimeras consisting of wild type and Rx−/− cells. We found that in the eye, Rx-deficient cells cannot participate in the formation of the neuroretina, retina pigment epithelium and the distal part of the optic stalk. In addition, in the ventral forebrain, Rx function is required cell autonomously for the formation of the posterior pituitary. Interestingly, Rx−/− and wild type cells segregate before the morphogenesis of these two tissues begins. Our observations suggest that Rx function is not only required for the morphogenesis of the retina and posterior pituitary, but also prior to morphogenesis, for the sorting out of cells to form distinct fields of retinal/pituitary cells. |
format | Text |
id | pubmed-2641000 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-26410002009-02-20 Cell-Autonomous Requirement for Rx Function in the Mammalian Retina and Posterior Pituitary Medina-Martinez, Olga Amaya-Manzanares, Felipe Liu, Chaomei Mendoza, Marisela Shah, Rina Zhang, Li Behringer, Richard R. Mahon, Kathleen A. Jamrich, Milan PLoS One Research Article Rx is a paired-like homeobox gene that is required for vertebrate eye formation. Mice lacking Rx function do not develop eyes or the posterior pituitary. To determine whether Rx is required cell autonomously in these tissues, we generated embryonic chimeras consisting of wild type and Rx−/− cells. We found that in the eye, Rx-deficient cells cannot participate in the formation of the neuroretina, retina pigment epithelium and the distal part of the optic stalk. In addition, in the ventral forebrain, Rx function is required cell autonomously for the formation of the posterior pituitary. Interestingly, Rx−/− and wild type cells segregate before the morphogenesis of these two tissues begins. Our observations suggest that Rx function is not only required for the morphogenesis of the retina and posterior pituitary, but also prior to morphogenesis, for the sorting out of cells to form distinct fields of retinal/pituitary cells. Public Library of Science 2009-02-20 /pmc/articles/PMC2641000/ /pubmed/19229337 http://dx.doi.org/10.1371/journal.pone.0004513 Text en Medina-Martinez et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Medina-Martinez, Olga Amaya-Manzanares, Felipe Liu, Chaomei Mendoza, Marisela Shah, Rina Zhang, Li Behringer, Richard R. Mahon, Kathleen A. Jamrich, Milan Cell-Autonomous Requirement for Rx Function in the Mammalian Retina and Posterior Pituitary |
title | Cell-Autonomous Requirement for Rx Function in the Mammalian Retina and Posterior Pituitary |
title_full | Cell-Autonomous Requirement for Rx Function in the Mammalian Retina and Posterior Pituitary |
title_fullStr | Cell-Autonomous Requirement for Rx Function in the Mammalian Retina and Posterior Pituitary |
title_full_unstemmed | Cell-Autonomous Requirement for Rx Function in the Mammalian Retina and Posterior Pituitary |
title_short | Cell-Autonomous Requirement for Rx Function in the Mammalian Retina and Posterior Pituitary |
title_sort | cell-autonomous requirement for rx function in the mammalian retina and posterior pituitary |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2641000/ https://www.ncbi.nlm.nih.gov/pubmed/19229337 http://dx.doi.org/10.1371/journal.pone.0004513 |
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