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In vivo killing of Staphylococcus aureus using a light-activated antimicrobial agent
BACKGROUND: The widespread problem of antibiotic resistance in pathogens such as Staphylococcus aureus has prompted the search for new antimicrobial approaches. In this study we report for the first time the use of a light-activated antimicrobial agent, methylene blue, to kill an epidemic methicilli...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2642833/ https://www.ncbi.nlm.nih.gov/pubmed/19193212 http://dx.doi.org/10.1186/1471-2180-9-27 |
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author | Zolfaghari, Parjam S Packer, Samantha Singer, Mervyn Nair, Sean P Bennett, Jon Street, Cale Wilson, Michael |
author_facet | Zolfaghari, Parjam S Packer, Samantha Singer, Mervyn Nair, Sean P Bennett, Jon Street, Cale Wilson, Michael |
author_sort | Zolfaghari, Parjam S |
collection | PubMed |
description | BACKGROUND: The widespread problem of antibiotic resistance in pathogens such as Staphylococcus aureus has prompted the search for new antimicrobial approaches. In this study we report for the first time the use of a light-activated antimicrobial agent, methylene blue, to kill an epidemic methicillin-resistant Staphylococcus aureus (EMRSA-16) strain in two mouse wound models. RESULTS: Following irradiation of wounds with 360 J/cm(2 )of laser light (670 nm) in the presence of 100 μg/ml of methylene blue, a 25-fold reduction in the number of viable EMRSA was seen. This was independent of the increase in temperature of the wounds associated with the treatment. Histological examination of the wounds revealed no difference between the photodynamic therapy (PDT)-treated wounds and the untreated wounds, all of which showed the same degree of inflammatory infiltration at 24 hours. CONCLUSION: The results of this study demonstrate that PDT is effective at reducing the total number of viable EMRSA in a wound. This approach has promise as a means of treating wound infections caused by antibiotic-resistant microbes as well as for the elimination of such organisms from carriage sites. |
format | Text |
id | pubmed-2642833 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-26428332009-02-14 In vivo killing of Staphylococcus aureus using a light-activated antimicrobial agent Zolfaghari, Parjam S Packer, Samantha Singer, Mervyn Nair, Sean P Bennett, Jon Street, Cale Wilson, Michael BMC Microbiol Research article BACKGROUND: The widespread problem of antibiotic resistance in pathogens such as Staphylococcus aureus has prompted the search for new antimicrobial approaches. In this study we report for the first time the use of a light-activated antimicrobial agent, methylene blue, to kill an epidemic methicillin-resistant Staphylococcus aureus (EMRSA-16) strain in two mouse wound models. RESULTS: Following irradiation of wounds with 360 J/cm(2 )of laser light (670 nm) in the presence of 100 μg/ml of methylene blue, a 25-fold reduction in the number of viable EMRSA was seen. This was independent of the increase in temperature of the wounds associated with the treatment. Histological examination of the wounds revealed no difference between the photodynamic therapy (PDT)-treated wounds and the untreated wounds, all of which showed the same degree of inflammatory infiltration at 24 hours. CONCLUSION: The results of this study demonstrate that PDT is effective at reducing the total number of viable EMRSA in a wound. This approach has promise as a means of treating wound infections caused by antibiotic-resistant microbes as well as for the elimination of such organisms from carriage sites. BioMed Central 2009-02-04 /pmc/articles/PMC2642833/ /pubmed/19193212 http://dx.doi.org/10.1186/1471-2180-9-27 Text en Copyright ©2009 Zolfaghari et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research article Zolfaghari, Parjam S Packer, Samantha Singer, Mervyn Nair, Sean P Bennett, Jon Street, Cale Wilson, Michael In vivo killing of Staphylococcus aureus using a light-activated antimicrobial agent |
title | In vivo killing of Staphylococcus aureus using a light-activated antimicrobial agent |
title_full | In vivo killing of Staphylococcus aureus using a light-activated antimicrobial agent |
title_fullStr | In vivo killing of Staphylococcus aureus using a light-activated antimicrobial agent |
title_full_unstemmed | In vivo killing of Staphylococcus aureus using a light-activated antimicrobial agent |
title_short | In vivo killing of Staphylococcus aureus using a light-activated antimicrobial agent |
title_sort | in vivo killing of staphylococcus aureus using a light-activated antimicrobial agent |
topic | Research article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2642833/ https://www.ncbi.nlm.nih.gov/pubmed/19193212 http://dx.doi.org/10.1186/1471-2180-9-27 |
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