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In vivo killing of Staphylococcus aureus using a light-activated antimicrobial agent

BACKGROUND: The widespread problem of antibiotic resistance in pathogens such as Staphylococcus aureus has prompted the search for new antimicrobial approaches. In this study we report for the first time the use of a light-activated antimicrobial agent, methylene blue, to kill an epidemic methicilli...

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Autores principales: Zolfaghari, Parjam S, Packer, Samantha, Singer, Mervyn, Nair, Sean P, Bennett, Jon, Street, Cale, Wilson, Michael
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2642833/
https://www.ncbi.nlm.nih.gov/pubmed/19193212
http://dx.doi.org/10.1186/1471-2180-9-27
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author Zolfaghari, Parjam S
Packer, Samantha
Singer, Mervyn
Nair, Sean P
Bennett, Jon
Street, Cale
Wilson, Michael
author_facet Zolfaghari, Parjam S
Packer, Samantha
Singer, Mervyn
Nair, Sean P
Bennett, Jon
Street, Cale
Wilson, Michael
author_sort Zolfaghari, Parjam S
collection PubMed
description BACKGROUND: The widespread problem of antibiotic resistance in pathogens such as Staphylococcus aureus has prompted the search for new antimicrobial approaches. In this study we report for the first time the use of a light-activated antimicrobial agent, methylene blue, to kill an epidemic methicillin-resistant Staphylococcus aureus (EMRSA-16) strain in two mouse wound models. RESULTS: Following irradiation of wounds with 360 J/cm(2 )of laser light (670 nm) in the presence of 100 μg/ml of methylene blue, a 25-fold reduction in the number of viable EMRSA was seen. This was independent of the increase in temperature of the wounds associated with the treatment. Histological examination of the wounds revealed no difference between the photodynamic therapy (PDT)-treated wounds and the untreated wounds, all of which showed the same degree of inflammatory infiltration at 24 hours. CONCLUSION: The results of this study demonstrate that PDT is effective at reducing the total number of viable EMRSA in a wound. This approach has promise as a means of treating wound infections caused by antibiotic-resistant microbes as well as for the elimination of such organisms from carriage sites.
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spelling pubmed-26428332009-02-14 In vivo killing of Staphylococcus aureus using a light-activated antimicrobial agent Zolfaghari, Parjam S Packer, Samantha Singer, Mervyn Nair, Sean P Bennett, Jon Street, Cale Wilson, Michael BMC Microbiol Research article BACKGROUND: The widespread problem of antibiotic resistance in pathogens such as Staphylococcus aureus has prompted the search for new antimicrobial approaches. In this study we report for the first time the use of a light-activated antimicrobial agent, methylene blue, to kill an epidemic methicillin-resistant Staphylococcus aureus (EMRSA-16) strain in two mouse wound models. RESULTS: Following irradiation of wounds with 360 J/cm(2 )of laser light (670 nm) in the presence of 100 μg/ml of methylene blue, a 25-fold reduction in the number of viable EMRSA was seen. This was independent of the increase in temperature of the wounds associated with the treatment. Histological examination of the wounds revealed no difference between the photodynamic therapy (PDT)-treated wounds and the untreated wounds, all of which showed the same degree of inflammatory infiltration at 24 hours. CONCLUSION: The results of this study demonstrate that PDT is effective at reducing the total number of viable EMRSA in a wound. This approach has promise as a means of treating wound infections caused by antibiotic-resistant microbes as well as for the elimination of such organisms from carriage sites. BioMed Central 2009-02-04 /pmc/articles/PMC2642833/ /pubmed/19193212 http://dx.doi.org/10.1186/1471-2180-9-27 Text en Copyright ©2009 Zolfaghari et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research article
Zolfaghari, Parjam S
Packer, Samantha
Singer, Mervyn
Nair, Sean P
Bennett, Jon
Street, Cale
Wilson, Michael
In vivo killing of Staphylococcus aureus using a light-activated antimicrobial agent
title In vivo killing of Staphylococcus aureus using a light-activated antimicrobial agent
title_full In vivo killing of Staphylococcus aureus using a light-activated antimicrobial agent
title_fullStr In vivo killing of Staphylococcus aureus using a light-activated antimicrobial agent
title_full_unstemmed In vivo killing of Staphylococcus aureus using a light-activated antimicrobial agent
title_short In vivo killing of Staphylococcus aureus using a light-activated antimicrobial agent
title_sort in vivo killing of staphylococcus aureus using a light-activated antimicrobial agent
topic Research article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2642833/
https://www.ncbi.nlm.nih.gov/pubmed/19193212
http://dx.doi.org/10.1186/1471-2180-9-27
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